Experiment: P. simiae OR Antrim MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12605 and PS417_12610 overlap by 4 nucleotides PS417_12610 and PS417_12615 are separated by 170 nucleotides PS417_12615 and PS417_12620 are separated by 138 nucleotides
PS417_12605: PS417_12605 - RNA polymerase subunit sigma-70, at 2,744,470 to 2,745,138
_12605
PS417_12610: PS417_12610 - RNA polymerase sigma factor, at 2,745,135 to 2,745,650
_12610
PS417_12615: PS417_12615 - hypothetical protein, at 2,745,821 to 2,746,342
_12615
PS417_12620: PS417_12620 - SAM-dependent methyltransferase, at 2,746,481 to 2,747,251
_12620
Position (kb)
2745
2746
2747 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 2744.931 kb on + strand at 2744.931 kb on + strand, within PS417_12605 at 2745.104 kb on + strand at 2745.104 kb on + strand at 2745.105 kb on - strand at 2745.105 kb on - strand at 2745.247 kb on + strand, within PS417_12610 at 2745.247 kb on + strand, within PS417_12610 at 2745.397 kb on - strand, within PS417_12610 at 2745.583 kb on + strand at 2745.584 kb on - strand, within PS417_12610 at 2745.652 kb on + strand at 2745.652 kb on + strand at 2745.652 kb on + strand at 2745.653 kb on - strand at 2745.653 kb on - strand at 2745.653 kb on - strand at 2745.684 kb on - strand at 2745.684 kb on - strand at 2745.684 kb on - strand at 2745.777 kb on + strand at 2745.777 kb on + strand at 2745.784 kb on + strand at 2745.784 kb on + strand at 2745.784 kb on + strand at 2745.784 kb on + strand at 2745.784 kb on + strand at 2745.784 kb on + strand at 2745.785 kb on - strand at 2745.785 kb on - strand at 2745.785 kb on - strand at 2745.785 kb on - strand at 2745.785 kb on - strand at 2745.785 kb on - strand at 2745.785 kb on - strand at 2745.808 kb on + strand at 2745.808 kb on + strand at 2745.943 kb on - strand, within PS417_12615 at 2746.095 kb on + strand, within PS417_12615 at 2746.095 kb on + strand, within PS417_12615 at 2746.198 kb on - strand, within PS417_12615 at 2746.198 kb on - strand, within PS417_12615 at 2746.312 kb on + strand at 2746.334 kb on - strand at 2746.347 kb on - strand at 2746.362 kb on + strand at 2746.491 kb on + strand at 2746.491 kb on + strand at 2746.492 kb on - strand at 2746.492 kb on - strand at 2746.492 kb on - strand at 2746.946 kb on - strand, within PS417_12620 at 2747.035 kb on - strand, within PS417_12620 at 2747.035 kb on - strand, within PS417_12620 at 2747.228 kb on + strand at 2747.228 kb on + strand at 2747.228 kb on + strand at 2747.229 kb on - strand at 2747.229 kb on - strand at 2747.229 kb on - strand at 2747.229 kb on - strand at 2747.278 kb on - strand at 2747.280 kb on - strand at 2747.323 kb on + strand at 2747.335 kb on + strand at 2747.336 kb on - strand at 2747.336 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae OR Antrim MOI 1 remove 2,744,931 + +3.4 2,744,931 + PS417_12605 0.69 -0.2 2,745,104 + -2.1 2,745,104 + +0.5 2,745,105 - -0.5 2,745,105 - -0.0 2,745,247 + PS417_12610 0.22 +0.1 2,745,247 + PS417_12610 0.22 +0.3 2,745,397 - PS417_12610 0.51 -0.1 2,745,583 + +0.4 2,745,584 - PS417_12610 0.87 +0.2 2,745,652 + +1.2 2,745,652 + -1.8 2,745,652 + -0.2 2,745,653 - +0.1 2,745,653 - -0.9 2,745,653 - -0.6 2,745,684 - +1.4 2,745,684 - -0.5 2,745,684 - -0.1 2,745,777 + -0.6 2,745,777 + +0.2 2,745,784 + -0.9 2,745,784 + -0.1 2,745,784 + -0.4 2,745,784 + -0.7 2,745,784 + -0.1 2,745,784 + +2.3 2,745,785 - +0.2 2,745,785 - -0.1 2,745,785 - +0.7 2,745,785 - +0.4 2,745,785 - +0.0 2,745,785 - +0.3 2,745,785 - -0.5 2,745,808 + +0.1 2,745,808 + -0.1 2,745,943 - PS417_12615 0.23 -0.3 2,746,095 + PS417_12615 0.52 -0.6 2,746,095 + PS417_12615 0.52 -0.4 2,746,198 - PS417_12615 0.72 -0.6 2,746,198 - PS417_12615 0.72 -0.6 2,746,312 + +1.7 2,746,334 - +0.3 2,746,347 - -0.6 2,746,362 + -0.4 2,746,491 + -0.7 2,746,491 + -0.0 2,746,492 - -0.1 2,746,492 - +1.1 2,746,492 - -1.3 2,746,946 - PS417_12620 0.60 +0.7 2,747,035 - PS417_12620 0.72 -0.1 2,747,035 - PS417_12620 0.72 -0.8 2,747,228 + +5.0 2,747,228 + -0.9 2,747,228 + +0.2 2,747,229 - -0.1 2,747,229 - -0.9 2,747,229 - +0.2 2,747,229 - -0.2 2,747,278 - +1.4 2,747,280 - -0.4 2,747,323 + -1.5 2,747,335 + +2.4 2,747,336 - +1.6 2,747,336 - -0.7
Or see this region's nucleotide sequence