Experiment: P. simiae OR Antrim MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11265 and PS417_11270 overlap by 14 nucleotides PS417_11270 and PS417_11275 are separated by 148 nucleotides PS417_11275 and PS417_11280 are separated by 8 nucleotides PS417_11280 and PS417_11285 overlap by 4 nucleotides
PS417_11265: PS417_11265 - general secretion pathway protein GspC, at 2,412,995 to 2,413,426
_11265
PS417_11270: PS417_11270 - general secretion pathway protein GspI, at 2,413,413 to 2,413,778
_11270
PS417_11275: PS417_11275 - general secretion pathway protein GspG, at 2,413,927 to 2,414,376
_11275
PS417_11280: PS417_11280 - HxcX atypical pseudopilin, at 2,414,385 to 2,415,254
_11280
PS417_11285: PS417_11285 - general secretion pathway protein GspL, at 2,415,251 to 2,416,312
_11285
Position (kb)
2414
2415
2416 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2413.748 kb on + strand at 2413.774 kb on + strand at 2413.775 kb on - strand at 2413.863 kb on + strand at 2414.129 kb on - strand, within PS417_11275 at 2414.269 kb on + strand, within PS417_11275 at 2414.269 kb on + strand at 2414.345 kb on + strand at 2414.345 kb on + strand at 2414.375 kb on - strand at 2414.770 kb on + strand at 2414.771 kb on - strand, within PS417_11280 at 2414.771 kb on - strand, within PS417_11280 at 2414.919 kb on + strand, within PS417_11280 at 2414.920 kb on - strand, within PS417_11280 at 2414.942 kb on + strand, within PS417_11280 at 2414.943 kb on - strand, within PS417_11280 at 2414.943 kb on - strand, within PS417_11280
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae OR Antrim MOI 10 remove 2,413,748 + -1.2 2,413,774 + -0.9 2,413,775 - +0.8 2,413,863 + -0.8 2,414,129 - PS417_11275 0.45 -2.0 2,414,269 + PS417_11275 0.76 -0.4 2,414,269 + -0.5 2,414,345 + -0.3 2,414,345 + +1.5 2,414,375 - -0.9 2,414,770 + +0.5 2,414,771 - PS417_11280 0.44 +1.9 2,414,771 - PS417_11280 0.44 +0.6 2,414,919 + PS417_11280 0.61 +0.5 2,414,920 - PS417_11280 0.61 +0.7 2,414,942 + PS417_11280 0.64 -3.2 2,414,943 - PS417_11280 0.64 +0.1 2,414,943 - PS417_11280 0.64 -1.2
Or see this region's nucleotide sequence