Experiment: Streptomycin 200 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2552 and Atu2553 are separated by 120 nucleotides Atu2553 and Atu2554 are separated by 251 nucleotides Atu2554 and Atu2555 overlap by 31 nucleotides
Atu2552: Atu2552 - nodulation protein T precursor, at 2,525,519 to 2,526,973
Atu2552
Atu2553: Atu2553 - elongation factor P, at 2,527,094 to 2,527,663
Atu2553
Atu2554: Atu2554 - lysyl-tRNA synthetase, at 2,527,915 to 2,528,979
Atu2554
Atu2555: Atu2555 - L-lysine 2,3-aminomutase, at 2,528,949 to 2,530,040
Atu2555
Position (kb)
2527
2528
2529 Strain fitness (log2 ratio)
-2
-1
0
1 at 2526.975 kb on - strand at 2526.975 kb on - strand at 2527.069 kb on - strand at 2527.086 kb on - strand at 2527.716 kb on - strand at 2527.802 kb on + strand at 2527.934 kb on - strand at 2527.952 kb on + strand at 2527.962 kb on - strand at 2528.125 kb on - strand, within Atu2554 at 2528.152 kb on + strand, within Atu2554 at 2528.214 kb on - strand, within Atu2554 at 2528.513 kb on + strand, within Atu2554 at 2528.574 kb on - strand, within Atu2554 at 2529.245 kb on + strand, within Atu2555 at 2529.245 kb on + strand, within Atu2555 at 2529.335 kb on + strand, within Atu2555 at 2529.494 kb on + strand, within Atu2555 at 2529.568 kb on - strand, within Atu2555 at 2529.660 kb on - strand, within Atu2555 at 2529.771 kb on + strand, within Atu2555 at 2529.948 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Streptomycin 200 ug/mL remove 2,526,975 - -0.3 2,526,975 - +0.1 2,527,069 - +0.3 2,527,086 - +1.6 2,527,716 - -1.8 2,527,802 + +0.8 2,527,934 - +0.1 2,527,952 + -0.0 2,527,962 - -1.6 2,528,125 - Atu2554 0.20 +0.6 2,528,152 + Atu2554 0.22 +1.0 2,528,214 - Atu2554 0.28 -1.8 2,528,513 + Atu2554 0.56 +0.6 2,528,574 - Atu2554 0.62 -2.5 2,529,245 + Atu2555 0.27 -1.7 2,529,245 + Atu2555 0.27 +0.2 2,529,335 + Atu2555 0.35 -0.4 2,529,494 + Atu2555 0.50 -0.8 2,529,568 - Atu2555 0.57 -0.0 2,529,660 - Atu2555 0.65 +1.6 2,529,771 + Atu2555 0.75 -1.0 2,529,948 - -0.8
Or see this region's nucleotide sequence