Experiment: Chloramphenicol 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5160 and Atu5161 are separated by 93 nucleotides Atu5161 and Atu5162 are separated by 2 nucleotides Atu5162 and Atu5163 overlap by 4 nucleotides Atu5163 and Atu5164 are separated by 2 nucleotides Atu5164 and Atu5165 are separated by 100 nucleotides
Atu5160: Atu5160 - transcriptional regulator protein, at 159,510 to 159,872
Atu5160
Atu5161: Atu5161 - hypothetical protein, at 159,966 to 160,505
Atu5161
Atu5162: Atu5162 - type IV secretion protein AvhB1, at 160,508 to 161,194
Atu5162
Atu5163: Atu5163 - type IV secretion protein AvhB2, at 161,191 to 161,490
Atu5163
Atu5164: Atu5164 - type IV secretion protein AvhB3, at 161,493 to 161,834
Atu5164
Atu5165: Atu5165 - type IV secretion protein AvhB4, at 161,935 to 164,208
Atu5165
Position (kb)
160
161
162 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 159.543 kb on - strand at 159.655 kb on - strand, within Atu5160 at 159.766 kb on + strand, within Atu5160 at 159.833 kb on + strand, within Atu5160 at 159.833 kb on + strand, within Atu5160 at 159.842 kb on - strand at 159.953 kb on - strand at 160.006 kb on + strand at 160.066 kb on + strand, within Atu5161 at 160.074 kb on - strand, within Atu5161 at 160.079 kb on - strand, within Atu5161 at 160.096 kb on + strand, within Atu5161 at 160.136 kb on + strand, within Atu5161 at 160.181 kb on + strand, within Atu5161 at 160.206 kb on - strand, within Atu5161 at 160.342 kb on + strand, within Atu5161 at 160.387 kb on + strand, within Atu5161 at 160.395 kb on - strand, within Atu5161 at 160.420 kb on - strand, within Atu5161 at 160.420 kb on - strand, within Atu5161 at 160.431 kb on + strand, within Atu5161 at 160.449 kb on + strand, within Atu5161 at 160.492 kb on + strand at 160.492 kb on + strand at 160.507 kb on - strand at 160.511 kb on + strand at 160.547 kb on - strand at 160.621 kb on + strand, within Atu5162 at 160.656 kb on + strand, within Atu5162 at 160.656 kb on + strand, within Atu5162 at 160.664 kb on - strand, within Atu5162 at 160.664 kb on - strand, within Atu5162 at 160.747 kb on - strand, within Atu5162 at 160.749 kb on + strand, within Atu5162 at 160.790 kb on + strand, within Atu5162 at 160.849 kb on + strand, within Atu5162 at 160.968 kb on - strand, within Atu5162 at 161.128 kb on - strand at 161.129 kb on + strand at 161.208 kb on + strand at 161.399 kb on + strand, within Atu5163 at 161.404 kb on + strand, within Atu5163 at 161.404 kb on + strand, within Atu5163 at 161.412 kb on - strand, within Atu5163 at 161.484 kb on + strand at 161.618 kb on - strand, within Atu5164 at 161.843 kb on + strand at 161.924 kb on + strand at 161.980 kb on - strand at 162.077 kb on + strand at 162.077 kb on + strand at 162.085 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 12.5 ug/mL remove 159,543 - -0.4 159,655 - Atu5160 0.40 +1.6 159,766 + Atu5160 0.71 -0.8 159,833 + Atu5160 0.89 -0.4 159,833 + Atu5160 0.89 -0.7 159,842 - -1.1 159,953 - +2.7 160,006 + -1.2 160,066 + Atu5161 0.19 +0.3 160,074 - Atu5161 0.20 +0.6 160,079 - Atu5161 0.21 -1.5 160,096 + Atu5161 0.24 -1.0 160,136 + Atu5161 0.31 +0.4 160,181 + Atu5161 0.40 -0.2 160,206 - Atu5161 0.44 -1.2 160,342 + Atu5161 0.70 +1.1 160,387 + Atu5161 0.78 +1.3 160,395 - Atu5161 0.79 +0.5 160,420 - Atu5161 0.84 -1.0 160,420 - Atu5161 0.84 -0.7 160,431 + Atu5161 0.86 -0.4 160,449 + Atu5161 0.89 -0.1 160,492 + +0.7 160,492 + +0.0 160,507 - -2.5 160,511 + +0.7 160,547 - +1.6 160,621 + Atu5162 0.16 +0.8 160,656 + Atu5162 0.22 -1.4 160,656 + Atu5162 0.22 +0.6 160,664 - Atu5162 0.23 +1.0 160,664 - Atu5162 0.23 +0.8 160,747 - Atu5162 0.35 +1.0 160,749 + Atu5162 0.35 +0.4 160,790 + Atu5162 0.41 +0.3 160,849 + Atu5162 0.50 +0.2 160,968 - Atu5162 0.67 +0.8 161,128 - +2.4 161,129 + +1.1 161,208 + +1.5 161,399 + Atu5163 0.69 +1.8 161,404 + Atu5163 0.71 +0.4 161,404 + Atu5163 0.71 +0.8 161,412 - Atu5163 0.74 +2.0 161,484 + -0.4 161,618 - Atu5164 0.37 -0.9 161,843 + +0.5 161,924 + +0.5 161,980 - -0.1 162,077 + -1.6 162,077 + -0.3 162,085 - +0.2
Or see this region's nucleotide sequence