Experiment: P. simiae OR Antrim MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13000 and PS417_13005 are separated by 13 nucleotides PS417_13005 and PS417_13010 are separated by 14 nucleotides PS417_13010 and PS417_13015 overlap by 4 nucleotides
PS417_13000: PS417_13000 - isochorismatase, at 2,840,542 to 2,841,174
_13000
PS417_13005: PS417_13005 - Asp/Glu racemase, at 2,841,188 to 2,841,940
_13005
PS417_13010: PS417_13010 - alpha/beta hydrolase, at 2,841,955 to 2,842,752
_13010
PS417_13015: PS417_13015 - 6-hydroxynicotinate 3-monooxygenase, at 2,842,749 to 2,843,882
_13015
Position (kb)
2841
2842
2843 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2841.034 kb on + strand at 2841.192 kb on + strand at 2841.498 kb on + strand, within PS417_13005 at 2841.498 kb on + strand, within PS417_13005 at 2841.499 kb on - strand, within PS417_13005 at 2841.794 kb on + strand, within PS417_13005 at 2841.956 kb on + strand at 2842.326 kb on - strand, within PS417_13010 at 2842.469 kb on + strand, within PS417_13010 at 2842.470 kb on - strand, within PS417_13010 at 2842.792 kb on + strand at 2842.798 kb on + strand at 2842.892 kb on - strand, within PS417_13015 at 2843.221 kb on + strand, within PS417_13015 at 2843.222 kb on - strand, within PS417_13015 at 2843.222 kb on - strand, within PS417_13015 at 2843.224 kb on + strand, within PS417_13015 at 2843.224 kb on + strand, within PS417_13015 at 2843.224 kb on + strand, within PS417_13015 at 2843.227 kb on + strand, within PS417_13015 at 2843.228 kb on - strand, within PS417_13015 at 2843.252 kb on - strand, within PS417_13015 at 2843.252 kb on - strand, within PS417_13015 at 2843.252 kb on - strand, within PS417_13015 at 2843.368 kb on + strand, within PS417_13015 at 2843.447 kb on - strand, within PS417_13015 at 2843.590 kb on + strand, within PS417_13015 at 2843.591 kb on - strand, within PS417_13015
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae OR Antrim MOI 1 remove 2,841,034 + +0.4 2,841,192 + -0.7 2,841,498 + PS417_13005 0.41 +0.0 2,841,498 + PS417_13005 0.41 -1.4 2,841,499 - PS417_13005 0.41 -0.8 2,841,794 + PS417_13005 0.80 +0.5 2,841,956 + +0.7 2,842,326 - PS417_13010 0.46 -0.6 2,842,469 + PS417_13010 0.64 -0.8 2,842,470 - PS417_13010 0.65 -0.5 2,842,792 + +1.4 2,842,798 + -0.2 2,842,892 - PS417_13015 0.13 -0.8 2,843,221 + PS417_13015 0.42 +1.4 2,843,222 - PS417_13015 0.42 -1.2 2,843,222 - PS417_13015 0.42 -0.2 2,843,224 + PS417_13015 0.42 -1.2 2,843,224 + PS417_13015 0.42 -0.4 2,843,224 + PS417_13015 0.42 -1.3 2,843,227 + PS417_13015 0.42 +2.8 2,843,228 - PS417_13015 0.42 -3.0 2,843,252 - PS417_13015 0.44 -1.8 2,843,252 - PS417_13015 0.44 +0.4 2,843,252 - PS417_13015 0.44 -0.2 2,843,368 + PS417_13015 0.55 +0.1 2,843,447 - PS417_13015 0.62 +0.3 2,843,590 + PS417_13015 0.74 +0.7 2,843,591 - PS417_13015 0.74 -0.0
Or see this region's nucleotide sequence