Experiment: P. simiae OR Antrim MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_08535 and PS417_08540 are separated by 422 nucleotides PS417_08540 and PS417_08545 are separated by 96 nucleotides PS417_08545 and PS417_08550 are separated by 103 nucleotides
PS417_08535: PS417_08535 - enoyl-CoA hydratase, at 1,864,014 to 1,864,805
_08535
PS417_08540: PS417_08540 - RNA helicase, at 1,865,228 to 1,866,901
_08540
PS417_08545: PS417_08545 - aromatic ring-opening dioxygenase LigB, at 1,866,998 to 1,867,765
_08545
PS417_08550: PS417_08550 - thiopurine S-methyltransferase, at 1,867,869 to 1,868,525
_08550
Position (kb)
1865
1866
1867 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1864.746 kb on + strand at 1864.790 kb on + strand at 1864.871 kb on + strand at 1864.925 kb on - strand at 1864.925 kb on - strand at 1865.004 kb on + strand at 1865.004 kb on + strand at 1865.004 kb on + strand at 1865.036 kb on + strand at 1865.036 kb on + strand at 1865.215 kb on + strand at 1866.704 kb on - strand, within PS417_08540 at 1866.930 kb on - strand at 1866.930 kb on - strand at 1866.980 kb on - strand at 1867.012 kb on - strand at 1867.089 kb on + strand, within PS417_08545 at 1867.410 kb on + strand, within PS417_08545 at 1867.411 kb on - strand, within PS417_08545 at 1867.765 kb on + strand at 1867.765 kb on + strand at 1867.765 kb on + strand at 1867.766 kb on - strand at 1867.766 kb on - strand at 1867.766 kb on - strand at 1867.766 kb on - strand at 1867.825 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae OR Antrim MOI 1 remove 1,864,746 + +0.7 1,864,790 + +0.2 1,864,871 + -0.3 1,864,925 - +0.1 1,864,925 - -0.4 1,865,004 + -0.9 1,865,004 + -1.6 1,865,004 + +2.1 1,865,036 + -0.2 1,865,036 + -0.9 1,865,215 + -1.1 1,866,704 - PS417_08540 0.88 -1.5 1,866,930 - -0.7 1,866,930 - -0.5 1,866,980 - +1.8 1,867,012 - -1.5 1,867,089 + PS417_08545 0.12 -1.0 1,867,410 + PS417_08545 0.54 -0.5 1,867,411 - PS417_08545 0.54 +0.7 1,867,765 + -3.4 1,867,765 + -0.5 1,867,765 + +0.4 1,867,766 - +0.1 1,867,766 - -0.1 1,867,766 - +0.5 1,867,766 - -0.5 1,867,825 - -1.9
Or see this region's nucleotide sequence