Experiment: P. simiae OR Antrim MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19585 and PS417_19590 overlap by 4 nucleotides PS417_19590 and PS417_19595 are separated by 117 nucleotides PS417_19595 and PS417_19600 are separated by 73 nucleotides PS417_19600 and PS417_19605 are separated by 187 nucleotides
PS417_19585: PS417_19585 - acyl-CoA dehydrogenase, at 4,260,043 to 4,261,200
_19585
PS417_19590: PS417_19590 - enoyl-CoA hydratase, at 4,261,197 to 4,261,958
_19590
PS417_19595: PS417_19595 - 3-methylcrotonyl-CoA carboxylase, at 4,262,076 to 4,263,959
_19595
PS417_19600: PS417_19600 - exonuclease, at 4,264,033 to 4,264,575
_19600
PS417_19605: PS417_19605 - hypothetical protein, at 4,264,763 to 4,265,047
_19605
Position (kb)
4262
4263
4264 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4261.414 kb on + strand, within PS417_19590 at 4261.414 kb on + strand, within PS417_19590 at 4261.490 kb on - strand, within PS417_19590 at 4261.595 kb on - strand, within PS417_19590 at 4261.952 kb on + strand at 4262.069 kb on + strand at 4262.176 kb on - strand at 4262.206 kb on + strand at 4262.206 kb on + strand at 4262.259 kb on + strand at 4262.259 kb on + strand at 4262.260 kb on - strand at 4262.260 kb on - strand at 4262.883 kb on + strand, within PS417_19595 at 4262.883 kb on + strand, within PS417_19595 at 4262.883 kb on + strand, within PS417_19595 at 4262.884 kb on - strand, within PS417_19595 at 4262.934 kb on + strand, within PS417_19595 at 4263.219 kb on + strand, within PS417_19595 at 4263.302 kb on + strand, within PS417_19595 at 4263.303 kb on - strand, within PS417_19595 at 4263.396 kb on - strand, within PS417_19595 at 4263.472 kb on - strand, within PS417_19595 at 4263.472 kb on - strand, within PS417_19595 at 4263.685 kb on + strand, within PS417_19595 at 4263.907 kb on + strand at 4263.907 kb on + strand at 4263.908 kb on - strand at 4263.908 kb on - strand at 4263.965 kb on - strand at 4263.975 kb on - strand at 4263.995 kb on - strand at 4264.018 kb on - strand at 4264.051 kb on - strand at 4264.339 kb on + strand, within PS417_19600 at 4264.340 kb on - strand, within PS417_19600 at 4264.573 kb on + strand at 4264.599 kb on - strand at 4264.625 kb on - strand at 4264.625 kb on - strand at 4264.631 kb on - strand at 4264.725 kb on - strand at 4264.729 kb on + strand at 4264.729 kb on + strand at 4264.730 kb on - strand at 4264.730 kb on - strand at 4264.782 kb on - strand at 4264.782 kb on - strand at 4264.782 kb on - strand at 4264.862 kb on + strand, within PS417_19605 at 4264.863 kb on - strand, within PS417_19605
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae OR Antrim MOI 1 remove 4,261,414 + PS417_19590 0.28 +0.4 4,261,414 + PS417_19590 0.28 -0.6 4,261,490 - PS417_19590 0.38 +1.7 4,261,595 - PS417_19590 0.52 -0.5 4,261,952 + -1.3 4,262,069 + -2.2 4,262,176 - -1.3 4,262,206 + +1.1 4,262,206 + +1.3 4,262,259 + +0.8 4,262,259 + -0.1 4,262,260 - -0.1 4,262,260 - +1.5 4,262,883 + PS417_19595 0.43 +0.3 4,262,883 + PS417_19595 0.43 +1.2 4,262,883 + PS417_19595 0.43 +0.2 4,262,884 - PS417_19595 0.43 -0.7 4,262,934 + PS417_19595 0.46 -0.8 4,263,219 + PS417_19595 0.61 +1.0 4,263,302 + PS417_19595 0.65 -1.7 4,263,303 - PS417_19595 0.65 -1.4 4,263,396 - PS417_19595 0.70 -0.8 4,263,472 - PS417_19595 0.74 -0.4 4,263,472 - PS417_19595 0.74 -0.5 4,263,685 + PS417_19595 0.85 -1.2 4,263,907 + -1.2 4,263,907 + -0.6 4,263,908 - -1.3 4,263,908 - -0.1 4,263,965 - -0.9 4,263,975 - +1.2 4,263,995 - -1.3 4,264,018 - -1.8 4,264,051 - -1.6 4,264,339 + PS417_19600 0.56 -1.7 4,264,340 - PS417_19600 0.57 -2.1 4,264,573 + -0.4 4,264,599 - +0.1 4,264,625 - -0.8 4,264,625 - -0.5 4,264,631 - +1.5 4,264,725 - -0.4 4,264,729 + -2.0 4,264,729 + -1.8 4,264,730 - -1.0 4,264,730 - -0.1 4,264,782 - -3.2 4,264,782 - -0.4 4,264,782 - -0.5 4,264,862 + PS417_19605 0.35 -0.9 4,264,863 - PS417_19605 0.35 -1.3
Or see this region's nucleotide sequence