Experiment: Streptomycin 200 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4421 and Atu4422 are separated by 166 nucleotides Atu4422 and Atu4423 overlap by 4 nucleotides Atu4423 and Atu4424 overlap by 8 nucleotides
Atu4421: Atu4421 - spermidine/putrescine ABC transporter substrate-binding protein, at 1,562,940 to 1,563,962
Atu4421
Atu4422: Atu4422 - spermidine/putrescine ABC transporter permease, at 1,564,129 to 1,564,956
Atu4422
Atu4423: Atu4423 - ABC transporter permease, at 1,564,953 to 1,565,732
Atu4423
Atu4424: Atu4424 - spermidine/putrescine ABC transporter ATPase, at 1,565,725 to 1,566,750
Atu4424
Position (kb)
1564
1565 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1563.266 kb on + strand, within Atu4421 at 1563.484 kb on + strand, within Atu4421 at 1563.598 kb on - strand, within Atu4421 at 1563.653 kb on + strand, within Atu4421 at 1563.664 kb on + strand, within Atu4421 at 1563.678 kb on - strand, within Atu4421 at 1563.731 kb on - strand, within Atu4421 at 1563.738 kb on - strand, within Atu4421 at 1564.042 kb on - strand at 1564.196 kb on + strand at 1564.238 kb on - strand, within Atu4422 at 1564.253 kb on - strand, within Atu4422 at 1564.321 kb on - strand, within Atu4422 at 1564.364 kb on - strand, within Atu4422 at 1564.453 kb on + strand, within Atu4422 at 1564.460 kb on + strand, within Atu4422 at 1564.517 kb on + strand, within Atu4422 at 1564.530 kb on - strand, within Atu4422 at 1564.530 kb on - strand, within Atu4422 at 1564.530 kb on - strand, within Atu4422 at 1564.706 kb on + strand, within Atu4422 at 1564.732 kb on + strand, within Atu4422 at 1564.757 kb on - strand, within Atu4422 at 1564.771 kb on + strand, within Atu4422 at 1564.775 kb on + strand, within Atu4422 at 1564.779 kb on - strand, within Atu4422 at 1564.880 kb on + strand at 1564.880 kb on + strand at 1564.944 kb on + strand at 1564.952 kb on - strand at 1565.023 kb on - strand at 1565.102 kb on + strand, within Atu4423 at 1565.207 kb on - strand, within Atu4423 at 1565.290 kb on + strand, within Atu4423 at 1565.300 kb on + strand, within Atu4423 at 1565.348 kb on + strand, within Atu4423 at 1565.348 kb on + strand, within Atu4423 at 1565.433 kb on - strand, within Atu4423 at 1565.575 kb on - strand, within Atu4423 at 1565.578 kb on + strand, within Atu4423 at 1565.612 kb on + strand, within Atu4423 at 1565.615 kb on - strand, within Atu4423 at 1565.835 kb on + strand, within Atu4424 at 1565.843 kb on - strand, within Atu4424 at 1565.874 kb on + strand, within Atu4424 at 1565.884 kb on - strand, within Atu4424 at 1565.932 kb on - strand, within Atu4424 at 1565.947 kb on + strand, within Atu4424
Per-strain Table
Position Strand Gene LocusTag Fraction Streptomycin 200 ug/mL remove 1,563,266 + Atu4421 0.32 -0.3 1,563,484 + Atu4421 0.53 -0.9 1,563,598 - Atu4421 0.64 -0.3 1,563,653 + Atu4421 0.70 -0.6 1,563,664 + Atu4421 0.71 -0.2 1,563,678 - Atu4421 0.72 -0.5 1,563,731 - Atu4421 0.77 -0.8 1,563,738 - Atu4421 0.78 -2.3 1,564,042 - +2.6 1,564,196 + -0.2 1,564,238 - Atu4422 0.13 -0.2 1,564,253 - Atu4422 0.15 -1.0 1,564,321 - Atu4422 0.23 -0.8 1,564,364 - Atu4422 0.28 -0.0 1,564,453 + Atu4422 0.39 -0.1 1,564,460 + Atu4422 0.40 +0.1 1,564,517 + Atu4422 0.47 +0.3 1,564,530 - Atu4422 0.48 -0.4 1,564,530 - Atu4422 0.48 -1.0 1,564,530 - Atu4422 0.48 +0.6 1,564,706 + Atu4422 0.70 -0.7 1,564,732 + Atu4422 0.73 -0.1 1,564,757 - Atu4422 0.76 -0.9 1,564,771 + Atu4422 0.78 -0.1 1,564,775 + Atu4422 0.78 -1.4 1,564,779 - Atu4422 0.79 -0.6 1,564,880 + +0.7 1,564,880 + -0.8 1,564,944 + +1.0 1,564,952 - -0.8 1,565,023 - -0.2 1,565,102 + Atu4423 0.19 -0.2 1,565,207 - Atu4423 0.33 -0.0 1,565,290 + Atu4423 0.43 -0.1 1,565,300 + Atu4423 0.44 +2.0 1,565,348 + Atu4423 0.51 -0.2 1,565,348 + Atu4423 0.51 -0.2 1,565,433 - Atu4423 0.62 +0.5 1,565,575 - Atu4423 0.80 -0.1 1,565,578 + Atu4423 0.80 -0.3 1,565,612 + Atu4423 0.84 -1.0 1,565,615 - Atu4423 0.85 -1.4 1,565,835 + Atu4424 0.11 -0.1 1,565,843 - Atu4424 0.12 -0.8 1,565,874 + Atu4424 0.15 -0.4 1,565,884 - Atu4424 0.15 -0.9 1,565,932 - Atu4424 0.20 +0.9 1,565,947 + Atu4424 0.22 +0.2
Or see this region's nucleotide sequence