Experiment: P. simiae OR Antrim MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_21075 and PS417_21080 are separated by 40 nucleotides PS417_21080 and PS417_21085 overlap by 1 nucleotides PS417_21085 and PS417_21090 are separated by 272 nucleotides PS417_21090 and PS417_21095 are separated by 5 nucleotides
PS417_21075: PS417_21075 - methylcitrate synthase, at 4,587,428 to 4,588,555
_21075
PS417_21080: PS417_21080 - 2-methylisocitrate lyase, at 4,588,596 to 4,589,489
_21080
PS417_21085: PS417_21085 - GntR family transcriptional regulator, at 4,589,489 to 4,590,199
_21085
PS417_21090: PS417_21090 - hypothetical protein, at 4,590,472 to 4,591,008
_21090
PS417_21095: PS417_21095 - membrane protein, at 4,591,014 to 4,592,546
_21095
Position (kb)
4589
4590
4591 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4588.579 kb on - strand at 4588.598 kb on - strand at 4589.729 kb on - strand, within PS417_21085 at 4589.729 kb on - strand, within PS417_21085 at 4589.729 kb on - strand, within PS417_21085 at 4589.788 kb on - strand, within PS417_21085 at 4589.926 kb on - strand, within PS417_21085 at 4590.052 kb on - strand, within PS417_21085 at 4590.358 kb on + strand at 4590.422 kb on + strand at 4590.423 kb on - strand at 4590.471 kb on + strand at 4590.471 kb on + strand at 4590.494 kb on + strand at 4590.551 kb on - strand, within PS417_21090 at 4590.566 kb on - strand, within PS417_21090 at 4590.800 kb on - strand, within PS417_21090 at 4591.071 kb on - strand at 4591.071 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae OR Antrim MOI 1 remove 4,588,579 - +1.3 4,588,598 - +0.9 4,589,729 - PS417_21085 0.34 +0.3 4,589,729 - PS417_21085 0.34 +3.0 4,589,729 - PS417_21085 0.34 +0.4 4,589,788 - PS417_21085 0.42 -0.1 4,589,926 - PS417_21085 0.61 +0.5 4,590,052 - PS417_21085 0.79 +1.3 4,590,358 + -2.6 4,590,422 + -2.3 4,590,423 - -1.7 4,590,471 + +0.6 4,590,471 + -1.9 4,590,494 + -0.2 4,590,551 - PS417_21090 0.15 -1.5 4,590,566 - PS417_21090 0.18 +0.4 4,590,800 - PS417_21090 0.61 +2.1 4,591,071 - -0.1 4,591,071 - -0.3
Or see this region's nucleotide sequence