Experiment: Chloramphenicol 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4220 and Atu4221 are separated by 23 nucleotides Atu4221 and Atu4222 overlap by 8 nucleotides Atu4222 and Atu4223 overlap by 4 nucleotides Atu4223 and Atu4224 are separated by 24 nucleotides
Atu4220: Atu4220 - short-chain dehydrogenase, at 1,344,211 to 1,345,086
Atu4220
Atu4221: Atu4221 - hypothetical protein, at 1,345,110 to 1,345,658
Atu4221
Atu4222: Atu4222 - sarcosine oxidase alpha subunit, at 1,345,651 to 1,348,614
Atu4222
Atu4223: Atu4223 - sarcosine oxidase delta subunit, at 1,348,611 to 1,348,886
Atu4223
Atu4224: Atu4224 - sarcosine oxidase beta subunit, at 1,348,911 to 1,350,161
Atu4224
Position (kb)
1345
1346
1347
1348
1349 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1344.676 kb on - strand, within Atu4220 at 1344.748 kb on + strand, within Atu4220 at 1344.748 kb on + strand, within Atu4220 at 1344.748 kb on + strand, within Atu4220 at 1344.748 kb on + strand, within Atu4220 at 1344.756 kb on - strand, within Atu4220 at 1344.756 kb on - strand, within Atu4220 at 1344.756 kb on - strand, within Atu4220 at 1344.756 kb on - strand, within Atu4220 at 1344.842 kb on + strand, within Atu4220 at 1344.857 kb on - strand, within Atu4220 at 1344.874 kb on + strand, within Atu4220 at 1344.895 kb on + strand, within Atu4220 at 1344.903 kb on - strand, within Atu4220 at 1344.908 kb on - strand, within Atu4220 at 1344.913 kb on - strand, within Atu4220 at 1345.086 kb on + strand at 1345.094 kb on - strand at 1345.278 kb on - strand, within Atu4221 at 1345.283 kb on + strand, within Atu4221 at 1345.355 kb on + strand, within Atu4221 at 1345.408 kb on - strand, within Atu4221 at 1345.419 kb on - strand, within Atu4221 at 1345.422 kb on - strand, within Atu4221 at 1345.440 kb on + strand, within Atu4221 at 1345.524 kb on - strand, within Atu4221 at 1345.529 kb on - strand, within Atu4221 at 1345.529 kb on - strand, within Atu4221 at 1345.529 kb on - strand, within Atu4221 at 1345.571 kb on - strand, within Atu4221 at 1345.596 kb on + strand, within Atu4221 at 1345.732 kb on + strand at 1345.741 kb on - strand at 1345.821 kb on + strand at 1345.840 kb on - strand at 1345.844 kb on + strand at 1346.075 kb on - strand, within Atu4222 at 1346.081 kb on + strand, within Atu4222 at 1346.172 kb on + strand, within Atu4222 at 1346.225 kb on + strand, within Atu4222 at 1346.414 kb on - strand, within Atu4222 at 1346.464 kb on + strand, within Atu4222 at 1346.547 kb on + strand, within Atu4222 at 1346.555 kb on - strand, within Atu4222 at 1346.555 kb on - strand, within Atu4222 at 1346.692 kb on + strand, within Atu4222 at 1346.700 kb on - strand, within Atu4222 at 1346.728 kb on + strand, within Atu4222 at 1346.996 kb on + strand, within Atu4222 at 1347.011 kb on + strand, within Atu4222 at 1347.017 kb on + strand, within Atu4222 at 1347.020 kb on - strand, within Atu4222 at 1347.036 kb on + strand, within Atu4222 at 1347.037 kb on + strand, within Atu4222 at 1347.037 kb on + strand, within Atu4222 at 1347.043 kb on + strand, within Atu4222 at 1347.045 kb on - strand, within Atu4222 at 1347.045 kb on - strand, within Atu4222 at 1347.069 kb on - strand, within Atu4222 at 1347.069 kb on - strand, within Atu4222 at 1347.243 kb on + strand, within Atu4222 at 1347.243 kb on + strand, within Atu4222 at 1347.243 kb on + strand, within Atu4222 at 1347.243 kb on + strand, within Atu4222 at 1347.243 kb on + strand, within Atu4222 at 1347.251 kb on - strand, within Atu4222 at 1347.251 kb on - strand, within Atu4222 at 1347.251 kb on - strand, within Atu4222 at 1347.276 kb on + strand, within Atu4222 at 1347.289 kb on - strand, within Atu4222 at 1347.336 kb on + strand, within Atu4222 at 1347.336 kb on + strand, within Atu4222 at 1347.349 kb on - strand, within Atu4222 at 1347.532 kb on + strand, within Atu4222 at 1347.532 kb on + strand, within Atu4222 at 1347.606 kb on + strand, within Atu4222 at 1347.614 kb on - strand, within Atu4222 at 1347.621 kb on + strand, within Atu4222 at 1347.685 kb on - strand, within Atu4222 at 1347.757 kb on + strand, within Atu4222 at 1347.793 kb on - strand, within Atu4222 at 1347.823 kb on + strand, within Atu4222 at 1347.831 kb on - strand, within Atu4222 at 1347.851 kb on - strand, within Atu4222 at 1347.913 kb on + strand, within Atu4222 at 1347.919 kb on + strand, within Atu4222 at 1347.925 kb on + strand, within Atu4222 at 1348.165 kb on - strand, within Atu4222 at 1348.225 kb on + strand, within Atu4222 at 1348.251 kb on + strand, within Atu4222 at 1348.262 kb on + strand, within Atu4222 at 1348.348 kb on - strand at 1348.372 kb on - strand at 1348.391 kb on - strand at 1348.500 kb on + strand at 1348.500 kb on + strand at 1348.500 kb on + strand at 1348.528 kb on + strand at 1348.566 kb on + strand at 1348.668 kb on + strand, within Atu4223 at 1348.696 kb on - strand, within Atu4223 at 1348.877 kb on - strand at 1348.877 kb on - strand at 1348.908 kb on + strand at 1348.926 kb on - strand at 1348.988 kb on + strand at 1349.057 kb on + strand, within Atu4224 at 1349.088 kb on + strand, within Atu4224 at 1349.116 kb on - strand, within Atu4224 at 1349.116 kb on - strand, within Atu4224 at 1349.129 kb on - strand, within Atu4224 at 1349.284 kb on + strand, within Atu4224 at 1349.330 kb on - strand, within Atu4224 at 1349.371 kb on - strand, within Atu4224 at 1349.396 kb on - strand, within Atu4224 at 1349.401 kb on - strand, within Atu4224 at 1349.439 kb on - strand, within Atu4224 at 1349.449 kb on - strand, within Atu4224 at 1349.548 kb on - strand, within Atu4224 at 1349.553 kb on + strand, within Atu4224 at 1349.561 kb on - strand, within Atu4224 at 1349.579 kb on + strand, within Atu4224 at 1349.598 kb on + strand, within Atu4224 at 1349.607 kb on - strand, within Atu4224
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 12.5 ug/mL remove 1,344,676 - Atu4220 0.53 -0.4 1,344,748 + Atu4220 0.61 +2.5 1,344,748 + Atu4220 0.61 +0.1 1,344,748 + Atu4220 0.61 +0.2 1,344,748 + Atu4220 0.61 -0.8 1,344,756 - Atu4220 0.62 +1.5 1,344,756 - Atu4220 0.62 +1.1 1,344,756 - Atu4220 0.62 +0.1 1,344,756 - Atu4220 0.62 -0.8 1,344,842 + Atu4220 0.72 -1.5 1,344,857 - Atu4220 0.74 -0.1 1,344,874 + Atu4220 0.76 -1.3 1,344,895 + Atu4220 0.78 -1.3 1,344,903 - Atu4220 0.79 -0.1 1,344,908 - Atu4220 0.80 -0.3 1,344,913 - Atu4220 0.80 +1.6 1,345,086 + +0.5 1,345,094 - -0.3 1,345,278 - Atu4221 0.31 -0.1 1,345,283 + Atu4221 0.32 +0.1 1,345,355 + Atu4221 0.45 +1.4 1,345,408 - Atu4221 0.54 +0.7 1,345,419 - Atu4221 0.56 -0.1 1,345,422 - Atu4221 0.57 -0.6 1,345,440 + Atu4221 0.60 +1.9 1,345,524 - Atu4221 0.75 -2.0 1,345,529 - Atu4221 0.76 -0.3 1,345,529 - Atu4221 0.76 -0.5 1,345,529 - Atu4221 0.76 +1.5 1,345,571 - Atu4221 0.84 +0.7 1,345,596 + Atu4221 0.89 +0.7 1,345,732 + -0.5 1,345,741 - +0.7 1,345,821 + -0.0 1,345,840 - +0.1 1,345,844 + +1.0 1,346,075 - Atu4222 0.14 +0.9 1,346,081 + Atu4222 0.15 +0.3 1,346,172 + Atu4222 0.18 +0.2 1,346,225 + Atu4222 0.19 -0.5 1,346,414 - Atu4222 0.26 -0.9 1,346,464 + Atu4222 0.27 +0.1 1,346,547 + Atu4222 0.30 -0.6 1,346,555 - Atu4222 0.30 +1.1 1,346,555 - Atu4222 0.30 -1.7 1,346,692 + Atu4222 0.35 +1.4 1,346,700 - Atu4222 0.35 +1.5 1,346,728 + Atu4222 0.36 -1.3 1,346,996 + Atu4222 0.45 +0.2 1,347,011 + Atu4222 0.46 -1.7 1,347,017 + Atu4222 0.46 +0.2 1,347,020 - Atu4222 0.46 -0.6 1,347,036 + Atu4222 0.47 +0.8 1,347,037 + Atu4222 0.47 -0.8 1,347,037 + Atu4222 0.47 +0.1 1,347,043 + Atu4222 0.47 -0.5 1,347,045 - Atu4222 0.47 +0.7 1,347,045 - Atu4222 0.47 -1.7 1,347,069 - Atu4222 0.48 -0.4 1,347,069 - Atu4222 0.48 -0.1 1,347,243 + Atu4222 0.54 +1.5 1,347,243 + Atu4222 0.54 +0.4 1,347,243 + Atu4222 0.54 +0.4 1,347,243 + Atu4222 0.54 -1.1 1,347,243 + Atu4222 0.54 +1.5 1,347,251 - Atu4222 0.54 +0.2 1,347,251 - Atu4222 0.54 +0.7 1,347,251 - Atu4222 0.54 -0.3 1,347,276 + Atu4222 0.55 +0.9 1,347,289 - Atu4222 0.55 -0.1 1,347,336 + Atu4222 0.57 +1.0 1,347,336 + Atu4222 0.57 -0.3 1,347,349 - Atu4222 0.57 -0.3 1,347,532 + Atu4222 0.63 -0.7 1,347,532 + Atu4222 0.63 +0.2 1,347,606 + Atu4222 0.66 -1.3 1,347,614 - Atu4222 0.66 +0.8 1,347,621 + Atu4222 0.66 +0.8 1,347,685 - Atu4222 0.69 +0.4 1,347,757 + Atu4222 0.71 +0.8 1,347,793 - Atu4222 0.72 -2.2 1,347,823 + Atu4222 0.73 +0.8 1,347,831 - Atu4222 0.74 +2.6 1,347,851 - Atu4222 0.74 -0.0 1,347,913 + Atu4222 0.76 -0.4 1,347,919 + Atu4222 0.77 +0.1 1,347,925 + Atu4222 0.77 -0.3 1,348,165 - Atu4222 0.85 -0.6 1,348,225 + Atu4222 0.87 -2.0 1,348,251 + Atu4222 0.88 +0.2 1,348,262 + Atu4222 0.88 -2.6 1,348,348 - +0.2 1,348,372 - -2.5 1,348,391 - -1.8 1,348,500 + +0.2 1,348,500 + +0.9 1,348,500 + +1.3 1,348,528 + +1.5 1,348,566 + +0.7 1,348,668 + Atu4223 0.21 +1.5 1,348,696 - Atu4223 0.31 -1.3 1,348,877 - +0.1 1,348,877 - +1.2 1,348,908 + +0.5 1,348,926 - -1.2 1,348,988 + +0.9 1,349,057 + Atu4224 0.12 -0.1 1,349,088 + Atu4224 0.14 -2.5 1,349,116 - Atu4224 0.16 -2.1 1,349,116 - Atu4224 0.16 -0.1 1,349,129 - Atu4224 0.17 +0.6 1,349,284 + Atu4224 0.30 -0.8 1,349,330 - Atu4224 0.33 -0.8 1,349,371 - Atu4224 0.37 +0.2 1,349,396 - Atu4224 0.39 +1.5 1,349,401 - Atu4224 0.39 +0.5 1,349,439 - Atu4224 0.42 -0.1 1,349,449 - Atu4224 0.43 -2.3 1,349,548 - Atu4224 0.51 +0.3 1,349,553 + Atu4224 0.51 +0.7 1,349,561 - Atu4224 0.52 -0.8 1,349,579 + Atu4224 0.53 -1.1 1,349,598 + Atu4224 0.55 +1.4 1,349,607 - Atu4224 0.56 +0.6
Or see this region's nucleotide sequence