Experiment: Chloramphenicol 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3601 and Atu3602 are separated by 166 nucleotides Atu3602 and Atu8114 are separated by 184 nucleotides Atu8114 and Atu3603 are separated by 117 nucleotides
Atu3601: Atu3601 - diaminopimelate decarboxylase, at 654,822 to 656,090
Atu3601
Atu3602: Atu3602 - hypothetical protein, at 656,257 to 658,965
Atu3602
Atu8114: Atu8114 - hypothetical protein, at 659,150 to 659,356
Atu8114
Atu3603: Atu3603 - two component response regulator, at 659,474 to 659,833
Atu3603
Position (kb) 656
657
658
659 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 656.100 kb on + strand at 656.160 kb on - strand at 656.160 kb on - strand at 656.182 kb on - strand at 656.233 kb on + strand at 656.241 kb on - strand at 659.120 kb on + strand at 659.381 kb on + strand at 659.386 kb on + strand at 659.391 kb on + strand at 659.399 kb on - strand at 659.406 kb on - strand at 659.457 kb on - strand at 659.459 kb on + strand at 659.499 kb on + strand at 659.518 kb on + strand, within Atu3603 at 659.581 kb on + strand, within Atu3603 at 659.589 kb on - strand, within Atu3603 at 659.752 kb on + strand, within Atu3603 at 659.875 kb on + strand at 659.928 kb on - strand at 659.958 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 12.5 ug/mL remove 656,100 + +0.6 656,160 - +0.7 656,160 - -0.2 656,182 - -1.1 656,233 + +0.7 656,241 - -1.6 659,120 + +1.9 659,381 + +0.6 659,386 + -1.5 659,391 + -0.6 659,399 - -0.1 659,406 - +0.4 659,457 - -0.1 659,459 + -0.3 659,499 + -1.0 659,518 + Atu3603 0.12 +3.5 659,581 + Atu3603 0.30 +1.4 659,589 - Atu3603 0.32 -1.0 659,752 + Atu3603 0.77 +0.4 659,875 + -1.7 659,928 - -0.4 659,958 - -1.9
Or see this region's nucleotide sequence