Experiment: Chloramphenicol 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5497 and Atu5498 are separated by 127 nucleotides Atu5498 and Atu5499 overlap by 4 nucleotides Atu5499 and Atu5500 overlap by 4 nucleotides
Atu5497: Atu5497 - hypothetical protein, at 487,132 to 488,121
Atu5497
Atu5498: Atu5498 - oxidoreductase with iron-sulfur subunit, at 488,249 to 488,782
Atu5498
Atu5499: Atu5499 - oxidoreductase, at 488,779 to 489,768
Atu5499
Atu5500: Atu5500 - dehydrogenase, at 489,765 to 492,068
Atu5500
Position (kb) 488
489 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 487.263 kb on - strand, within Atu5497 at 487.283 kb on + strand, within Atu5497 at 487.283 kb on + strand, within Atu5497 at 487.349 kb on + strand, within Atu5497 at 487.353 kb on + strand, within Atu5497 at 487.353 kb on + strand, within Atu5497 at 487.361 kb on - strand, within Atu5497 at 487.523 kb on + strand, within Atu5497 at 487.531 kb on - strand, within Atu5497 at 487.560 kb on - strand, within Atu5497 at 487.571 kb on - strand, within Atu5497 at 487.601 kb on + strand, within Atu5497 at 487.601 kb on + strand, within Atu5497 at 487.625 kb on + strand, within Atu5497 at 487.654 kb on - strand, within Atu5497 at 487.708 kb on - strand, within Atu5497 at 487.812 kb on - strand, within Atu5497 at 487.938 kb on - strand, within Atu5497 at 487.940 kb on + strand, within Atu5497 at 487.948 kb on - strand, within Atu5497 at 487.959 kb on - strand, within Atu5497 at 488.040 kb on + strand at 488.040 kb on + strand at 488.040 kb on + strand at 488.048 kb on - strand at 488.048 kb on - strand at 488.048 kb on - strand at 488.055 kb on + strand at 488.095 kb on + strand at 488.140 kb on - strand at 488.162 kb on - strand at 488.212 kb on - strand at 488.244 kb on + strand at 488.252 kb on - strand at 488.308 kb on + strand, within Atu5498 at 488.316 kb on - strand, within Atu5498 at 488.356 kb on - strand, within Atu5498 at 488.368 kb on + strand, within Atu5498 at 488.395 kb on - strand, within Atu5498 at 488.430 kb on - strand, within Atu5498 at 488.440 kb on - strand, within Atu5498 at 488.462 kb on + strand, within Atu5498 at 488.470 kb on - strand, within Atu5498 at 488.470 kb on - strand, within Atu5498 at 488.496 kb on - strand, within Atu5498 at 488.496 kb on - strand, within Atu5498 at 488.557 kb on + strand, within Atu5498 at 488.557 kb on + strand, within Atu5498 at 488.624 kb on - strand, within Atu5498 at 488.714 kb on + strand, within Atu5498 at 488.717 kb on - strand, within Atu5498 at 488.733 kb on + strand at 488.748 kb on + strand at 488.777 kb on - strand at 488.862 kb on + strand at 488.900 kb on + strand, within Atu5499 at 488.908 kb on - strand, within Atu5499 at 488.935 kb on + strand, within Atu5499 at 488.943 kb on - strand, within Atu5499 at 488.943 kb on - strand, within Atu5499 at 488.977 kb on - strand, within Atu5499 at 488.984 kb on + strand, within Atu5499 at 488.989 kb on + strand, within Atu5499 at 488.997 kb on - strand, within Atu5499 at 489.002 kb on - strand, within Atu5499 at 489.002 kb on - strand, within Atu5499 at 489.030 kb on + strand, within Atu5499 at 489.056 kb on - strand, within Atu5499 at 489.060 kb on + strand, within Atu5499 at 489.243 kb on - strand, within Atu5499 at 489.260 kb on + strand, within Atu5499 at 489.291 kb on + strand, within Atu5499 at 489.481 kb on + strand, within Atu5499 at 489.481 kb on + strand, within Atu5499 at 489.489 kb on - strand, within Atu5499 at 489.525 kb on + strand, within Atu5499 at 489.527 kb on - strand, within Atu5499 at 489.540 kb on + strand, within Atu5499 at 489.736 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 12.5 ug/mL remove 487,263 - Atu5497 0.13 -0.0 487,283 + Atu5497 0.15 -0.4 487,283 + Atu5497 0.15 +1.1 487,349 + Atu5497 0.22 -2.3 487,353 + Atu5497 0.22 -1.0 487,353 + Atu5497 0.22 +0.2 487,361 - Atu5497 0.23 -0.4 487,523 + Atu5497 0.39 -0.7 487,531 - Atu5497 0.40 -0.9 487,560 - Atu5497 0.43 +0.9 487,571 - Atu5497 0.44 -1.3 487,601 + Atu5497 0.47 -0.7 487,601 + Atu5497 0.47 +0.9 487,625 + Atu5497 0.50 +0.2 487,654 - Atu5497 0.53 +0.6 487,708 - Atu5497 0.58 +0.1 487,812 - Atu5497 0.69 -2.5 487,938 - Atu5497 0.81 +0.6 487,940 + Atu5497 0.82 -0.2 487,948 - Atu5497 0.82 +0.4 487,959 - Atu5497 0.84 +0.2 488,040 + +0.8 488,040 + +0.6 488,040 + +0.0 488,048 - -0.9 488,048 - -0.2 488,048 - -0.2 488,055 + -0.2 488,095 + +0.5 488,140 - -3.0 488,162 - +0.2 488,212 - -0.9 488,244 + +1.6 488,252 - +0.3 488,308 + Atu5498 0.11 +2.4 488,316 - Atu5498 0.13 +1.3 488,356 - Atu5498 0.20 -0.7 488,368 + Atu5498 0.22 +0.0 488,395 - Atu5498 0.27 +1.6 488,430 - Atu5498 0.34 -0.8 488,440 - Atu5498 0.36 +0.1 488,462 + Atu5498 0.40 +0.0 488,470 - Atu5498 0.41 +1.5 488,470 - Atu5498 0.41 -0.2 488,496 - Atu5498 0.46 +1.6 488,496 - Atu5498 0.46 +0.8 488,557 + Atu5498 0.58 -1.0 488,557 + Atu5498 0.58 +0.0 488,624 - Atu5498 0.70 -0.4 488,714 + Atu5498 0.87 -0.7 488,717 - Atu5498 0.88 +1.2 488,733 + -0.8 488,748 + +2.5 488,777 - +0.8 488,862 + -0.4 488,900 + Atu5499 0.12 -1.2 488,908 - Atu5499 0.13 -0.9 488,935 + Atu5499 0.16 +1.3 488,943 - Atu5499 0.17 -0.3 488,943 - Atu5499 0.17 -1.2 488,977 - Atu5499 0.20 +0.4 488,984 + Atu5499 0.21 +1.7 488,989 + Atu5499 0.21 -3.3 488,997 - Atu5499 0.22 -2.0 489,002 - Atu5499 0.23 -0.4 489,002 - Atu5499 0.23 -0.7 489,030 + Atu5499 0.25 -1.1 489,056 - Atu5499 0.28 -1.8 489,060 + Atu5499 0.28 -0.3 489,243 - Atu5499 0.47 +1.8 489,260 + Atu5499 0.49 -1.2 489,291 + Atu5499 0.52 -0.4 489,481 + Atu5499 0.71 +2.2 489,481 + Atu5499 0.71 -1.7 489,489 - Atu5499 0.72 -1.5 489,525 + Atu5499 0.75 -0.1 489,527 - Atu5499 0.76 -0.3 489,540 + Atu5499 0.77 +0.3 489,736 + +0.6
Or see this region's nucleotide sequence