Strain Fitness in Pseudomonas putida KT2440 around PP_0076

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0075 and PP_0076 are separated by 144 nucleotidesPP_0076 and PP_0077 are separated by 14 nucleotides PP_0075: PP_0075 - putative choline sulfate transporter, at 85,152 to 86,717 _0075 PP_0076: PP_0076 - putative choline betaine-binding protein, at 86,862 to 87,785 _0076 PP_0077: PP_0077 - choline-sulfatase, at 87,800 to 89,317 _0077 Position (kb) 86 87 88Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 86.031 kb on + strand, within PP_0075at 86.088 kb on + strand, within PP_0075at 86.088 kb on + strand, within PP_0075at 86.088 kb on + strand, within PP_0075at 86.089 kb on - strand, within PP_0075at 86.089 kb on - strand, within PP_0075at 86.089 kb on - strand, within PP_0075at 86.172 kb on + strand, within PP_0075at 86.172 kb on + strand, within PP_0075at 86.172 kb on + strand, within PP_0075at 86.262 kb on + strand, within PP_0075at 86.262 kb on + strand, within PP_0075at 86.263 kb on - strand, within PP_0075at 86.416 kb on + strand, within PP_0075at 86.417 kb on - strand, within PP_0075at 86.501 kb on - strand, within PP_0075at 86.501 kb on - strand, within PP_0075at 86.551 kb on + strand, within PP_0075at 86.634 kb on + strandat 86.634 kb on + strandat 86.634 kb on + strandat 86.635 kb on - strandat 86.700 kb on + strandat 86.701 kb on - strandat 86.863 kb on + strandat 86.864 kb on - strandat 86.864 kb on - strandat 86.998 kb on - strand, within PP_0076at 86.998 kb on - strand, within PP_0076at 86.998 kb on - strand, within PP_0076at 86.998 kb on - strand, within PP_0076at 86.998 kb on - strand, within PP_0076at 87.106 kb on + strand, within PP_0076at 87.107 kb on - strand, within PP_0076at 87.107 kb on - strand, within PP_0076at 87.107 kb on - strand, within PP_0076at 87.107 kb on - strand, within PP_0076at 87.107 kb on - strand, within PP_0076at 87.107 kb on - strand, within PP_0076at 87.107 kb on - strand, within PP_0076at 87.107 kb on - strand, within PP_0076at 87.108 kb on + strand, within PP_0076at 87.109 kb on - strand, within PP_0076at 87.109 kb on - strand, within PP_0076at 87.109 kb on - strand, within PP_0076at 87.151 kb on + strand, within PP_0076at 87.151 kb on + strand, within PP_0076at 87.152 kb on - strand, within PP_0076at 87.427 kb on + strand, within PP_0076at 87.427 kb on + strand, within PP_0076at 87.427 kb on + strand, within PP_0076at 87.427 kb on + strand, within PP_0076at 87.428 kb on - strand, within PP_0076at 87.428 kb on - strand, within PP_0076at 87.428 kb on - strand, within PP_0076at 87.580 kb on + strand, within PP_0076at 87.580 kb on + strand, within PP_0076at 87.580 kb on + strand, within PP_0076at 87.581 kb on - strand, within PP_0076at 87.636 kb on + strand, within PP_0076at 87.669 kb on - strandat 87.669 kb on - strand, within PP_0076at 87.873 kb on + strandat 87.873 kb on + strandat 87.873 kb on + strandat 87.873 kb on + strandat 87.873 kb on + strandat 87.873 kb on + strandat 87.873 kb on + strandat 87.874 kb on - strandat 87.874 kb on - strandat 87.874 kb on - strandat 87.874 kb on - strandat 87.874 kb on - strandat 87.974 kb on + strand, within PP_0077at 87.974 kb on + strand, within PP_0077at 87.975 kb on - strand, within PP_0077at 88.101 kb on + strand, within PP_0077at 88.102 kb on - strand, within PP_0077at 88.102 kb on - strand, within PP_0077at 88.102 kb on - strand, within PP_0077at 88.102 kb on - strand, within PP_0077at 88.106 kb on + strand, within PP_0077at 88.106 kb on + strand, within PP_0077at 88.106 kb on + strand, within PP_0077at 88.107 kb on - strand, within PP_0077at 88.107 kb on - strand, within PP_0077at 88.129 kb on - strand, within PP_0077at 88.140 kb on + strand, within PP_0077at 88.140 kb on + strand, within PP_0077at 88.141 kb on - strand, within PP_0077at 88.189 kb on + strand, within PP_0077at 88.190 kb on - strand, within PP_0077at 88.191 kb on + strand, within PP_0077at 88.191 kb on + strand, within PP_0077at 88.191 kb on + strand, within PP_0077at 88.191 kb on + strand, within PP_0077at 88.191 kb on + strand, within PP_0077at 88.191 kb on + strand, within PP_0077at 88.192 kb on - strand, within PP_0077at 88.192 kb on - strand, within PP_0077at 88.192 kb on - strand, within PP_0077at 88.192 kb on - strand, within PP_0077at 88.416 kb on + strand, within PP_0077at 88.416 kb on + strand, within PP_0077at 88.533 kb on + strand, within PP_0077at 88.535 kb on - strand, within PP_0077at 88.536 kb on + strand, within PP_0077at 88.536 kb on + strand, within PP_0077at 88.536 kb on + strand, within PP_0077at 88.537 kb on - strand, within PP_0077at 88.537 kb on - strand, within PP_0077at 88.537 kb on - strand, within PP_0077at 88.537 kb on - strand, within PP_0077at 88.537 kb on - strand, within PP_0077at 88.554 kb on + strand, within PP_0077at 88.579 kb on + strand, within PP_0077at 88.611 kb on + strand, within PP_0077at 88.611 kb on + strand, within PP_0077at 88.611 kb on + strand, within PP_0077at 88.611 kb on + strand, within PP_0077at 88.695 kb on + strand, within PP_0077at 88.725 kb on + strand, within PP_0077at 88.725 kb on + strand, within PP_0077at 88.726 kb on - strand, within PP_0077at 88.726 kb on - strand, within PP_0077at 88.726 kb on - strand, within PP_0077

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
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86,031 + PP_0075 0.56 +0.5
86,088 + PP_0075 0.60 +1.0
86,088 + PP_0075 0.60 -0.5
86,088 + PP_0075 0.60 -0.3
86,089 - PP_0075 0.60 +0.9
86,089 - PP_0075 0.60 -0.7
86,089 - PP_0075 0.60 -0.1
86,172 + PP_0075 0.65 +0.8
86,172 + PP_0075 0.65 -1.0
86,172 + PP_0075 0.65 -0.6
86,262 + PP_0075 0.71 +0.1
86,262 + PP_0075 0.71 -0.7
86,263 - PP_0075 0.71 -1.6
86,416 + PP_0075 0.81 +0.6
86,417 - PP_0075 0.81 -0.4
86,501 - PP_0075 0.86 +1.3
86,501 - PP_0075 0.86 -0.1
86,551 + PP_0075 0.89 +0.9
86,634 + -1.7
86,634 + -2.5
86,634 + +0.6
86,635 - -1.1
86,700 + +0.3
86,701 - -1.8
86,863 + -0.2
86,864 - +1.2
86,864 - +1.2
86,998 - PP_0076 0.15 -1.7
86,998 - PP_0076 0.15 -3.3
86,998 - PP_0076 0.15 +1.4
86,998 - PP_0076 0.15 +1.2
86,998 - PP_0076 0.15 -3.0
87,106 + PP_0076 0.26 -0.3
87,107 - PP_0076 0.27 +0.6
87,107 - PP_0076 0.27 +1.2
87,107 - PP_0076 0.27 -1.4
87,107 - PP_0076 0.27 -1.6
87,107 - PP_0076 0.27 -0.7
87,107 - PP_0076 0.27 -2.4
87,107 - PP_0076 0.27 -0.6
87,107 - PP_0076 0.27 -0.3
87,108 + PP_0076 0.27 -3.1
87,109 - PP_0076 0.27 -0.8
87,109 - PP_0076 0.27 -0.9
87,109 - PP_0076 0.27 -0.6
87,151 + PP_0076 0.31 -0.6
87,151 + PP_0076 0.31 +0.1
87,152 - PP_0076 0.31 -2.5
87,427 + PP_0076 0.61 +0.3
87,427 + PP_0076 0.61 -0.1
87,427 + PP_0076 0.61 -0.8
87,427 + PP_0076 0.61 +1.2
87,428 - PP_0076 0.61 -1.0
87,428 - PP_0076 0.61 -1.8
87,428 - PP_0076 0.61 -0.0
87,580 + PP_0076 0.78 +0.2
87,580 + PP_0076 0.78 -0.5
87,580 + PP_0076 0.78 -1.4
87,581 - PP_0076 0.78 -2.8
87,636 + PP_0076 0.84 +0.1
87,669 - -0.3
87,669 - PP_0076 0.87 +0.3
87,873 + -1.0
87,873 + +1.2
87,873 + -0.1
87,873 + -1.3
87,873 + +0.1
87,873 + -1.3
87,873 + +0.5
87,874 - +0.8
87,874 - -1.5
87,874 - -1.0
87,874 - -1.0
87,874 - -0.3
87,974 + PP_0077 0.11 +0.4
87,974 + PP_0077 0.11 -0.3
87,975 - PP_0077 0.12 -0.2
88,101 + PP_0077 0.20 +0.4
88,102 - PP_0077 0.20 -0.8
88,102 - PP_0077 0.20 -0.4
88,102 - PP_0077 0.20 +0.7
88,102 - PP_0077 0.20 -1.4
88,106 + PP_0077 0.20 +1.0
88,106 + PP_0077 0.20 +0.5
88,106 + PP_0077 0.20 +0.9
88,107 - PP_0077 0.20 -0.4
88,107 - PP_0077 0.20 -1.9
88,129 - PP_0077 0.22 +0.1
88,140 + PP_0077 0.22 -1.7
88,140 + PP_0077 0.22 -0.3
88,141 - PP_0077 0.22 +0.7
88,189 + PP_0077 0.26 -0.4
88,190 - PP_0077 0.26 +1.0
88,191 + PP_0077 0.26 +1.1
88,191 + PP_0077 0.26 -0.4
88,191 + PP_0077 0.26 -0.4
88,191 + PP_0077 0.26 -0.2
88,191 + PP_0077 0.26 +0.4
88,191 + PP_0077 0.26 -1.7
88,192 - PP_0077 0.26 +0.0
88,192 - PP_0077 0.26 +0.5
88,192 - PP_0077 0.26 +0.4
88,192 - PP_0077 0.26 -2.6
88,416 + PP_0077 0.41 -1.9
88,416 + PP_0077 0.41 -4.2
88,533 + PP_0077 0.48 +0.7
88,535 - PP_0077 0.48 +0.4
88,536 + PP_0077 0.48 -0.5
88,536 + PP_0077 0.48 +0.9
88,536 + PP_0077 0.48 -3.2
88,537 - PP_0077 0.49 +1.0
88,537 - PP_0077 0.49 -0.5
88,537 - PP_0077 0.49 +0.9
88,537 - PP_0077 0.49 +1.1
88,537 - PP_0077 0.49 -2.1
88,554 + PP_0077 0.50 +1.6
88,579 + PP_0077 0.51 -1.4
88,611 + PP_0077 0.53 -0.5
88,611 + PP_0077 0.53 +0.7
88,611 + PP_0077 0.53 +1.1
88,611 + PP_0077 0.53 -0.4
88,695 + PP_0077 0.59 +2.6
88,725 + PP_0077 0.61 -0.8
88,725 + PP_0077 0.61 +0.3
88,726 - PP_0077 0.61 -0.6
88,726 - PP_0077 0.61 +1.6
88,726 - PP_0077 0.61 -0.1

Or see this region's nucleotide sequence