Experiment: P. simiae OR1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14510 and PS417_14515 overlap by 4 nucleotides PS417_14515 and PS417_14520 overlap by 4 nucleotides PS417_14520 and PS417_14525 overlap by 4 nucleotides PS417_14525 and PS417_14530 are separated by 178 nucleotides
PS417_14510: PS417_14510 - acyl-CoA dehydrogenase, at 3,173,559 to 3,174,557
_14510
PS417_14515: PS417_14515 - acetylglucosaminylphosphatidylinositol deacetylase, at 3,174,554 to 3,175,312
_14515
PS417_14520: PS417_14520 - methyltransferase, at 3,175,309 to 3,175,908
_14520
PS417_14525: PS417_14525 - glycosyl transferase, at 3,175,905 to 3,176,564
_14525
PS417_14530: PS417_14530 - acetylglucosaminylphosphatidylinositol deacetylase, at 3,176,743 to 3,177,504
_14530
Position (kb)
3175
3176 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3174.471 kb on + strand at 3174.471 kb on + strand at 3174.471 kb on + strand at 3174.472 kb on - strand at 3174.472 kb on - strand at 3174.969 kb on - strand, within PS417_14515 at 3175.309 kb on - strand at 3175.436 kb on - strand, within PS417_14520 at 3175.638 kb on - strand, within PS417_14520 at 3175.655 kb on - strand, within PS417_14520 at 3175.655 kb on - strand, within PS417_14520 at 3176.376 kb on + strand, within PS417_14525 at 3176.577 kb on + strand at 3176.578 kb on - strand at 3176.626 kb on + strand at 3176.827 kb on - strand, within PS417_14530
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae OR1 MOI 0.1 remove 3,174,471 + -0.8 3,174,471 + +0.4 3,174,471 + -0.5 3,174,472 - -0.4 3,174,472 - +0.9 3,174,969 - PS417_14515 0.55 +1.0 3,175,309 - -0.5 3,175,436 - PS417_14520 0.21 +0.8 3,175,638 - PS417_14520 0.55 -1.0 3,175,655 - PS417_14520 0.58 +1.8 3,175,655 - PS417_14520 0.58 +0.8 3,176,376 + PS417_14525 0.71 -2.1 3,176,577 + +0.3 3,176,578 - -0.1 3,176,626 + -0.4 3,176,827 - PS417_14530 0.11 -0.9
Or see this region's nucleotide sequence