Experiment: Chloramphenicol 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5498 and Atu5499 overlap by 4 nucleotides Atu5499 and Atu5500 overlap by 4 nucleotides Atu5500 and Atu5501 are separated by 343 nucleotides
Atu5498: Atu5498 - oxidoreductase with iron-sulfur subunit, at 488,249 to 488,782
Atu5498
Atu5499: Atu5499 - oxidoreductase, at 488,779 to 489,768
Atu5499
Atu5500: Atu5500 - dehydrogenase, at 489,765 to 492,068
Atu5500
Atu5501: Atu5501 - hypothetical protein, at 492,412 to 493,074
Atu5501
Position (kb)
489
490
491
492
493 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 488.777 kb on - strand at 488.862 kb on + strand at 488.900 kb on + strand, within Atu5499 at 488.908 kb on - strand, within Atu5499 at 488.935 kb on + strand, within Atu5499 at 488.943 kb on - strand, within Atu5499 at 488.943 kb on - strand, within Atu5499 at 488.977 kb on - strand, within Atu5499 at 488.984 kb on + strand, within Atu5499 at 488.989 kb on + strand, within Atu5499 at 488.997 kb on - strand, within Atu5499 at 489.002 kb on - strand, within Atu5499 at 489.002 kb on - strand, within Atu5499 at 489.030 kb on + strand, within Atu5499 at 489.056 kb on - strand, within Atu5499 at 489.060 kb on + strand, within Atu5499 at 489.243 kb on - strand, within Atu5499 at 489.260 kb on + strand, within Atu5499 at 489.291 kb on + strand, within Atu5499 at 489.481 kb on + strand, within Atu5499 at 489.481 kb on + strand, within Atu5499 at 489.489 kb on - strand, within Atu5499 at 489.525 kb on + strand, within Atu5499 at 489.527 kb on - strand, within Atu5499 at 489.540 kb on + strand, within Atu5499 at 489.736 kb on + strand at 489.833 kb on - strand at 489.833 kb on - strand at 489.869 kb on + strand at 489.870 kb on + strand at 489.871 kb on + strand at 489.878 kb on - strand at 489.878 kb on - strand at 489.878 kb on - strand at 489.879 kb on - strand at 489.949 kb on + strand at 490.030 kb on + strand, within Atu5500 at 490.044 kb on - strand, within Atu5500 at 490.102 kb on - strand, within Atu5500 at 490.189 kb on + strand, within Atu5500 at 490.269 kb on + strand, within Atu5500 at 490.361 kb on + strand, within Atu5500 at 490.369 kb on - strand, within Atu5500 at 490.376 kb on + strand, within Atu5500 at 490.381 kb on + strand, within Atu5500 at 490.389 kb on - strand, within Atu5500 at 490.408 kb on + strand, within Atu5500 at 490.416 kb on - strand, within Atu5500 at 490.437 kb on - strand, within Atu5500 at 490.450 kb on - strand, within Atu5500 at 490.533 kb on + strand, within Atu5500 at 490.539 kb on - strand, within Atu5500 at 490.591 kb on + strand, within Atu5500 at 490.596 kb on + strand, within Atu5500 at 490.608 kb on + strand, within Atu5500 at 490.662 kb on + strand, within Atu5500 at 490.670 kb on - strand, within Atu5500 at 490.687 kb on + strand, within Atu5500 at 490.722 kb on - strand, within Atu5500 at 490.729 kb on - strand, within Atu5500 at 490.745 kb on + strand, within Atu5500 at 490.765 kb on + strand, within Atu5500 at 490.775 kb on + strand, within Atu5500 at 490.817 kb on + strand, within Atu5500 at 490.937 kb on + strand, within Atu5500 at 490.988 kb on + strand, within Atu5500 at 491.077 kb on - strand, within Atu5500 at 491.090 kb on + strand, within Atu5500 at 491.192 kb on + strand, within Atu5500 at 491.200 kb on - strand, within Atu5500 at 491.218 kb on + strand, within Atu5500 at 491.225 kb on + strand, within Atu5500 at 491.243 kb on - strand, within Atu5500 at 491.243 kb on - strand, within Atu5500 at 491.412 kb on - strand, within Atu5500 at 491.477 kb on + strand, within Atu5500 at 491.513 kb on - strand, within Atu5500 at 491.621 kb on - strand, within Atu5500 at 491.641 kb on - strand, within Atu5500 at 491.643 kb on + strand, within Atu5500 at 491.658 kb on + strand, within Atu5500 at 491.666 kb on - strand, within Atu5500 at 491.677 kb on + strand, within Atu5500 at 491.699 kb on + strand, within Atu5500 at 491.756 kb on + strand, within Atu5500 at 491.756 kb on + strand, within Atu5500 at 491.764 kb on - strand, within Atu5500 at 491.764 kb on - strand, within Atu5500 at 491.766 kb on + strand, within Atu5500 at 491.785 kb on + strand, within Atu5500 at 491.843 kb on - strand at 491.868 kb on + strand at 491.933 kb on - strand at 491.935 kb on - strand at 491.942 kb on - strand at 492.044 kb on - strand at 492.062 kb on + strand at 492.066 kb on - strand at 492.088 kb on + strand at 492.123 kb on - strand at 492.181 kb on - strand at 492.227 kb on - strand at 492.232 kb on - strand at 492.252 kb on + strand at 492.276 kb on - strand at 492.324 kb on + strand at 492.332 kb on - strand at 492.410 kb on + strand at 492.454 kb on - strand at 492.637 kb on + strand, within Atu5501 at 492.645 kb on - strand, within Atu5501 at 492.664 kb on - strand, within Atu5501 at 492.833 kb on + strand, within Atu5501 at 492.992 kb on + strand, within Atu5501 at 493.042 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 12.5 ug/mL remove 488,777 - -0.2 488,862 + +0.8 488,900 + Atu5499 0.12 +1.9 488,908 - Atu5499 0.13 -1.2 488,935 + Atu5499 0.16 +2.4 488,943 - Atu5499 0.17 +2.2 488,943 - Atu5499 0.17 -0.3 488,977 - Atu5499 0.20 +0.6 488,984 + Atu5499 0.21 -1.7 488,989 + Atu5499 0.21 -0.1 488,997 - Atu5499 0.22 +0.8 489,002 - Atu5499 0.23 -1.4 489,002 - Atu5499 0.23 -0.4 489,030 + Atu5499 0.25 -2.1 489,056 - Atu5499 0.28 +0.4 489,060 + Atu5499 0.28 +0.4 489,243 - Atu5499 0.47 +0.2 489,260 + Atu5499 0.49 -1.2 489,291 + Atu5499 0.52 -0.4 489,481 + Atu5499 0.71 -1.9 489,481 + Atu5499 0.71 -0.7 489,489 - Atu5499 0.72 -0.1 489,525 + Atu5499 0.75 +0.4 489,527 - Atu5499 0.76 -0.5 489,540 + Atu5499 0.77 -0.7 489,736 + +0.9 489,833 - +0.8 489,833 - +0.6 489,869 + +0.6 489,870 + +1.0 489,871 + +0.3 489,878 - -0.0 489,878 - +0.1 489,878 - +1.6 489,879 - -0.4 489,949 + +0.3 490,030 + Atu5500 0.12 +0.2 490,044 - Atu5500 0.12 +0.3 490,102 - Atu5500 0.15 +0.2 490,189 + Atu5500 0.18 +0.3 490,269 + Atu5500 0.22 +1.1 490,361 + Atu5500 0.26 -0.0 490,369 - Atu5500 0.26 -2.1 490,376 + Atu5500 0.27 +0.4 490,381 + Atu5500 0.27 -0.2 490,389 - Atu5500 0.27 -0.7 490,408 + Atu5500 0.28 -0.6 490,416 - Atu5500 0.28 +0.3 490,437 - Atu5500 0.29 +0.6 490,450 - Atu5500 0.30 +0.5 490,533 + Atu5500 0.33 +0.4 490,539 - Atu5500 0.34 +0.6 490,591 + Atu5500 0.36 -0.4 490,596 + Atu5500 0.36 +0.3 490,608 + Atu5500 0.37 +1.1 490,662 + Atu5500 0.39 -1.2 490,670 - Atu5500 0.39 +0.5 490,687 + Atu5500 0.40 +1.6 490,722 - Atu5500 0.42 -0.0 490,729 - Atu5500 0.42 +1.6 490,745 + Atu5500 0.43 -0.5 490,765 + Atu5500 0.43 +1.5 490,775 + Atu5500 0.44 -1.6 490,817 + Atu5500 0.46 +0.6 490,937 + Atu5500 0.51 +1.7 490,988 + Atu5500 0.53 +0.3 491,077 - Atu5500 0.57 +0.3 491,090 + Atu5500 0.58 +0.6 491,192 + Atu5500 0.62 +0.0 491,200 - Atu5500 0.62 -0.7 491,218 + Atu5500 0.63 -1.0 491,225 + Atu5500 0.63 -0.4 491,243 - Atu5500 0.64 +1.3 491,243 - Atu5500 0.64 +0.5 491,412 - Atu5500 0.71 +0.9 491,477 + Atu5500 0.74 +0.3 491,513 - Atu5500 0.76 +1.1 491,621 - Atu5500 0.81 -0.1 491,641 - Atu5500 0.81 -1.2 491,643 + Atu5500 0.82 -1.7 491,658 + Atu5500 0.82 +0.3 491,666 - Atu5500 0.83 -0.1 491,677 + Atu5500 0.83 +0.4 491,699 + Atu5500 0.84 +1.2 491,756 + Atu5500 0.86 +1.1 491,756 + Atu5500 0.86 -2.2 491,764 - Atu5500 0.87 +0.1 491,764 - Atu5500 0.87 -0.2 491,766 + Atu5500 0.87 +0.2 491,785 + Atu5500 0.88 +0.8 491,843 - +2.0 491,868 + -1.0 491,933 - +0.5 491,935 - -0.9 491,942 - +0.2 492,044 - -0.3 492,062 + +1.2 492,066 - -1.0 492,088 + +0.6 492,123 - -0.1 492,181 - +0.3 492,227 - -0.2 492,232 - -0.3 492,252 + +1.1 492,276 - +0.3 492,324 + +0.9 492,332 - -0.2 492,410 + -0.7 492,454 - -0.7 492,637 + Atu5501 0.34 +0.3 492,645 - Atu5501 0.35 -0.2 492,664 - Atu5501 0.38 +1.6 492,833 + Atu5501 0.63 -0.8 492,992 + Atu5501 0.87 -0.6 493,042 + -1.0
Or see this region's nucleotide sequence