Experiment: P. simiae OR Antrim MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13840 and PS417_13845 are separated by 61 nucleotides PS417_13845 and PS417_13850 are separated by 11 nucleotides PS417_13850 and PS417_13855 are separated by 9 nucleotides
PS417_13840: PS417_13840 - isobutyryl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 3,040,452 to 3,041,603
_13840
PS417_13845: PS417_13845 - enoyl-CoA hydratase, at 3,041,665 to 3,042,438
_13845
PS417_13850: PS417_13850 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 3,042,450 to 3,043,577
_13850
PS417_13855: PS417_13855 - acetyl-CoA acetyltransferase, at 3,043,587 to 3,044,771
_13855
Position (kb)
3042
3043
3044 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3041.481 kb on + strand, within PS417_13840 at 3041.482 kb on - strand, within PS417_13840 at 3041.482 kb on - strand, within PS417_13840 at 3041.614 kb on + strand at 3043.006 kb on + strand at 3043.137 kb on + strand, within PS417_13850 at 3043.138 kb on - strand, within PS417_13850 at 3043.141 kb on + strand, within PS417_13850 at 3043.141 kb on + strand, within PS417_13850 at 3043.142 kb on - strand, within PS417_13850 at 3043.142 kb on - strand, within PS417_13850 at 3043.178 kb on + strand, within PS417_13850 at 3043.378 kb on + strand, within PS417_13850 at 3043.591 kb on + strand at 3043.592 kb on - strand at 3044.401 kb on + strand, within PS417_13855
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae OR Antrim MOI 1 remove 3,041,481 + PS417_13840 0.89 -2.0 3,041,482 - PS417_13840 0.89 +1.4 3,041,482 - PS417_13840 0.89 +0.9 3,041,614 + -0.4 3,043,006 + -0.9 3,043,137 + PS417_13850 0.61 +0.5 3,043,138 - PS417_13850 0.61 +0.1 3,043,141 + PS417_13850 0.61 +0.4 3,043,141 + PS417_13850 0.61 +0.9 3,043,142 - PS417_13850 0.61 +0.8 3,043,142 - PS417_13850 0.61 +1.1 3,043,178 + PS417_13850 0.65 +1.9 3,043,378 + PS417_13850 0.82 +0.6 3,043,591 + -1.9 3,043,592 - +0.8 3,044,401 + PS417_13855 0.69 -0.2
Or see this region's nucleotide sequence