Experiment: Streptomycin 200 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1258 and Atu1259 are separated by 291 nucleotides Atu1259 and Atu1260 are separated by 84 nucleotides Atu1260 and Atu1261 are separated by 83 nucleotides
Atu1258: Atu1258 - ATP-dependent Clp protease, proteolytic subunit, at 1,246,266 to 1,246,898
Atu1258
Atu1259: Atu1259 - ATP-dependent Clp protease, ATP-binding subunit, at 1,247,190 to 1,248,467
Atu1259
Atu1260: Atu1260 - hypothetical protein, at 1,248,552 to 1,248,797
Atu1260
Atu1261: Atu1261 - ATP-dependent protease LA, at 1,248,881 to 1,251,298
Atu1261
Position (kb)
1247
1248
1249 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1246.190 kb on - strand at 1246.208 kb on + strand at 1246.208 kb on + strand at 1246.242 kb on + strand at 1246.242 kb on + strand at 1246.250 kb on - strand at 1246.586 kb on - strand, within Atu1258 at 1246.586 kb on - strand, within Atu1258 at 1246.616 kb on + strand, within Atu1258 at 1246.646 kb on + strand, within Atu1258 at 1246.731 kb on - strand, within Atu1258 at 1246.832 kb on + strand, within Atu1258 at 1246.840 kb on - strand at 1248.419 kb on + strand at 1248.562 kb on - strand at 1248.594 kb on - strand, within Atu1260 at 1248.831 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Streptomycin 200 ug/mL remove 1,246,190 - -0.5 1,246,208 + -1.3 1,246,208 + +0.0 1,246,242 + -2.0 1,246,242 + -1.6 1,246,250 - -0.9 1,246,586 - Atu1258 0.51 -2.0 1,246,586 - Atu1258 0.51 -0.6 1,246,616 + Atu1258 0.55 -1.5 1,246,646 + Atu1258 0.60 -3.2 1,246,731 - Atu1258 0.73 -1.3 1,246,832 + Atu1258 0.89 -0.5 1,246,840 - -0.2 1,248,419 + -2.7 1,248,562 - -0.7 1,248,594 - Atu1260 0.17 +0.0 1,248,831 + -0.5
Or see this region's nucleotide sequence