Experiment: Chloramphenicol 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3479 and Atu3480 are separated by 1 nucleotides Atu3480 and Atu3481 overlap by 11 nucleotides Atu3481 and Atu3482 are separated by 140 nucleotides
Atu3479: Atu3479 - 3-methylcrotonyl-CoA carboxylase subunit alpha, at 527,314 to 529,305
Atu3479
Atu3480: Atu3480 - hydroxymethylglutaryl-CoA lyase, at 529,307 to 530,233
Atu3480
Atu3481: Atu3481 - enoyl-CoA hydratase, at 530,223 to 530,999
Atu3481
Atu3482: Atu3482 - pantoate--beta-alanine ligase, at 531,140 to 532,018
Atu3482
Position (kb) 530
531 Strain fitness (log2 ratio) -2
-1
0
1
2 at 529.268 kb on + strand at 529.276 kb on - strand at 529.447 kb on - strand, within Atu3480 at 529.447 kb on - strand, within Atu3480 at 529.518 kb on - strand, within Atu3480 at 529.530 kb on - strand, within Atu3480 at 529.672 kb on - strand, within Atu3480 at 529.693 kb on + strand, within Atu3480 at 529.816 kb on - strand, within Atu3480 at 529.877 kb on + strand, within Atu3480 at 529.938 kb on + strand, within Atu3480 at 529.938 kb on + strand, within Atu3480 at 529.982 kb on + strand, within Atu3480 at 530.036 kb on - strand, within Atu3480 at 530.206 kb on + strand at 530.493 kb on + strand, within Atu3481 at 530.520 kb on + strand, within Atu3481 at 530.533 kb on + strand, within Atu3481 at 530.703 kb on - strand, within Atu3481 at 530.737 kb on + strand, within Atu3481 at 530.786 kb on + strand, within Atu3481 at 530.993 kb on + strand at 531.060 kb on - strand at 531.092 kb on + strand at 531.223 kb on + strand at 531.276 kb on - strand, within Atu3482 at 531.422 kb on - strand, within Atu3482 at 531.446 kb on + strand, within Atu3482 at 531.586 kb on + strand, within Atu3482 at 531.628 kb on - strand, within Atu3482 at 531.628 kb on - strand, within Atu3482 at 531.628 kb on - strand, within Atu3482 at 531.710 kb on - strand, within Atu3482 at 531.772 kb on + strand, within Atu3482 at 531.923 kb on - strand, within Atu3482
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 12.5 ug/mL remove 529,268 + -1.1 529,276 - +0.6 529,447 - Atu3480 0.15 +0.1 529,447 - Atu3480 0.15 +0.0 529,518 - Atu3480 0.23 +0.7 529,530 - Atu3480 0.24 -0.1 529,672 - Atu3480 0.39 +0.3 529,693 + Atu3480 0.42 -0.3 529,816 - Atu3480 0.55 -0.2 529,877 + Atu3480 0.61 -1.5 529,938 + Atu3480 0.68 -0.6 529,938 + Atu3480 0.68 -2.5 529,982 + Atu3480 0.73 -0.6 530,036 - Atu3480 0.79 -0.4 530,206 + -0.8 530,493 + Atu3481 0.35 -0.9 530,520 + Atu3481 0.38 +0.1 530,533 + Atu3481 0.40 +0.8 530,703 - Atu3481 0.62 +0.9 530,737 + Atu3481 0.66 -0.2 530,786 + Atu3481 0.72 -0.0 530,993 + +0.4 531,060 - -0.9 531,092 + -0.4 531,223 + +0.6 531,276 - Atu3482 0.15 +0.6 531,422 - Atu3482 0.32 -0.1 531,446 + Atu3482 0.35 +1.6 531,586 + Atu3482 0.51 +2.2 531,628 - Atu3482 0.56 +0.3 531,628 - Atu3482 0.56 +0.6 531,628 - Atu3482 0.56 -1.9 531,710 - Atu3482 0.65 +0.5 531,772 + Atu3482 0.72 -0.7 531,923 - Atu3482 0.89 +1.4
Or see this region's nucleotide sequence