Experiment: Streptomycin 200 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0007 and Atu0008 are separated by 185 nucleotides Atu0008 and Atu0009 overlap by 4 nucleotides Atu0009 and Atu0010 overlap by 4 nucleotides Atu0010 and Atu0011 overlap by 4 nucleotides
Atu0007: Atu0007 - hypothetical protein, at 4,493 to 5,029
Atu0007
Atu0008: Atu0008 - hypothetical protein, at 5,215 to 5,868
Atu0008
Atu0009: Atu0009 - membrane-bound lytic murein transglycosylase, at 5,865 to 6,977
Atu0009
Atu0010: Atu0010 - hypothetical protein, at 6,974 to 7,543
Atu0010
Atu0011: Atu0011 - transcriptional regulator, at 7,540 to 7,905
Atu0011
Position (kb) 5
6
7 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4.888 kb on + strand, within Atu0007 at 4.906 kb on - strand, within Atu0007 at 4.916 kb on - strand, within Atu0007 at 4.936 kb on + strand, within Atu0007 at 4.936 kb on + strand, within Atu0007 at 4.944 kb on - strand, within Atu0007 at 5.029 kb on + strand at 5.067 kb on + strand at 5.067 kb on + strand at 5.114 kb on + strand at 5.122 kb on - strand at 5.176 kb on - strand at 5.228 kb on - strand at 5.334 kb on + strand, within Atu0008 at 5.335 kb on + strand, within Atu0008 at 5.343 kb on - strand, within Atu0008 at 5.431 kb on - strand, within Atu0008 at 5.431 kb on - strand, within Atu0008 at 5.535 kb on + strand, within Atu0008 at 5.543 kb on - strand, within Atu0008 at 5.543 kb on - strand, within Atu0008 at 5.784 kb on + strand, within Atu0008 at 5.801 kb on - strand, within Atu0008 at 5.830 kb on - strand at 5.842 kb on - strand at 5.875 kb on - strand at 5.909 kb on + strand at 5.990 kb on - strand, within Atu0009 at 6.003 kb on + strand, within Atu0009 at 6.055 kb on - strand, within Atu0009 at 6.098 kb on + strand, within Atu0009 at 6.165 kb on - strand, within Atu0009 at 6.172 kb on + strand, within Atu0009 at 6.224 kb on - strand, within Atu0009 at 6.289 kb on - strand, within Atu0009 at 6.289 kb on - strand, within Atu0009 at 6.435 kb on + strand, within Atu0009 at 6.435 kb on + strand, within Atu0009 at 6.527 kb on - strand, within Atu0009 at 6.538 kb on - strand, within Atu0009 at 6.660 kb on + strand, within Atu0009 at 6.710 kb on - strand, within Atu0009 at 6.742 kb on + strand, within Atu0009 at 6.801 kb on - strand, within Atu0009 at 6.827 kb on + strand, within Atu0009 at 6.920 kb on - strand at 7.026 kb on - strand at 7.098 kb on + strand, within Atu0010 at 7.148 kb on + strand, within Atu0010 at 7.200 kb on + strand, within Atu0010 at 7.200 kb on - strand, within Atu0010 at 7.208 kb on - strand, within Atu0010 at 7.228 kb on + strand, within Atu0010 at 7.271 kb on + strand, within Atu0010 at 7.334 kb on + strand, within Atu0010 at 7.363 kb on - strand, within Atu0010 at 7.378 kb on - strand, within Atu0010 at 7.378 kb on - strand, within Atu0010 at 7.378 kb on - strand, within Atu0010 at 7.378 kb on - strand, within Atu0010 at 7.405 kb on + strand, within Atu0010 at 7.410 kb on + strand, within Atu0010 at 7.423 kb on - strand, within Atu0010 at 7.761 kb on + strand, within Atu0011 at 7.800 kb on + strand, within Atu0011 at 7.812 kb on + strand, within Atu0011 at 7.866 kb on + strand, within Atu0011 at 7.910 kb on + strand at 7.922 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Streptomycin 200 ug/mL remove 4,888 + Atu0007 0.74 -1.0 4,906 - Atu0007 0.77 -1.5 4,916 - Atu0007 0.79 -0.4 4,936 + Atu0007 0.82 -0.4 4,936 + Atu0007 0.82 -2.3 4,944 - Atu0007 0.84 -1.9 5,029 + +1.2 5,067 + -0.8 5,067 + -1.9 5,114 + -1.0 5,122 - -1.2 5,176 - -1.5 5,228 - -1.0 5,334 + Atu0008 0.18 -2.4 5,335 + Atu0008 0.18 +0.1 5,343 - Atu0008 0.20 -0.1 5,431 - Atu0008 0.33 -0.4 5,431 - Atu0008 0.33 -1.6 5,535 + Atu0008 0.49 -1.9 5,543 - Atu0008 0.50 +0.2 5,543 - Atu0008 0.50 -0.2 5,784 + Atu0008 0.87 -2.0 5,801 - Atu0008 0.90 -1.4 5,830 - -0.4 5,842 - +0.6 5,875 - -0.3 5,909 + -1.2 5,990 - Atu0009 0.11 -2.5 6,003 + Atu0009 0.12 -0.0 6,055 - Atu0009 0.17 -0.2 6,098 + Atu0009 0.21 -0.3 6,165 - Atu0009 0.27 -1.6 6,172 + Atu0009 0.28 -1.3 6,224 - Atu0009 0.32 -1.4 6,289 - Atu0009 0.38 -0.9 6,289 - Atu0009 0.38 -0.6 6,435 + Atu0009 0.51 -0.6 6,435 + Atu0009 0.51 -0.9 6,527 - Atu0009 0.59 -0.2 6,538 - Atu0009 0.60 -0.2 6,660 + Atu0009 0.71 +1.6 6,710 - Atu0009 0.76 -2.0 6,742 + Atu0009 0.79 -1.8 6,801 - Atu0009 0.84 -2.4 6,827 + Atu0009 0.86 -1.3 6,920 - -0.6 7,026 - +0.6 7,098 + Atu0010 0.22 -0.5 7,148 + Atu0010 0.31 -2.4 7,200 + Atu0010 0.40 +1.6 7,200 - Atu0010 0.40 +0.6 7,208 - Atu0010 0.41 -1.9 7,228 + Atu0010 0.45 -2.0 7,271 + Atu0010 0.52 +1.0 7,334 + Atu0010 0.63 +0.7 7,363 - Atu0010 0.68 +0.0 7,378 - Atu0010 0.71 -1.1 7,378 - Atu0010 0.71 -0.0 7,378 - Atu0010 0.71 -3.2 7,378 - Atu0010 0.71 -0.6 7,405 + Atu0010 0.76 -0.0 7,410 + Atu0010 0.76 -0.3 7,423 - Atu0010 0.79 -0.9 7,761 + Atu0011 0.60 -1.9 7,800 + Atu0011 0.71 -0.0 7,812 + Atu0011 0.74 -0.1 7,866 + Atu0011 0.89 -0.2 7,910 + -1.2 7,922 + -0.8
Or see this region's nucleotide sequence