Experiment: Chloramphenicol 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4063 and Atu4064 are separated by 135 nucleotides Atu4064 and Atu4065 are separated by 1 nucleotides Atu4065 and Atu4066 are separated by 96 nucleotides Atu4066 and Atu4067 are separated by 14 nucleotides Atu4067 and Atu4068 are separated by 11 nucleotides Atu4068 and Atu4069 overlap by 4 nucleotides
Atu4063: Atu4063 - O-linked GlcNAc transferase, at 1,180,552 to 1,181,448
Atu4063
Atu4064: Atu4064 - 30S ribosomal protein S21, at 1,181,584 to 1,181,811
Atu4064
Atu4065: Atu4065 - hypothetical protein, at 1,181,813 to 1,182,031
Atu4065
Atu4066: Atu4066 - sarcosine oxidase beta subunit, at 1,182,128 to 1,183,381
Atu4066
Atu4067: Atu4067 - hypothetical protein, at 1,183,396 to 1,183,791
Atu4067
Atu4068: Atu4068 - sarcosine oxidase delta subunit, at 1,183,803 to 1,184,117
Atu4068
Atu4069: Atu4069 - sarcosine oxidase alpha subunit, at 1,184,114 to 1,187,074
Atu4069
Position (kb)
1182
1183
1184 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1181.138 kb on + strand, within Atu4063 at 1181.146 kb on - strand, within Atu4063 at 1181.179 kb on + strand, within Atu4063 at 1181.187 kb on - strand, within Atu4063 at 1181.207 kb on - strand, within Atu4063 at 1181.243 kb on - strand, within Atu4063 at 1181.277 kb on - strand, within Atu4063 at 1181.345 kb on + strand, within Atu4063 at 1181.351 kb on - strand, within Atu4063 at 1181.353 kb on - strand, within Atu4063 at 1181.361 kb on - strand at 1181.380 kb on - strand at 1181.623 kb on - strand, within Atu4064 at 1182.064 kb on + strand at 1182.064 kb on + strand at 1182.064 kb on + strand at 1182.076 kb on + strand at 1182.076 kb on + strand at 1182.086 kb on + strand at 1182.199 kb on + strand at 1182.223 kb on + strand at 1182.269 kb on + strand, within Atu4066 at 1182.269 kb on + strand, within Atu4066 at 1182.269 kb on + strand, within Atu4066 at 1182.337 kb on + strand, within Atu4066 at 1182.359 kb on + strand, within Atu4066 at 1182.431 kb on + strand, within Atu4066 at 1182.583 kb on + strand, within Atu4066 at 1182.591 kb on - strand, within Atu4066 at 1182.634 kb on + strand, within Atu4066 at 1182.689 kb on + strand, within Atu4066 at 1182.697 kb on - strand, within Atu4066 at 1182.710 kb on + strand, within Atu4066 at 1182.710 kb on + strand, within Atu4066 at 1182.718 kb on - strand, within Atu4066 at 1182.734 kb on + strand, within Atu4066 at 1182.742 kb on - strand, within Atu4066 at 1182.878 kb on - strand, within Atu4066 at 1182.923 kb on - strand, within Atu4066 at 1182.924 kb on - strand, within Atu4066 at 1182.948 kb on + strand, within Atu4066 at 1182.961 kb on - strand, within Atu4066 at 1182.968 kb on + strand, within Atu4066 at 1182.978 kb on + strand, within Atu4066 at 1183.030 kb on + strand, within Atu4066 at 1183.063 kb on - strand, within Atu4066 at 1183.124 kb on - strand, within Atu4066 at 1183.148 kb on + strand, within Atu4066 at 1183.153 kb on + strand, within Atu4066 at 1183.153 kb on + strand, within Atu4066 at 1183.242 kb on - strand, within Atu4066 at 1183.289 kb on + strand at 1183.327 kb on + strand at 1183.327 kb on + strand at 1183.335 kb on - strand at 1183.386 kb on + strand at 1183.418 kb on - strand at 1183.450 kb on + strand, within Atu4067 at 1183.521 kb on - strand, within Atu4067 at 1183.605 kb on + strand, within Atu4067 at 1183.613 kb on - strand, within Atu4067 at 1183.613 kb on - strand, within Atu4067 at 1183.614 kb on - strand, within Atu4067 at 1183.665 kb on + strand, within Atu4067 at 1183.675 kb on + strand, within Atu4067 at 1183.702 kb on + strand, within Atu4067 at 1183.838 kb on - strand, within Atu4068 at 1183.866 kb on + strand, within Atu4068 at 1184.046 kb on + strand, within Atu4068 at 1184.158 kb on + strand at 1184.158 kb on + strand at 1184.244 kb on - strand at 1184.244 kb on - strand at 1184.366 kb on - strand at 1184.378 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 12.5 ug/mL remove 1,181,138 + Atu4063 0.65 -1.2 1,181,146 - Atu4063 0.66 +1.5 1,181,179 + Atu4063 0.70 -0.4 1,181,187 - Atu4063 0.71 -0.6 1,181,207 - Atu4063 0.73 -0.4 1,181,243 - Atu4063 0.77 +0.9 1,181,277 - Atu4063 0.81 -0.7 1,181,345 + Atu4063 0.88 -1.6 1,181,351 - Atu4063 0.89 -1.2 1,181,353 - Atu4063 0.89 -0.3 1,181,361 - -0.7 1,181,380 - -1.8 1,181,623 - Atu4064 0.17 +3.1 1,182,064 + -0.5 1,182,064 + -0.4 1,182,064 + -0.4 1,182,076 + +0.2 1,182,076 + +1.9 1,182,086 + -0.7 1,182,199 + +0.4 1,182,223 + -0.2 1,182,269 + Atu4066 0.11 -1.1 1,182,269 + Atu4066 0.11 -1.3 1,182,269 + Atu4066 0.11 +0.2 1,182,337 + Atu4066 0.17 -0.4 1,182,359 + Atu4066 0.18 +0.7 1,182,431 + Atu4066 0.24 +1.3 1,182,583 + Atu4066 0.36 -0.2 1,182,591 - Atu4066 0.37 +3.2 1,182,634 + Atu4066 0.40 -0.7 1,182,689 + Atu4066 0.45 -1.8 1,182,697 - Atu4066 0.45 +0.1 1,182,710 + Atu4066 0.46 +0.5 1,182,710 + Atu4066 0.46 -0.2 1,182,718 - Atu4066 0.47 -0.9 1,182,734 + Atu4066 0.48 -0.4 1,182,742 - Atu4066 0.49 -0.6 1,182,878 - Atu4066 0.60 -1.1 1,182,923 - Atu4066 0.63 -1.0 1,182,924 - Atu4066 0.63 -0.8 1,182,948 + Atu4066 0.65 -0.5 1,182,961 - Atu4066 0.66 -0.5 1,182,968 + Atu4066 0.67 -3.0 1,182,978 + Atu4066 0.68 -0.3 1,183,030 + Atu4066 0.72 +0.5 1,183,063 - Atu4066 0.75 -1.5 1,183,124 - Atu4066 0.79 +0.0 1,183,148 + Atu4066 0.81 +1.2 1,183,153 + Atu4066 0.82 -1.1 1,183,153 + Atu4066 0.82 -0.7 1,183,242 - Atu4066 0.89 -0.3 1,183,289 + -0.0 1,183,327 + -2.2 1,183,327 + -1.9 1,183,335 - -1.1 1,183,386 + -0.7 1,183,418 - +0.3 1,183,450 + Atu4067 0.14 +0.7 1,183,521 - Atu4067 0.32 -1.1 1,183,605 + Atu4067 0.53 -0.0 1,183,613 - Atu4067 0.55 -0.5 1,183,613 - Atu4067 0.55 -0.3 1,183,614 - Atu4067 0.55 -1.0 1,183,665 + Atu4067 0.68 -0.2 1,183,675 + Atu4067 0.70 +0.1 1,183,702 + Atu4067 0.77 -0.4 1,183,838 - Atu4068 0.11 +0.2 1,183,866 + Atu4068 0.20 -0.5 1,184,046 + Atu4068 0.77 +2.0 1,184,158 + +0.0 1,184,158 + +0.1 1,184,244 - -0.1 1,184,244 - -0.4 1,184,366 - +0.1 1,184,378 + +1.3
Or see this region's nucleotide sequence