Strain Fitness in Pseudomonas putida KT2440 around PP_0149

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0147 and PP_0148 are separated by 264 nucleotidesPP_0148 and PP_0149 are separated by 38 nucleotidesPP_0149 and PP_0150 overlap by 4 nucleotidesPP_0150 and PP_0151 are separated by 36 nucleotidesPP_0151 and PP_0152 are separated by 89 nucleotides PP_0147: PP_0147 - Citrate transporter, at 156,418 to 157,725 _0147 PP_0148: PP_0148 - conserved protein of unknown function, at 157,990 to 158,394 _0148 PP_0149: PP_0149 - conserved exported protein of unknown function, at 158,433 to 160,388 _0149 PP_0150: PP_0150 - conserved exported protein of unknown function, at 160,385 to 160,705 _0150 PP_0151: PP_0151 - conserved exported protein of unknown function, at 160,742 to 161,035 _0151 PP_0152: PP_0152 - conserved exported protein of unknown function, at 161,125 to 161,505 _0152 Position (kb) 158 159 160 161Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 157.520 kb on + strand, within PP_0147at 157.521 kb on - strand, within PP_0147at 157.577 kb on - strand, within PP_0147at 157.682 kb on + strandat 157.682 kb on + strandat 157.682 kb on + strandat 157.682 kb on + strandat 157.683 kb on - strandat 157.731 kb on + strandat 157.731 kb on + strandat 157.731 kb on + strandat 157.731 kb on + strandat 157.732 kb on - strandat 157.817 kb on + strandat 157.839 kb on + strandat 157.839 kb on + strandat 157.839 kb on + strandat 157.839 kb on + strandat 157.839 kb on + strandat 157.839 kb on + strandat 157.839 kb on + strandat 157.840 kb on - strandat 157.840 kb on - strandat 157.840 kb on - strandat 157.840 kb on - strandat 157.840 kb on - strandat 157.922 kb on - strandat 157.936 kb on + strandat 157.936 kb on + strandat 157.936 kb on + strandat 157.987 kb on + strandat 157.987 kb on + strandat 157.988 kb on - strandat 158.004 kb on - strandat 158.004 kb on - strandat 158.175 kb on + strand, within PP_0148at 158.176 kb on + strand, within PP_0148at 158.176 kb on + strand, within PP_0148at 158.176 kb on + strand, within PP_0148at 158.176 kb on + strand, within PP_0148at 158.176 kb on + strand, within PP_0148at 158.176 kb on + strand, within PP_0148at 158.176 kb on + strand, within PP_0148at 158.177 kb on - strand, within PP_0148at 158.177 kb on - strand, within PP_0148at 158.177 kb on - strand, within PP_0148at 158.177 kb on - strand, within PP_0148at 158.203 kb on + strand, within PP_0148at 158.251 kb on - strand, within PP_0148at 158.304 kb on - strand, within PP_0148at 158.319 kb on + strand, within PP_0148at 158.319 kb on + strandat 158.319 kb on + strand, within PP_0148at 158.319 kb on + strand, within PP_0148at 158.319 kb on + strand, within PP_0148at 158.320 kb on - strand, within PP_0148at 158.320 kb on - strand, within PP_0148at 158.320 kb on - strand, within PP_0148at 158.320 kb on - strand, within PP_0148at 158.320 kb on - strand, within PP_0148at 158.393 kb on - strandat 158.429 kb on + strandat 158.429 kb on + strandat 158.429 kb on + strandat 158.429 kb on + strandat 158.429 kb on + strandat 158.430 kb on - strandat 158.430 kb on - strandat 158.430 kb on - strandat 158.430 kb on - strandat 158.430 kb on - strandat 158.430 kb on - strandat 158.430 kb on - strandat 158.430 kb on - strandat 158.430 kb on - strandat 158.430 kb on - strandat 158.430 kb on - strandat 158.770 kb on + strand, within PP_0149at 158.770 kb on + strand, within PP_0149at 158.771 kb on - strand, within PP_0149at 158.880 kb on - strand, within PP_0149at 158.937 kb on + strand, within PP_0149at 159.041 kb on + strandat 159.041 kb on + strand, within PP_0149at 159.042 kb on - strand, within PP_0149at 159.042 kb on - strand, within PP_0149at 159.042 kb on - strand, within PP_0149at 159.042 kb on - strand, within PP_0149at 159.193 kb on + strand, within PP_0149at 159.193 kb on + strand, within PP_0149at 159.193 kb on + strand, within PP_0149at 159.193 kb on + strand, within PP_0149at 159.194 kb on - strand, within PP_0149at 159.194 kb on - strand, within PP_0149at 159.194 kb on - strand, within PP_0149at 159.194 kb on - strand, within PP_0149at 159.194 kb on - strand, within PP_0149at 159.194 kb on - strand, within PP_0149at 159.295 kb on + strand, within PP_0149at 159.295 kb on + strand, within PP_0149at 159.296 kb on - strand, within PP_0149at 159.343 kb on + strand, within PP_0149at 159.536 kb on - strand, within PP_0149at 159.547 kb on + strand, within PP_0149at 159.548 kb on - strand, within PP_0149at 159.548 kb on - strand, within PP_0149at 159.564 kb on + strand, within PP_0149at 159.564 kb on + strand, within PP_0149at 159.573 kb on + strand, within PP_0149at 159.671 kb on - strand, within PP_0149at 159.676 kb on + strand, within PP_0149at 159.677 kb on - strand, within PP_0149at 159.677 kb on - strand, within PP_0149at 159.677 kb on - strand, within PP_0149at 159.700 kb on + strand, within PP_0149at 159.700 kb on + strand, within PP_0149at 159.701 kb on - strand, within PP_0149at 159.721 kb on + strand, within PP_0149at 159.722 kb on - strand, within PP_0149at 159.742 kb on + strand, within PP_0149at 159.743 kb on - strand, within PP_0149at 159.743 kb on - strand, within PP_0149at 159.766 kb on + strand, within PP_0149at 159.766 kb on + strand, within PP_0149at 159.766 kb on + strand, within PP_0149at 159.766 kb on + strand, within PP_0149at 159.767 kb on - strand, within PP_0149at 159.767 kb on - strand, within PP_0149at 159.767 kb on - strand, within PP_0149at 159.767 kb on - strand, within PP_0149at 159.767 kb on - strand, within PP_0149at 159.829 kb on + strand, within PP_0149at 159.829 kb on + strand, within PP_0149at 159.829 kb on + strand, within PP_0149at 159.829 kb on + strand, within PP_0149at 159.830 kb on - strand, within PP_0149at 159.830 kb on - strand, within PP_0149at 159.830 kb on - strand, within PP_0149at 159.830 kb on - strand, within PP_0149at 160.003 kb on + strand, within PP_0149at 160.004 kb on - strand, within PP_0149at 160.140 kb on + strand, within PP_0149at 160.140 kb on + strand, within PP_0149at 160.159 kb on + strand, within PP_0149at 160.274 kb on + strandat 160.275 kb on - strandat 160.397 kb on + strandat 160.413 kb on - strandat 160.459 kb on + strand, within PP_0150at 160.460 kb on - strand, within PP_0150at 160.460 kb on - strand, within PP_0150at 160.481 kb on + strand, within PP_0150at 160.739 kb on + strandat 160.739 kb on + strandat 160.740 kb on - strandat 160.740 kb on - strandat 160.740 kb on - strandat 160.740 kb on - strandat 160.740 kb on - strandat 160.743 kb on + strandat 160.743 kb on + strandat 160.744 kb on - strandat 160.744 kb on - strandat 160.808 kb on + strand, within PP_0151at 160.809 kb on - strand, within PP_0151at 160.894 kb on + strand, within PP_0151at 160.894 kb on + strand, within PP_0151at 160.894 kb on + strand, within PP_0151at 160.941 kb on + strand, within PP_0151at 160.942 kb on - strand, within PP_0151at 161.029 kb on - strandat 161.351 kb on - strand, within PP_0152

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
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157,520 + PP_0147 0.84 -1.6
157,521 - PP_0147 0.84 -3.0
157,577 - PP_0147 0.89 +1.7
157,682 + -0.6
157,682 + +2.3
157,682 + -2.3
157,682 + +2.3
157,683 - -1.4
157,731 + +0.7
157,731 + -2.5
157,731 + -0.1
157,731 + -1.2
157,732 - +0.7
157,817 + +1.9
157,839 + -1.3
157,839 + -1.3
157,839 + -0.2
157,839 + +1.0
157,839 + +0.4
157,839 + +0.2
157,839 + +0.3
157,840 - -3.1
157,840 - -1.2
157,840 - +1.9
157,840 - -2.2
157,840 - -1.6
157,922 - -0.9
157,936 + +0.2
157,936 + -0.1
157,936 + -3.0
157,987 + -1.1
157,987 + -1.8
157,988 - -0.0
158,004 - +0.8
158,004 - -1.3
158,175 + PP_0148 0.46 +0.3
158,176 + PP_0148 0.46 -1.4
158,176 + PP_0148 0.46 +0.4
158,176 + PP_0148 0.46 -0.3
158,176 + PP_0148 0.46 +0.7
158,176 + PP_0148 0.46 -2.1
158,176 + PP_0148 0.46 -0.4
158,176 + PP_0148 0.46 -1.5
158,177 - PP_0148 0.46 -1.9
158,177 - PP_0148 0.46 -0.1
158,177 - PP_0148 0.46 +0.4
158,177 - PP_0148 0.46 +0.6
158,203 + PP_0148 0.53 +1.0
158,251 - PP_0148 0.64 -1.8
158,304 - PP_0148 0.78 -1.4
158,319 + PP_0148 0.81 -2.6
158,319 + +1.7
158,319 + PP_0148 0.81 -3.0
158,319 + PP_0148 0.81 -0.8
158,319 + PP_0148 0.81 -1.4
158,320 - PP_0148 0.81 +0.2
158,320 - PP_0148 0.81 -1.0
158,320 - PP_0148 0.81 -0.7
158,320 - PP_0148 0.81 +0.8
158,320 - PP_0148 0.81 +0.7
158,393 - -1.0
158,429 + -1.1
158,429 + +0.9
158,429 + -0.4
158,429 + +0.4
158,429 + -0.5
158,430 - +1.6
158,430 - -2.2
158,430 - -1.6
158,430 - -0.6
158,430 - -2.1
158,430 - +0.8
158,430 - +1.4
158,430 - +0.2
158,430 - -1.1
158,430 - -1.8
158,430 - +0.6
158,770 + PP_0149 0.17 -0.1
158,770 + PP_0149 0.17 -1.0
158,771 - PP_0149 0.17 +0.6
158,880 - PP_0149 0.23 -1.5
158,937 + PP_0149 0.26 +0.2
159,041 + +1.1
159,041 + PP_0149 0.31 -2.2
159,042 - PP_0149 0.31 -0.0
159,042 - PP_0149 0.31 -2.2
159,042 - PP_0149 0.31 -0.4
159,042 - PP_0149 0.31 +1.2
159,193 + PP_0149 0.39 -0.3
159,193 + PP_0149 0.39 +0.9
159,193 + PP_0149 0.39 -0.3
159,193 + PP_0149 0.39 +1.4
159,194 - PP_0149 0.39 -0.6
159,194 - PP_0149 0.39 -1.1
159,194 - PP_0149 0.39 -2.2
159,194 - PP_0149 0.39 -0.7
159,194 - PP_0149 0.39 +0.0
159,194 - PP_0149 0.39 +0.4
159,295 + PP_0149 0.44 +1.0
159,295 + PP_0149 0.44 +2.8
159,296 - PP_0149 0.44 +0.3
159,343 + PP_0149 0.47 -0.9
159,536 - PP_0149 0.56 -0.8
159,547 + PP_0149 0.57 +1.1
159,548 - PP_0149 0.57 -0.4
159,548 - PP_0149 0.57 -0.3
159,564 + PP_0149 0.58 -2.8
159,564 + PP_0149 0.58 -0.3
159,573 + PP_0149 0.58 -1.5
159,671 - PP_0149 0.63 -0.8
159,676 + PP_0149 0.64 -0.1
159,677 - PP_0149 0.64 -2.5
159,677 - PP_0149 0.64 -1.5
159,677 - PP_0149 0.64 +1.1
159,700 + PP_0149 0.65 -1.0
159,700 + PP_0149 0.65 -2.8
159,701 - PP_0149 0.65 +0.4
159,721 + PP_0149 0.66 +2.4
159,722 - PP_0149 0.66 -0.3
159,742 + PP_0149 0.67 -1.2
159,743 - PP_0149 0.67 -0.3
159,743 - PP_0149 0.67 -0.8
159,766 + PP_0149 0.68 -3.1
159,766 + PP_0149 0.68 +0.7
159,766 + PP_0149 0.68 +1.3
159,766 + PP_0149 0.68 -2.0
159,767 - PP_0149 0.68 +1.6
159,767 - PP_0149 0.68 +1.3
159,767 - PP_0149 0.68 -1.9
159,767 - PP_0149 0.68 -1.0
159,767 - PP_0149 0.68 -0.2
159,829 + PP_0149 0.71 -1.8
159,829 + PP_0149 0.71 -0.9
159,829 + PP_0149 0.71 -0.9
159,829 + PP_0149 0.71 -1.1
159,830 - PP_0149 0.71 +1.3
159,830 - PP_0149 0.71 +0.0
159,830 - PP_0149 0.71 -0.9
159,830 - PP_0149 0.71 -3.0
160,003 + PP_0149 0.80 -1.8
160,004 - PP_0149 0.80 -0.3
160,140 + PP_0149 0.87 -0.9
160,140 + PP_0149 0.87 -1.2
160,159 + PP_0149 0.88 -1.3
160,274 + +0.8
160,275 - -1.7
160,397 + +2.2
160,413 - -0.3
160,459 + PP_0150 0.23 -0.3
160,460 - PP_0150 0.23 +0.9
160,460 - PP_0150 0.23 +1.0
160,481 + PP_0150 0.30 -2.0
160,739 + -0.9
160,739 + +1.5
160,740 - -2.5
160,740 - +0.5
160,740 - +0.8
160,740 - +0.8
160,740 - -0.1
160,743 + -0.0
160,743 + +0.6
160,744 - -0.2
160,744 - -3.0
160,808 + PP_0151 0.22 +2.1
160,809 - PP_0151 0.23 +1.1
160,894 + PP_0151 0.52 +0.7
160,894 + PP_0151 0.52 +0.8
160,894 + PP_0151 0.52 +2.6
160,941 + PP_0151 0.68 -2.0
160,942 - PP_0151 0.68 -2.2
161,029 - -1.1
161,351 - PP_0152 0.59 +3.0

Or see this region's nucleotide sequence