Experiment: P. simiae OR1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23705 and PS417_23710 overlap by 4 nucleotides PS417_23710 and PS417_23715 are separated by 385 nucleotides PS417_23715 and PS417_23720 are separated by 48 nucleotides
PS417_23705: PS417_23705 - Tryptophan 2,3-dioxygenase (EC 1.13.11.11) <small>(from data)</small>, at 5,125,994 to 5,126,851
_23705
PS417_23710: PS417_23710 - Kynurenine formamidase, bacterial (EC 3.5.1.9) <small>(from data)</small>, at 5,126,848 to 5,127,498
_23710
PS417_23715: PS417_23715 - cupin, at 5,127,884 to 5,128,228
_23715
PS417_23720: PS417_23720 - 3-hydroxyisobutyrate dehydrogenase, at 5,128,277 to 5,129,164
_23720
Position (kb) 5126
5127
5128 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 5125.899 kb on - strand at 5125.995 kb on + strand at 5126.058 kb on + strand at 5126.058 kb on + strand at 5126.185 kb on - strand, within PS417_23705 at 5126.214 kb on + strand, within PS417_23705 at 5126.422 kb on + strand, within PS417_23705 at 5126.505 kb on + strand, within PS417_23705 at 5126.505 kb on + strand, within PS417_23705 at 5126.757 kb on + strand, within PS417_23705 at 5127.158 kb on + strand, within PS417_23710 at 5127.189 kb on + strand, within PS417_23710 at 5127.189 kb on + strand, within PS417_23710 at 5127.269 kb on + strand, within PS417_23710 at 5127.269 kb on + strand, within PS417_23710 at 5127.269 kb on + strand, within PS417_23710 at 5127.270 kb on - strand, within PS417_23710 at 5127.314 kb on + strand, within PS417_23710 at 5127.315 kb on - strand, within PS417_23710 at 5127.532 kb on + strand at 5127.533 kb on - strand at 5127.594 kb on + strand at 5127.622 kb on + strand at 5127.622 kb on + strand at 5127.626 kb on + strand at 5127.627 kb on - strand at 5127.627 kb on - strand at 5127.627 kb on - strand at 5127.627 kb on - strand at 5127.629 kb on - strand at 5127.629 kb on - strand at 5127.696 kb on + strand at 5127.710 kb on + strand at 5127.710 kb on + strand at 5127.710 kb on + strand at 5127.710 kb on + strand at 5127.711 kb on - strand at 5127.711 kb on - strand at 5127.711 kb on - strand at 5127.711 kb on - strand at 5127.711 kb on - strand at 5127.903 kb on + strand at 5128.052 kb on + strand, within PS417_23715 at 5128.052 kb on + strand, within PS417_23715 at 5128.052 kb on + strand, within PS417_23715 at 5128.052 kb on + strand at 5128.053 kb on - strand, within PS417_23715 at 5128.061 kb on + strand, within PS417_23715 at 5128.062 kb on - strand, within PS417_23715 at 5128.062 kb on - strand, within PS417_23715 at 5128.098 kb on - strand, within PS417_23715 at 5128.207 kb on + strand at 5128.208 kb on - strand at 5128.279 kb on - strand at 5128.300 kb on + strand at 5128.301 kb on - strand at 5128.304 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae OR1 MOI 0.1 remove 5,125,899 - -1.6 5,125,995 + +2.6 5,126,058 + -0.5 5,126,058 + +0.0 5,126,185 - PS417_23705 0.22 -0.3 5,126,214 + PS417_23705 0.26 +1.8 5,126,422 + PS417_23705 0.50 +1.3 5,126,505 + PS417_23705 0.60 +0.2 5,126,505 + PS417_23705 0.60 -1.2 5,126,757 + PS417_23705 0.89 +1.6 5,127,158 + PS417_23710 0.48 -1.6 5,127,189 + PS417_23710 0.52 -1.4 5,127,189 + PS417_23710 0.52 -0.7 5,127,269 + PS417_23710 0.65 -0.0 5,127,269 + PS417_23710 0.65 -1.6 5,127,269 + PS417_23710 0.65 +0.5 5,127,270 - PS417_23710 0.65 +0.3 5,127,314 + PS417_23710 0.72 -3.4 5,127,315 - PS417_23710 0.72 -1.5 5,127,532 + +0.2 5,127,533 - -0.2 5,127,594 + -1.7 5,127,622 + +0.3 5,127,622 + +0.4 5,127,626 + -2.4 5,127,627 - +3.2 5,127,627 - -1.2 5,127,627 - -1.4 5,127,627 - +0.9 5,127,629 - +0.5 5,127,629 - +2.0 5,127,696 + -0.2 5,127,710 + -1.6 5,127,710 + -0.8 5,127,710 + +0.3 5,127,710 + +1.8 5,127,711 - -1.0 5,127,711 - +0.8 5,127,711 - +1.9 5,127,711 - -3.6 5,127,711 - -0.5 5,127,903 + -1.8 5,128,052 + PS417_23715 0.49 -0.6 5,128,052 + PS417_23715 0.49 +2.2 5,128,052 + PS417_23715 0.49 -1.7 5,128,052 + -0.4 5,128,053 - PS417_23715 0.49 -0.1 5,128,061 + PS417_23715 0.51 -1.6 5,128,062 - PS417_23715 0.52 -1.2 5,128,062 - PS417_23715 0.52 -1.8 5,128,098 - PS417_23715 0.62 -0.4 5,128,207 + +1.2 5,128,208 - -3.9 5,128,279 - +0.7 5,128,300 + +0.7 5,128,301 - +0.1 5,128,304 - -1.3
Or see this region's nucleotide sequence