Experiment: Chloramphenicol 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0145 and Atu0146 overlap by 4 nucleotides Atu0146 and Atu0147 are separated by 38 nucleotides Atu0147 and Atu0148 are separated by 83 nucleotides
Atu0145: Atu0145 - glutamate synthase large subunit, at 148,937 to 154,285
Atu0145
Atu0146: Atu0146 - sulfite reductase (NADPH) flavoprotein alpha-component, at 154,282 to 156,051
Atu0146
Atu0147: Atu0147 - hypothetical protein, at 156,090 to 156,596
Atu0147
Atu0148: Atu0148 - Atu0148-1 mutant of a conserved hypothetical protein, at 156,680 to 157,276
Atu0148
Position (kb) 154
155
156
157 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 153.302 kb on + strand, within Atu0145 at 153.318 kb on + strand, within Atu0145 at 153.401 kb on + strand, within Atu0145 at 153.428 kb on + strand, within Atu0145 at 153.512 kb on + strand, within Atu0145 at 153.559 kb on + strand, within Atu0145 at 153.559 kb on + strand, within Atu0145 at 153.624 kb on - strand, within Atu0145 at 153.754 kb on - strand at 153.776 kb on - strand at 153.805 kb on + strand at 153.830 kb on + strand at 153.888 kb on - strand at 153.888 kb on - strand at 153.890 kb on - strand at 153.898 kb on - strand at 153.900 kb on - strand at 153.907 kb on + strand at 153.910 kb on - strand at 153.910 kb on - strand at 153.944 kb on - strand at 153.944 kb on - strand at 153.953 kb on - strand at 154.012 kb on - strand at 154.026 kb on - strand at 154.107 kb on - strand at 154.109 kb on + strand at 154.109 kb on + strand at 154.152 kb on + strand at 154.174 kb on - strand at 154.223 kb on - strand at 154.241 kb on + strand at 154.245 kb on - strand at 154.273 kb on - strand at 154.275 kb on + strand at 154.278 kb on - strand at 154.325 kb on - strand at 154.557 kb on - strand, within Atu0146 at 154.654 kb on + strand, within Atu0146 at 154.757 kb on + strand, within Atu0146 at 154.840 kb on + strand, within Atu0146 at 154.848 kb on - strand, within Atu0146 at 154.953 kb on + strand, within Atu0146 at 154.953 kb on + strand, within Atu0146 at 154.994 kb on + strand, within Atu0146 at 155.012 kb on - strand, within Atu0146 at 155.037 kb on + strand, within Atu0146 at 155.078 kb on + strand, within Atu0146 at 155.124 kb on - strand, within Atu0146 at 155.275 kb on - strand, within Atu0146 at 155.276 kb on - strand, within Atu0146 at 155.335 kb on - strand, within Atu0146 at 155.345 kb on - strand, within Atu0146 at 155.388 kb on + strand, within Atu0146 at 155.408 kb on + strand, within Atu0146 at 155.423 kb on + strand, within Atu0146 at 155.478 kb on + strand, within Atu0146 at 155.535 kb on + strand, within Atu0146 at 155.542 kb on + strand, within Atu0146 at 155.548 kb on - strand, within Atu0146 at 155.647 kb on - strand, within Atu0146 at 155.671 kb on + strand, within Atu0146 at 155.856 kb on - strand, within Atu0146 at 155.876 kb on + strand at 155.876 kb on + strand at 155.913 kb on + strand at 155.952 kb on - strand at 156.053 kb on - strand at 156.081 kb on + strand at 156.095 kb on + strand at 156.184 kb on + strand, within Atu0147 at 156.240 kb on + strand, within Atu0147 at 156.272 kb on + strand, within Atu0147 at 156.435 kb on - strand, within Atu0147 at 156.530 kb on + strand, within Atu0147 at 156.665 kb on + strand at 156.759 kb on - strand, within Atu0148 at 156.841 kb on - strand, within Atu0148
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 12.5 ug/mL remove 153,302 + Atu0145 0.82 +0.2 153,318 + Atu0145 0.82 +2.3 153,401 + Atu0145 0.83 -2.1 153,428 + Atu0145 0.84 -0.5 153,512 + Atu0145 0.86 -0.9 153,559 + Atu0145 0.86 +0.1 153,559 + Atu0145 0.86 -0.4 153,624 - Atu0145 0.88 -1.9 153,754 - -1.0 153,776 - -1.3 153,805 + -0.8 153,830 + -0.1 153,888 - -0.8 153,888 - -2.7 153,890 - +1.3 153,898 - +2.0 153,900 - -0.3 153,907 + +0.3 153,910 - +4.0 153,910 - -1.7 153,944 - +0.5 153,944 - -1.7 153,953 - -0.5 154,012 - -3.2 154,026 - +0.1 154,107 - -0.0 154,109 + +1.6 154,109 + +0.6 154,152 + -0.4 154,174 - -0.1 154,223 - +0.3 154,241 + -0.1 154,245 - +1.5 154,273 - -1.4 154,275 + -2.1 154,278 - -0.4 154,325 - -0.3 154,557 - Atu0146 0.16 -1.4 154,654 + Atu0146 0.21 -0.2 154,757 + Atu0146 0.27 +2.6 154,840 + Atu0146 0.32 +0.7 154,848 - Atu0146 0.32 +0.2 154,953 + Atu0146 0.38 +0.5 154,953 + Atu0146 0.38 +1.4 154,994 + Atu0146 0.40 -0.4 155,012 - Atu0146 0.41 +0.4 155,037 + Atu0146 0.43 -0.3 155,078 + Atu0146 0.45 +0.7 155,124 - Atu0146 0.48 -0.7 155,275 - Atu0146 0.56 -0.5 155,276 - Atu0146 0.56 +0.2 155,335 - Atu0146 0.59 -2.8 155,345 - Atu0146 0.60 -2.5 155,388 + Atu0146 0.62 -0.8 155,408 + Atu0146 0.64 -1.8 155,423 + Atu0146 0.64 +1.0 155,478 + Atu0146 0.68 +0.7 155,535 + Atu0146 0.71 -0.9 155,542 + Atu0146 0.71 +0.1 155,548 - Atu0146 0.72 -0.9 155,647 - Atu0146 0.77 +0.7 155,671 + Atu0146 0.78 +0.9 155,856 - Atu0146 0.89 -0.2 155,876 + +0.6 155,876 + -1.1 155,913 + +0.9 155,952 - -1.9 156,053 - +1.7 156,081 + +0.6 156,095 + +0.5 156,184 + Atu0147 0.19 -0.0 156,240 + Atu0147 0.30 +0.2 156,272 + Atu0147 0.36 -2.2 156,435 - Atu0147 0.68 +1.0 156,530 + Atu0147 0.87 -1.1 156,665 + -3.5 156,759 - Atu0148 0.13 -0.1 156,841 - Atu0148 0.27 -1.1
Or see this region's nucleotide sequence