Strain Fitness in Pseudomonas simiae WCS417 around PS417_14520

Experiment: P. simiae OR1 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_14510 and PS417_14515 overlap by 4 nucleotidesPS417_14515 and PS417_14520 overlap by 4 nucleotidesPS417_14520 and PS417_14525 overlap by 4 nucleotidesPS417_14525 and PS417_14530 are separated by 178 nucleotides PS417_14510: PS417_14510 - acyl-CoA dehydrogenase, at 3,173,559 to 3,174,557 _14510 PS417_14515: PS417_14515 - acetylglucosaminylphosphatidylinositol deacetylase, at 3,174,554 to 3,175,312 _14515 PS417_14520: PS417_14520 - methyltransferase, at 3,175,309 to 3,175,908 _14520 PS417_14525: PS417_14525 - glycosyl transferase, at 3,175,905 to 3,176,564 _14525 PS417_14530: PS417_14530 - acetylglucosaminylphosphatidylinositol deacetylase, at 3,176,743 to 3,177,504 _14530 Position (kb) 3175 3176Strain fitness (log2 ratio) -2 -1 0 1 2at 3174.471 kb on + strandat 3174.471 kb on + strandat 3174.471 kb on + strandat 3174.472 kb on - strandat 3174.472 kb on - strandat 3174.969 kb on - strand, within PS417_14515at 3175.309 kb on - strandat 3175.436 kb on - strand, within PS417_14520at 3175.638 kb on - strand, within PS417_14520at 3175.655 kb on - strand, within PS417_14520at 3175.655 kb on - strand, within PS417_14520at 3176.376 kb on + strand, within PS417_14525at 3176.577 kb on + strandat 3176.578 kb on - strandat 3176.626 kb on + strandat 3176.827 kb on - strand, within PS417_14530

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Per-strain Table

Position Strand Gene LocusTag Fraction P. simiae OR1 MOI 0.1
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3,174,471 + -0.8
3,174,471 + +0.4
3,174,471 + -0.5
3,174,472 - -0.4
3,174,472 - +0.9
3,174,969 - PS417_14515 0.55 +1.0
3,175,309 - -0.5
3,175,436 - PS417_14520 0.21 +0.8
3,175,638 - PS417_14520 0.55 -1.0
3,175,655 - PS417_14520 0.58 +1.8
3,175,655 - PS417_14520 0.58 +0.8
3,176,376 + PS417_14525 0.71 -2.1
3,176,577 + +0.3
3,176,578 - -0.1
3,176,626 + -0.4
3,176,827 - PS417_14530 0.11 -0.9

Or see this region's nucleotide sequence