Experiment: Streptomycin 200 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1259 and Atu1260 are separated by 84 nucleotides Atu1260 and Atu1261 are separated by 83 nucleotides Atu1261 and Atu1262 are separated by 289 nucleotides Atu1262 and Atu1263 are separated by 343 nucleotides
Atu1259: Atu1259 - ATP-dependent Clp protease, ATP-binding subunit, at 1,247,190 to 1,248,467
Atu1259
Atu1260: Atu1260 - hypothetical protein, at 1,248,552 to 1,248,797
Atu1260
Atu1261: Atu1261 - ATP-dependent protease LA, at 1,248,881 to 1,251,298
Atu1261
Atu1262: Atu1262 - histone-like protein, at 1,251,588 to 1,251,863
Atu1262
Atu1263: Atu1263 - hypothetical protein, at 1,252,207 to 1,254,405
Atu1263
Position (kb) 1248
1249
1250
1251
1252 Strain fitness (log2 ratio) -2
-1
0
1 at 1248.419 kb on + strand at 1248.562 kb on - strand at 1248.594 kb on - strand, within Atu1260 at 1248.831 kb on + strand at 1251.428 kb on + strand at 1251.773 kb on + strand, within Atu1262 at 1252.033 kb on + strand at 1252.236 kb on + strand at 1252.248 kb on + strand at 1252.248 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Streptomycin 200 ug/mL remove 1,248,419 + -2.7 1,248,562 - -0.7 1,248,594 - Atu1260 0.17 +0.0 1,248,831 + -0.5 1,251,428 + -0.6 1,251,773 + Atu1262 0.67 +0.4 1,252,033 + -1.0 1,252,236 + +0.4 1,252,248 + -1.0 1,252,248 + +0.2
Or see this region's nucleotide sequence