Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2512 and PP_5535 are separated by 35 nucleotides PP_5535 and PP_2513 are separated by 23 nucleotides PP_2513 and PP_2514 are separated by 2 nucleotides PP_2514 and PP_2515 overlap by 4 nucleotides
PP_2512: PP_2512 - GTP cyclohydrolase 1, at 2,859,168 to 2,859,728
_2512
PP_5535: PP_5535 - conserved membrane protein of unknown function, at 2,859,764 to 2,860,222
_5535
PP_2513: PP_2513 - 4-oxalomesaconate tautomerase, at 2,860,246 to 2,861,331
_2513
PP_2514: PP_2514 - 4-carboxy-4-hydroxy-2-oxoadipic acid aldolase, at 2,861,334 to 2,862,050
_2514
PP_2515: PP_2515 - 4-oxalmesaconate hydratase, at 2,862,047 to 2,862,823
_2515
Position (kb)
2860
2861
2862 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2859.279 kb on + strand, within PP_2512 at 2859.279 kb on + strand, within PP_2512 at 2859.280 kb on - strand, within PP_2512 at 2859.436 kb on - strand, within PP_2512 at 2859.436 kb on - strand, within PP_2512 at 2859.457 kb on + strand, within PP_2512 at 2859.458 kb on - strand, within PP_2512 at 2859.458 kb on - strand, within PP_2512 at 2859.458 kb on - strand, within PP_2512 at 2859.488 kb on + strand, within PP_2512 at 2859.488 kb on + strand, within PP_2512 at 2859.489 kb on - strand, within PP_2512 at 2859.489 kb on - strand, within PP_2512 at 2859.506 kb on - strand, within PP_2512 at 2859.512 kb on - strand, within PP_2512 at 2859.599 kb on + strand, within PP_2512 at 2859.599 kb on + strand, within PP_2512 at 2859.599 kb on + strand, within PP_2512 at 2859.599 kb on + strand, within PP_2512 at 2859.600 kb on - strand, within PP_2512 at 2859.600 kb on - strand, within PP_2512 at 2859.600 kb on - strand, within PP_2512 at 2859.600 kb on - strand, within PP_2512 at 2859.600 kb on - strand, within PP_2512 at 2859.661 kb on + strand, within PP_2512 at 2859.778 kb on + strand at 2859.779 kb on - strand at 2859.779 kb on - strand at 2859.787 kb on - strand at 2859.787 kb on - strand at 2859.815 kb on + strand, within PP_5535 at 2859.836 kb on - strand, within PP_5535 at 2860.012 kb on + strand, within PP_5535 at 2860.012 kb on + strand, within PP_5535 at 2860.012 kb on + strand, within PP_5535 at 2860.013 kb on - strand, within PP_5535 at 2860.014 kb on + strand, within PP_5535 at 2860.015 kb on - strand, within PP_5535 at 2860.015 kb on - strand, within PP_5535 at 2860.015 kb on - strand, within PP_5535 at 2860.139 kb on + strand, within PP_5535 at 2860.139 kb on + strand, within PP_5535 at 2860.237 kb on + strand at 2860.269 kb on + strand at 2860.269 kb on + strand at 2860.467 kb on + strand at 2860.468 kb on - strand at 2860.898 kb on + strand, within PP_2513 at 2860.898 kb on + strand, within PP_2513 at 2860.899 kb on - strand, within PP_2513 at 2861.060 kb on + strand, within PP_2513 at 2861.061 kb on - strand, within PP_2513 at 2861.102 kb on + strand, within PP_2513 at 2861.102 kb on + strand, within PP_2513 at 2861.314 kb on + strand at 2861.365 kb on + strand at 2861.366 kb on - strand at 2861.404 kb on + strand at 2861.404 kb on + strand at 2861.404 kb on + strand at 2861.405 kb on - strand at 2861.405 kb on - strand at 2861.618 kb on + strand, within PP_2514 at 2861.619 kb on - strand, within PP_2514 at 2861.619 kb on - strand, within PP_2514 at 2861.793 kb on + strand, within PP_2514 at 2861.794 kb on - strand, within PP_2514 at 2861.794 kb on - strand, within PP_2514 at 2861.916 kb on - strand, within PP_2514 at 2862.008 kb on + strand at 2862.008 kb on + strand at 2862.011 kb on + strand at 2862.011 kb on + strand at 2862.012 kb on - strand at 2862.012 kb on - strand at 2862.012 kb on - strand at 2862.012 kb on - strand at 2862.134 kb on - strand, within PP_2515 at 2862.165 kb on - strand, within PP_2515 at 2862.174 kb on + strand, within PP_2515 at 2862.174 kb on + strand, within PP_2515 at 2862.174 kb on + strand, within PP_2515 at 2862.174 kb on + strand, within PP_2515 at 2862.174 kb on + strand, within PP_2515 at 2862.175 kb on - strand, within PP_2515 at 2862.175 kb on - strand, within PP_2515 at 2862.175 kb on - strand, within PP_2515 at 2862.177 kb on + strand, within PP_2515 at 2862.177 kb on + strand, within PP_2515 at 2862.178 kb on - strand, within PP_2515 at 2862.178 kb on - strand, within PP_2515 at 2862.222 kb on + strand, within PP_2515 at 2862.223 kb on - strand, within PP_2515 at 2862.244 kb on + strand, within PP_2515 at 2862.244 kb on + strand, within PP_2515 at 2862.245 kb on - strand, within PP_2515 at 2862.306 kb on + strand, within PP_2515
Per-strain Table
Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs remove 2,859,279 + PP_2512 0.20 -2.9 2,859,279 + PP_2512 0.20 +0.7 2,859,280 - PP_2512 0.20 -0.2 2,859,436 - PP_2512 0.48 -0.3 2,859,436 - PP_2512 0.48 -0.1 2,859,457 + PP_2512 0.52 -1.9 2,859,458 - PP_2512 0.52 +0.1 2,859,458 - PP_2512 0.52 -0.9 2,859,458 - PP_2512 0.52 -0.0 2,859,488 + PP_2512 0.57 +0.6 2,859,488 + PP_2512 0.57 +0.8 2,859,489 - PP_2512 0.57 -0.5 2,859,489 - PP_2512 0.57 -0.8 2,859,506 - PP_2512 0.60 +0.1 2,859,512 - PP_2512 0.61 +0.1 2,859,599 + PP_2512 0.77 -0.5 2,859,599 + PP_2512 0.77 -2.1 2,859,599 + PP_2512 0.77 -0.7 2,859,599 + PP_2512 0.77 -0.1 2,859,600 - PP_2512 0.77 +0.6 2,859,600 - PP_2512 0.77 +0.5 2,859,600 - PP_2512 0.77 +1.6 2,859,600 - PP_2512 0.77 -3.0 2,859,600 - PP_2512 0.77 -0.4 2,859,661 + PP_2512 0.88 -0.4 2,859,778 + +0.9 2,859,779 - -0.1 2,859,779 - +0.5 2,859,787 - +0.4 2,859,787 - +1.1 2,859,815 + PP_5535 0.11 +0.2 2,859,836 - PP_5535 0.16 +0.9 2,860,012 + PP_5535 0.54 -1.1 2,860,012 + PP_5535 0.54 +1.4 2,860,012 + PP_5535 0.54 +0.4 2,860,013 - PP_5535 0.54 +1.8 2,860,014 + PP_5535 0.54 +0.7 2,860,015 - PP_5535 0.55 -1.1 2,860,015 - PP_5535 0.55 -0.3 2,860,015 - PP_5535 0.55 -1.6 2,860,139 + PP_5535 0.82 +0.7 2,860,139 + PP_5535 0.82 -0.9 2,860,237 + -3.0 2,860,269 + -3.2 2,860,269 + -3.1 2,860,467 + -0.4 2,860,468 - -0.9 2,860,898 + PP_2513 0.60 -4.1 2,860,898 + PP_2513 0.60 +0.8 2,860,899 - PP_2513 0.60 -3.9 2,861,060 + PP_2513 0.75 -3.0 2,861,061 - PP_2513 0.75 +0.9 2,861,102 + PP_2513 0.79 +2.5 2,861,102 + PP_2513 0.79 +0.3 2,861,314 + -0.9 2,861,365 + -0.8 2,861,366 - -0.7 2,861,404 + +0.4 2,861,404 + +0.1 2,861,404 + +0.4 2,861,405 - -1.5 2,861,405 - -0.2 2,861,618 + PP_2514 0.40 -0.9 2,861,619 - PP_2514 0.40 +0.6 2,861,619 - PP_2514 0.40 -2.9 2,861,793 + PP_2514 0.64 -0.0 2,861,794 - PP_2514 0.64 -1.9 2,861,794 - PP_2514 0.64 +0.2 2,861,916 - PP_2514 0.81 -0.1 2,862,008 + +0.6 2,862,008 + -2.5 2,862,011 + +0.0 2,862,011 + +0.1 2,862,012 - +0.2 2,862,012 - +0.6 2,862,012 - -0.4 2,862,012 - -3.6 2,862,134 - PP_2515 0.11 -0.8 2,862,165 - PP_2515 0.15 -0.4 2,862,174 + PP_2515 0.16 +0.3 2,862,174 + PP_2515 0.16 -0.9 2,862,174 + PP_2515 0.16 -0.6 2,862,174 + PP_2515 0.16 +0.5 2,862,174 + PP_2515 0.16 +1.4 2,862,175 - PP_2515 0.16 +0.1 2,862,175 - PP_2515 0.16 +1.2 2,862,175 - PP_2515 0.16 +0.2 2,862,177 + PP_2515 0.17 -2.0 2,862,177 + PP_2515 0.17 +0.1 2,862,178 - PP_2515 0.17 -0.3 2,862,178 - PP_2515 0.17 +0.6 2,862,222 + PP_2515 0.23 +1.0 2,862,223 - PP_2515 0.23 +0.0 2,862,244 + PP_2515 0.25 +1.2 2,862,244 + PP_2515 0.25 -0.6 2,862,245 - PP_2515 0.25 +1.1 2,862,306 + PP_2515 0.33 +0.5
Or see this region's nucleotide sequence