Experiment: P. simiae OR1 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_08955 and PS417_08960 are separated by 64 nucleotides PS417_08960 and PS417_08965 are separated by 140 nucleotides PS417_08965 and PS417_08970 are separated by 95 nucleotides
PS417_08955: PS417_08955 - pimeloyl-CoA dehydrogenase, at 1,949,531 to 1,950,397
_08955
PS417_08960: PS417_08960 - peroxiredoxin, at 1,950,462 to 1,950,878
_08960
PS417_08965: PS417_08965 - 5-oxo-L-prolinase, at 1,951,019 to 1,952,041
_08965
PS417_08970: PS417_08970 - chemotaxis protein, at 1,952,137 to 1,953,762
_08970
Position (kb)
1951
1952
1953 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1950.088 kb on + strand, within PS417_08955 at 1950.089 kb on - strand, within PS417_08955 at 1950.089 kb on - strand, within PS417_08955 at 1950.201 kb on + strand, within PS417_08955 at 1950.201 kb on + strand at 1950.202 kb on - strand, within PS417_08955 at 1950.202 kb on - strand, within PS417_08955 at 1950.202 kb on - strand, within PS417_08955 at 1950.224 kb on - strand, within PS417_08955 at 1950.281 kb on - strand, within PS417_08955 at 1950.281 kb on - strand, within PS417_08955 at 1950.281 kb on - strand, within PS417_08955 at 1950.437 kb on + strand at 1950.619 kb on + strand, within PS417_08960 at 1950.619 kb on + strand, within PS417_08960 at 1950.619 kb on + strand, within PS417_08960 at 1950.619 kb on + strand at 1950.620 kb on - strand, within PS417_08960 at 1950.661 kb on - strand, within PS417_08960 at 1950.661 kb on - strand, within PS417_08960 at 1950.661 kb on - strand, within PS417_08960 at 1950.661 kb on - strand, within PS417_08960 at 1950.700 kb on + strand, within PS417_08960 at 1950.749 kb on - strand, within PS417_08960 at 1950.749 kb on - strand, within PS417_08960 at 1950.749 kb on - strand, within PS417_08960 at 1950.824 kb on + strand, within PS417_08960 at 1950.881 kb on + strand at 1950.882 kb on - strand at 1950.918 kb on + strand at 1950.918 kb on + strand at 1950.919 kb on - strand at 1950.919 kb on - strand at 1950.920 kb on + strand at 1950.920 kb on + strand at 1950.920 kb on + strand at 1951.016 kb on - strand at 1951.094 kb on + strand at 1951.254 kb on - strand, within PS417_08965 at 1951.280 kb on + strand, within PS417_08965 at 1951.280 kb on + strand, within PS417_08965 at 1951.281 kb on - strand, within PS417_08965 at 1951.286 kb on + strand, within PS417_08965 at 1951.538 kb on + strand, within PS417_08965 at 1951.539 kb on - strand, within PS417_08965 at 1951.539 kb on - strand, within PS417_08965 at 1951.539 kb on - strand, within PS417_08965 at 1951.616 kb on + strand, within PS417_08965 at 1951.617 kb on - strand, within PS417_08965 at 1951.772 kb on + strand, within PS417_08965 at 1951.773 kb on - strand, within PS417_08965 at 1951.877 kb on - strand, within PS417_08965 at 1952.058 kb on + strand at 1952.142 kb on + strand at 1952.143 kb on - strand at 1952.307 kb on + strand, within PS417_08970 at 1952.307 kb on + strand, within PS417_08970 at 1952.440 kb on + strand, within PS417_08970 at 1952.440 kb on + strand, within PS417_08970 at 1952.456 kb on + strand at 1952.457 kb on - strand, within PS417_08970 at 1952.477 kb on + strand, within PS417_08970 at 1952.478 kb on - strand, within PS417_08970 at 1952.509 kb on + strand, within PS417_08970 at 1952.509 kb on + strand, within PS417_08970 at 1952.688 kb on - strand, within PS417_08970 at 1952.775 kb on + strand, within PS417_08970
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae OR1 MOI 0.1 remove 1,950,088 + PS417_08955 0.64 +0.2 1,950,089 - PS417_08955 0.64 -0.3 1,950,089 - PS417_08955 0.64 -1.0 1,950,201 + PS417_08955 0.77 +0.3 1,950,201 + -0.3 1,950,202 - PS417_08955 0.77 -0.0 1,950,202 - PS417_08955 0.77 -0.3 1,950,202 - PS417_08955 0.77 +0.3 1,950,224 - PS417_08955 0.80 +0.2 1,950,281 - PS417_08955 0.87 +0.8 1,950,281 - PS417_08955 0.87 -2.4 1,950,281 - PS417_08955 0.87 +0.8 1,950,437 + -3.0 1,950,619 + PS417_08960 0.38 -0.5 1,950,619 + PS417_08960 0.38 +1.0 1,950,619 + PS417_08960 0.38 +0.0 1,950,619 + -0.7 1,950,620 - PS417_08960 0.38 -1.0 1,950,661 - PS417_08960 0.48 -1.3 1,950,661 - PS417_08960 0.48 -1.3 1,950,661 - PS417_08960 0.48 -1.3 1,950,661 - PS417_08960 0.48 +2.4 1,950,700 + PS417_08960 0.57 -0.4 1,950,749 - PS417_08960 0.69 +0.3 1,950,749 - PS417_08960 0.69 -2.3 1,950,749 - PS417_08960 0.69 -2.2 1,950,824 + PS417_08960 0.87 -2.2 1,950,881 + +1.3 1,950,882 - -3.2 1,950,918 + -1.0 1,950,918 + +1.3 1,950,919 - +0.2 1,950,919 - +1.0 1,950,920 + -2.1 1,950,920 + +0.3 1,950,920 + +0.3 1,951,016 - -0.8 1,951,094 + +0.2 1,951,254 - PS417_08965 0.23 +0.1 1,951,280 + PS417_08965 0.26 -2.2 1,951,280 + PS417_08965 0.26 +0.0 1,951,281 - PS417_08965 0.26 -0.0 1,951,286 + PS417_08965 0.26 -0.5 1,951,538 + PS417_08965 0.51 +0.3 1,951,539 - PS417_08965 0.51 -2.0 1,951,539 - PS417_08965 0.51 +0.1 1,951,539 - PS417_08965 0.51 -0.6 1,951,616 + PS417_08965 0.58 -3.1 1,951,617 - PS417_08965 0.58 -0.4 1,951,772 + PS417_08965 0.74 -1.4 1,951,773 - PS417_08965 0.74 -2.0 1,951,877 - PS417_08965 0.84 -0.1 1,952,058 + -0.8 1,952,142 + -0.1 1,952,143 - -0.4 1,952,307 + PS417_08970 0.10 -0.1 1,952,307 + PS417_08970 0.10 +0.6 1,952,440 + PS417_08970 0.19 -1.2 1,952,440 + PS417_08970 0.19 -2.5 1,952,456 + -1.5 1,952,457 - PS417_08970 0.20 -0.7 1,952,477 + PS417_08970 0.21 -1.9 1,952,478 - PS417_08970 0.21 -1.3 1,952,509 + PS417_08970 0.23 +0.4 1,952,509 + PS417_08970 0.23 +0.1 1,952,688 - PS417_08970 0.34 -2.2 1,952,775 + PS417_08970 0.39 +0.6
Or see this region's nucleotide sequence