Experiment: Chloramphenicol 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0501 and Atu0502 are separated by 38 nucleotides Atu0502 and Atu0503 are separated by 184 nucleotides
Atu0501: Atu0501 - acyl-CoA dehydrogenase, at 489,997 to 491,793
Atu0501
Atu0502: Atu0502 - acetyl-CoA acetyltransferase, at 491,832 to 493,040
Atu0502
Atu0503: Atu0503 - enoyl-CoA hydratase, at 493,225 to 495,441
Atu0503
Position (kb) 491
492
493
494 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 490.856 kb on - strand, within Atu0501 at 490.889 kb on + strand, within Atu0501 at 490.897 kb on - strand, within Atu0501 at 490.955 kb on + strand, within Atu0501 at 490.960 kb on + strand, within Atu0501 at 490.968 kb on - strand, within Atu0501 at 490.968 kb on - strand, within Atu0501 at 491.050 kb on - strand, within Atu0501 at 491.220 kb on + strand, within Atu0501 at 491.279 kb on + strand, within Atu0501 at 491.330 kb on + strand, within Atu0501 at 491.405 kb on - strand, within Atu0501 at 491.453 kb on - strand, within Atu0501 at 491.582 kb on + strand, within Atu0501 at 491.592 kb on + strand, within Atu0501 at 491.592 kb on + strand, within Atu0501 at 491.592 kb on + strand, within Atu0501 at 491.694 kb on + strand at 491.804 kb on - strand at 491.896 kb on - strand at 492.106 kb on - strand, within Atu0502 at 492.143 kb on + strand, within Atu0502 at 492.151 kb on - strand, within Atu0502 at 492.332 kb on + strand, within Atu0502 at 492.413 kb on + strand, within Atu0502 at 492.414 kb on + strand, within Atu0502 at 492.496 kb on - strand, within Atu0502 at 492.508 kb on - strand, within Atu0502 at 492.637 kb on + strand, within Atu0502 at 492.637 kb on + strand, within Atu0502 at 492.637 kb on + strand, within Atu0502 at 492.645 kb on - strand, within Atu0502 at 492.645 kb on - strand, within Atu0502 at 492.683 kb on + strand, within Atu0502 at 492.683 kb on + strand, within Atu0502 at 492.683 kb on + strand, within Atu0502 at 492.942 kb on + strand at 492.966 kb on + strand at 493.000 kb on - strand at 493.064 kb on + strand at 493.069 kb on + strand at 493.069 kb on + strand at 493.072 kb on - strand at 493.103 kb on + strand at 493.124 kb on - strand at 493.170 kb on + strand at 493.239 kb on - strand at 493.268 kb on + strand at 493.276 kb on - strand at 493.389 kb on + strand at 493.392 kb on - strand at 493.517 kb on - strand, within Atu0503 at 493.549 kb on + strand, within Atu0503 at 493.557 kb on - strand, within Atu0503 at 493.615 kb on - strand, within Atu0503 at 493.758 kb on - strand, within Atu0503 at 493.769 kb on - strand, within Atu0503 at 493.785 kb on - strand, within Atu0503 at 493.800 kb on + strand, within Atu0503 at 493.850 kb on + strand, within Atu0503 at 493.882 kb on - strand, within Atu0503 at 493.892 kb on + strand, within Atu0503 at 493.975 kb on + strand, within Atu0503
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 12.5 ug/mL remove 490,856 - Atu0501 0.48 -0.1 490,889 + Atu0501 0.50 +1.5 490,897 - Atu0501 0.50 +0.7 490,955 + Atu0501 0.53 +1.3 490,960 + Atu0501 0.54 +4.5 490,968 - Atu0501 0.54 +1.6 490,968 - Atu0501 0.54 +1.5 491,050 - Atu0501 0.59 -0.3 491,220 + Atu0501 0.68 -0.5 491,279 + Atu0501 0.71 +1.4 491,330 + Atu0501 0.74 +0.5 491,405 - Atu0501 0.78 +2.6 491,453 - Atu0501 0.81 -0.5 491,582 + Atu0501 0.88 +0.6 491,592 + Atu0501 0.89 -1.7 491,592 + Atu0501 0.89 +0.8 491,592 + Atu0501 0.89 +0.5 491,694 + +1.5 491,804 - -2.4 491,896 - -0.7 492,106 - Atu0502 0.23 +4.7 492,143 + Atu0502 0.26 -0.5 492,151 - Atu0502 0.26 +3.0 492,332 + Atu0502 0.41 -2.9 492,413 + Atu0502 0.48 +0.3 492,414 + Atu0502 0.48 +1.4 492,496 - Atu0502 0.55 -1.8 492,508 - Atu0502 0.56 -2.4 492,637 + Atu0502 0.67 +0.5 492,637 + Atu0502 0.67 -0.1 492,637 + Atu0502 0.67 -0.1 492,645 - Atu0502 0.67 +1.0 492,645 - Atu0502 0.67 -0.5 492,683 + Atu0502 0.70 +0.7 492,683 + Atu0502 0.70 -0.4 492,683 + Atu0502 0.70 +1.1 492,942 + -0.1 492,966 + +0.3 493,000 - +0.0 493,064 + -0.9 493,069 + -0.8 493,069 + +0.4 493,072 - +1.1 493,103 + -0.5 493,124 - +1.2 493,170 + -0.5 493,239 - +1.4 493,268 + -2.1 493,276 - -0.1 493,389 + +0.9 493,392 - +0.3 493,517 - Atu0503 0.13 -0.7 493,549 + Atu0503 0.15 -0.2 493,557 - Atu0503 0.15 -0.1 493,615 - Atu0503 0.18 -0.8 493,758 - Atu0503 0.24 -0.4 493,769 - Atu0503 0.25 +4.1 493,785 - Atu0503 0.25 +1.5 493,800 + Atu0503 0.26 -0.5 493,850 + Atu0503 0.28 +0.3 493,882 - Atu0503 0.30 -0.4 493,892 + Atu0503 0.30 +0.2 493,975 + Atu0503 0.34 +0.3
Or see this region's nucleotide sequence