Experiment: Chloramphenicol 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3375 and Atu3376 overlap by 4 nucleotides Atu3376 and Atu3377 are separated by 7 nucleotides Atu3377 and Atu3378 overlap by 4 nucleotides Atu3378 and Atu3379 overlap by 4 nucleotides
Atu3375: Atu3375 - peptide ABC transporter substrate-binding protein, at 416,506 to 418,083
Atu3375
Atu3376: Atu3376 - beta-lactamase, at 418,080 to 418,928
Atu3376
Atu3377: Atu3377 - peptide ABC transporter ATPase, at 418,936 to 420,570
Atu3377
Atu3378: Atu3378 - oligopeptide ABC transporter permease, at 420,567 to 421,385
Atu3378
Atu3379: Atu3379 - peptide ABC transporter permease, at 421,382 to 422,326
Atu3379
Position (kb) 418
419
420
421 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 417.950 kb on + strand at 417.960 kb on + strand at 418.052 kb on + strand at 418.052 kb on + strand at 418.060 kb on - strand at 418.060 kb on - strand at 418.060 kb on - strand at 418.060 kb on - strand at 418.085 kb on + strand at 418.085 kb on + strand at 418.085 kb on + strand at 418.085 kb on + strand at 418.085 kb on + strand at 418.085 kb on + strand at 418.093 kb on - strand at 418.106 kb on - strand at 418.186 kb on + strand, within Atu3376 at 418.186 kb on + strand, within Atu3376 at 418.249 kb on - strand, within Atu3376 at 418.264 kb on - strand, within Atu3376 at 418.378 kb on + strand, within Atu3376 at 418.404 kb on - strand, within Atu3376 at 418.475 kb on - strand, within Atu3376 at 418.576 kb on - strand, within Atu3376 at 418.578 kb on + strand, within Atu3376 at 418.586 kb on - strand, within Atu3376 at 418.621 kb on - strand, within Atu3376 at 418.661 kb on + strand, within Atu3376 at 418.709 kb on + strand, within Atu3376 at 418.756 kb on + strand, within Atu3376 at 418.842 kb on + strand, within Atu3376 at 418.850 kb on - strand at 418.886 kb on - strand at 418.905 kb on - strand at 419.034 kb on + strand at 419.034 kb on + strand at 419.042 kb on - strand at 419.133 kb on + strand, within Atu3377 at 419.135 kb on - strand, within Atu3377 at 419.243 kb on - strand, within Atu3377 at 419.323 kb on + strand, within Atu3377 at 419.328 kb on + strand, within Atu3377 at 419.336 kb on - strand, within Atu3377 at 419.341 kb on - strand, within Atu3377 at 419.343 kb on + strand, within Atu3377 at 419.349 kb on + strand, within Atu3377 at 419.349 kb on + strand, within Atu3377 at 419.349 kb on + strand, within Atu3377 at 419.351 kb on - strand, within Atu3377 at 419.405 kb on - strand, within Atu3377 at 419.431 kb on - strand, within Atu3377 at 419.455 kb on - strand, within Atu3377 at 419.455 kb on - strand, within Atu3377 at 419.476 kb on - strand, within Atu3377 at 419.497 kb on + strand, within Atu3377 at 419.602 kb on - strand, within Atu3377 at 419.698 kb on - strand, within Atu3377 at 419.766 kb on + strand, within Atu3377 at 419.774 kb on - strand, within Atu3377 at 419.799 kb on - strand, within Atu3377 at 419.847 kb on + strand, within Atu3377 at 419.860 kb on + strand, within Atu3377 at 419.906 kb on + strand, within Atu3377 at 419.918 kb on + strand, within Atu3377 at 419.938 kb on + strand, within Atu3377 at 419.988 kb on + strand, within Atu3377 at 419.988 kb on - strand, within Atu3377 at 420.068 kb on + strand, within Atu3377 at 420.290 kb on + strand, within Atu3377 at 420.305 kb on - strand, within Atu3377 at 420.340 kb on + strand, within Atu3377 at 420.394 kb on + strand, within Atu3377 at 420.494 kb on + strand at 420.547 kb on + strand at 420.577 kb on + strand at 420.590 kb on + strand at 420.598 kb on - strand at 420.676 kb on - strand, within Atu3378 at 420.746 kb on + strand, within Atu3378 at 420.754 kb on - strand, within Atu3378 at 420.762 kb on + strand, within Atu3378 at 420.832 kb on - strand, within Atu3378 at 420.923 kb on + strand, within Atu3378 at 420.923 kb on + strand, within Atu3378 at 420.926 kb on - strand, within Atu3378 at 420.931 kb on - strand, within Atu3378 at 421.019 kb on + strand, within Atu3378 at 421.231 kb on - strand, within Atu3378 at 421.281 kb on - strand, within Atu3378 at 421.300 kb on + strand, within Atu3378 at 421.300 kb on + strand, within Atu3378 at 421.305 kb on + strand at 421.308 kb on - strand at 421.308 kb on - strand at 421.318 kb on - strand at 421.329 kb on + strand at 421.377 kb on - strand at 421.413 kb on + strand at 421.421 kb on - strand at 421.421 kb on - strand at 421.483 kb on - strand, within Atu3379 at 421.488 kb on + strand, within Atu3379 at 421.541 kb on + strand, within Atu3379 at 421.541 kb on + strand, within Atu3379
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 12.5 ug/mL remove 417,950 + -1.2 417,960 + +2.0 418,052 + +0.8 418,052 + -0.3 418,060 - -0.4 418,060 - -0.5 418,060 - -2.1 418,060 - +1.1 418,085 + -0.7 418,085 + +0.6 418,085 + -1.2 418,085 + -1.6 418,085 + -0.1 418,085 + +0.6 418,093 - +0.5 418,106 - +1.0 418,186 + Atu3376 0.12 -0.9 418,186 + Atu3376 0.12 +0.0 418,249 - Atu3376 0.20 +1.3 418,264 - Atu3376 0.22 -0.8 418,378 + Atu3376 0.35 -0.4 418,404 - Atu3376 0.38 -0.7 418,475 - Atu3376 0.47 +0.1 418,576 - Atu3376 0.58 +1.2 418,578 + Atu3376 0.59 -0.9 418,586 - Atu3376 0.60 +0.3 418,621 - Atu3376 0.64 -0.3 418,661 + Atu3376 0.68 -1.2 418,709 + Atu3376 0.74 +1.0 418,756 + Atu3376 0.80 +0.2 418,842 + Atu3376 0.90 +0.5 418,850 - +1.6 418,886 - -0.3 418,905 - +0.1 419,034 + +0.5 419,034 + -1.8 419,042 - -0.6 419,133 + Atu3377 0.12 -2.6 419,135 - Atu3377 0.12 +0.2 419,243 - Atu3377 0.19 -0.4 419,323 + Atu3377 0.24 -1.5 419,328 + Atu3377 0.24 -3.0 419,336 - Atu3377 0.24 +0.9 419,341 - Atu3377 0.25 -0.4 419,343 + Atu3377 0.25 +0.2 419,349 + Atu3377 0.25 -0.1 419,349 + Atu3377 0.25 -0.9 419,349 + Atu3377 0.25 -0.9 419,351 - Atu3377 0.25 -0.0 419,405 - Atu3377 0.29 +0.1 419,431 - Atu3377 0.30 +1.4 419,455 - Atu3377 0.32 +1.4 419,455 - Atu3377 0.32 +0.1 419,476 - Atu3377 0.33 -2.5 419,497 + Atu3377 0.34 +2.1 419,602 - Atu3377 0.41 -0.6 419,698 - Atu3377 0.47 -1.2 419,766 + Atu3377 0.51 +0.6 419,774 - Atu3377 0.51 +2.0 419,799 - Atu3377 0.53 -1.1 419,847 + Atu3377 0.56 -0.5 419,860 + Atu3377 0.57 -0.5 419,906 + Atu3377 0.59 -1.9 419,918 + Atu3377 0.60 -0.6 419,938 + Atu3377 0.61 +1.0 419,988 + Atu3377 0.64 -1.4 419,988 - Atu3377 0.64 +0.2 420,068 + Atu3377 0.69 -0.7 420,290 + Atu3377 0.83 -1.4 420,305 - Atu3377 0.84 +1.9 420,340 + Atu3377 0.86 -0.9 420,394 + Atu3377 0.89 -0.1 420,494 + -1.0 420,547 + -1.3 420,577 + -1.7 420,590 + -3.1 420,598 - -0.7 420,676 - Atu3378 0.13 +0.0 420,746 + Atu3378 0.22 +0.2 420,754 - Atu3378 0.23 -0.2 420,762 + Atu3378 0.24 +0.6 420,832 - Atu3378 0.32 +1.1 420,923 + Atu3378 0.43 -0.1 420,923 + Atu3378 0.43 +0.2 420,926 - Atu3378 0.44 -0.1 420,931 - Atu3378 0.44 +0.6 421,019 + Atu3378 0.55 -0.6 421,231 - Atu3378 0.81 +0.6 421,281 - Atu3378 0.87 +0.6 421,300 + Atu3378 0.89 -1.2 421,300 + Atu3378 0.89 +0.6 421,305 + -0.7 421,308 - -0.7 421,308 - +1.5 421,318 - -0.4 421,329 + -1.0 421,377 - -1.0 421,413 + +0.8 421,421 - -0.6 421,421 - -0.1 421,483 - Atu3379 0.11 -0.7 421,488 + Atu3379 0.11 +1.4 421,541 + Atu3379 0.17 +1.2 421,541 + Atu3379 0.17 -2.0
Or see this region's nucleotide sequence