Strain Fitness in Pseudomonas putida KT2440 around PP_0241

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0239 and PP_0240 overlap by 4 nucleotidesPP_0240 and PP_0241 are separated by 27 nucleotidesPP_0241 and PP_0242 are separated by 141 nucleotidesPP_0242 and PP_0243 overlap by 1 nucleotides PP_0239: PP_0239 - aliphatic sulfonate ABC transporter - permease subunit / transport of isethionate, at 293,803 to 294,600 _0239 PP_0240: PP_0240 - Aliphatic sulfonates import ATP-binding protein SsuB, at 294,597 to 295,409 _0240 PP_0241: PP_0241 - Transporter, at 295,437 to 295,652 _0241 PP_0242: PP_0242 - Transcriptional regulator, TetR family, at 295,794 to 296,426 _0242 PP_0243: PP_0243 - Glutamate--cysteine ligase, at 296,426 to 298,003 _0243 Position (kb) 295 296Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 294.476 kb on + strand, within PP_0239at 294.496 kb on + strand, within PP_0239at 294.496 kb on + strand, within PP_0239at 294.497 kb on - strand, within PP_0239at 294.572 kb on - strandat 294.572 kb on - strandat 295.168 kb on + strand, within PP_0240at 295.168 kb on + strand, within PP_0240at 295.169 kb on - strand, within PP_0240at 295.169 kb on - strand, within PP_0240at 295.372 kb on + strandat 295.373 kb on - strandat 295.442 kb on + strandat 295.443 kb on - strandat 295.443 kb on - strandat 295.443 kb on - strandat 295.478 kb on + strand, within PP_0241at 295.479 kb on - strand, within PP_0241at 295.559 kb on + strand, within PP_0241at 295.560 kb on - strand, within PP_0241at 295.637 kb on + strandat 295.637 kb on + strandat 295.828 kb on + strandat 295.829 kb on - strandat 295.829 kb on - strandat 295.840 kb on + strandat 295.841 kb on - strandat 295.841 kb on - strandat 295.915 kb on + strand, within PP_0242at 295.915 kb on + strand, within PP_0242at 295.915 kb on + strand, within PP_0242at 295.915 kb on + strand, within PP_0242at 295.915 kb on + strand, within PP_0242at 295.916 kb on - strand, within PP_0242at 295.916 kb on - strand, within PP_0242at 295.916 kb on - strand, within PP_0242at 295.916 kb on - strand, within PP_0242at 295.916 kb on - strand, within PP_0242at 295.916 kb on - strand, within PP_0242at 296.053 kb on - strand, within PP_0242at 296.110 kb on + strand, within PP_0242at 296.110 kb on + strand, within PP_0242at 296.111 kb on - strand, within PP_0242at 296.111 kb on - strand, within PP_0242at 296.114 kb on + strand, within PP_0242at 296.115 kb on - strand, within PP_0242at 296.115 kb on - strand, within PP_0242at 296.225 kb on + strand, within PP_0242at 296.225 kb on + strand, within PP_0242at 296.226 kb on - strand, within PP_0242at 296.226 kb on - strand, within PP_0242at 296.308 kb on + strand, within PP_0242at 296.308 kb on + strand, within PP_0242at 296.312 kb on + strand, within PP_0242at 296.458 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
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294,476 + PP_0239 0.84 -0.9
294,496 + PP_0239 0.87 -0.1
294,496 + PP_0239 0.87 -1.0
294,497 - PP_0239 0.87 -1.8
294,572 - -1.2
294,572 - -0.3
295,168 + PP_0240 0.70 +1.2
295,168 + PP_0240 0.70 -3.7
295,169 - PP_0240 0.70 +0.4
295,169 - PP_0240 0.70 -1.2
295,372 + -2.2
295,373 - +1.0
295,442 + -1.2
295,443 - -0.0
295,443 - +0.2
295,443 - +0.6
295,478 + PP_0241 0.19 +1.3
295,479 - PP_0241 0.19 +1.4
295,559 + PP_0241 0.56 -2.0
295,560 - PP_0241 0.57 +1.5
295,637 + -2.3
295,637 + -0.8
295,828 + +0.4
295,829 - +0.8
295,829 - -1.1
295,840 + -4.2
295,841 - -0.7
295,841 - +0.3
295,915 + PP_0242 0.19 -0.2
295,915 + PP_0242 0.19 -1.7
295,915 + PP_0242 0.19 +0.1
295,915 + PP_0242 0.19 +0.1
295,915 + PP_0242 0.19 +1.3
295,916 - PP_0242 0.19 -3.7
295,916 - PP_0242 0.19 -0.1
295,916 - PP_0242 0.19 +0.3
295,916 - PP_0242 0.19 +0.3
295,916 - PP_0242 0.19 +0.8
295,916 - PP_0242 0.19 +0.4
296,053 - PP_0242 0.41 +1.1
296,110 + PP_0242 0.50 +0.6
296,110 + PP_0242 0.50 -1.5
296,111 - PP_0242 0.50 -0.8
296,111 - PP_0242 0.50 +0.4
296,114 + PP_0242 0.51 -1.5
296,115 - PP_0242 0.51 -1.0
296,115 - PP_0242 0.51 +0.2
296,225 + PP_0242 0.68 +0.5
296,225 + PP_0242 0.68 +0.2
296,226 - PP_0242 0.68 -2.6
296,226 - PP_0242 0.68 -0.5
296,308 + PP_0242 0.81 +0.3
296,308 + PP_0242 0.81 -1.9
296,312 + PP_0242 0.82 -0.7
296,458 - -0.6

Or see this region's nucleotide sequence