Strain Fitness in Pseudomonas simiae WCS417 around PS417_18200

Experiment: P. simiae OR Antrim MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_18195 and PS417_18200 are separated by 22 nucleotidesPS417_18200 and PS417_18205 are separated by 70 nucleotides PS417_18195: PS417_18195 - 3-(3-hydroxyphenyl)propionate hydroxylase, at 3,963,325 to 3,964,971 _18195 PS417_18200: PS417_18200 - glyoxalase, at 3,964,994 to 3,965,842 _18200 PS417_18205: PS417_18205 - aldehyde dehydrogenase, at 3,965,913 to 3,967,391 _18205 Position (kb) 3964 3965 3966Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3964.126 kb on + strand, within PS417_18195at 3964.463 kb on - strand, within PS417_18195at 3964.769 kb on + strand, within PS417_18195at 3964.847 kb on - strandat 3964.847 kb on - strandat 3964.847 kb on - strandat 3965.444 kb on + strand, within PS417_18200at 3965.654 kb on - strand, within PS417_18200at 3965.801 kb on + strandat 3965.802 kb on - strandat 3965.874 kb on + strandat 3965.875 kb on - strandat 3965.902 kb on - strandat 3965.906 kb on + strandat 3965.907 kb on - strandat 3965.937 kb on + strandat 3965.937 kb on + strandat 3965.959 kb on - strandat 3965.959 kb on - strandat 3966.318 kb on + strand, within PS417_18205at 3966.318 kb on + strand, within PS417_18205at 3966.400 kb on - strand, within PS417_18205at 3966.400 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P. simiae OR Antrim MOI 10
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3,964,126 + PS417_18195 0.49 -1.6
3,964,463 - PS417_18195 0.69 +0.3
3,964,769 + PS417_18195 0.88 +0.2
3,964,847 - +0.1
3,964,847 - +2.1
3,964,847 - -0.5
3,965,444 + PS417_18200 0.53 -1.0
3,965,654 - PS417_18200 0.78 -0.3
3,965,801 + -0.7
3,965,802 - -0.3
3,965,874 + -2.0
3,965,875 - -1.3
3,965,902 - -2.5
3,965,906 + -0.9
3,965,907 - +0.1
3,965,937 + -0.0
3,965,937 + +0.8
3,965,959 - +0.1
3,965,959 - +0.4
3,966,318 + PS417_18205 0.27 -0.5
3,966,318 + PS417_18205 0.27 +0.7
3,966,400 - PS417_18205 0.33 +1.5
3,966,400 - +4.1

Or see this region's nucleotide sequence