Experiment: Chloramphenicol 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5068 and Atu5069 overlap by 8 nucleotides Atu5069 and Atu5070 are separated by 14 nucleotides
Atu5068: Atu5068 - ABC transporter nucleotide binding/ATPase (oligopeptide), at 66,348 to 67,967
Atu5068
Atu5069: Atu5069 - ABC transporter membrane spanning protein (oligopeptide), at 67,960 to 68,907
Atu5069
Atu5070: Atu5070 - ABC transporter membrane spanning protein (oligopeptide), at 68,922 to 69,836
Atu5070
Position (kb)
67
68
69 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 66.962 kb on - strand, within Atu5068 at 67.128 kb on + strand, within Atu5068 at 67.158 kb on + strand, within Atu5068 at 67.165 kb on + strand, within Atu5068 at 67.167 kb on - strand, within Atu5068 at 67.167 kb on - strand, within Atu5068 at 67.173 kb on + strand, within Atu5068 at 67.221 kb on - strand, within Atu5068 at 67.330 kb on - strand, within Atu5068 at 67.369 kb on + strand, within Atu5068 at 67.369 kb on + strand, within Atu5068 at 67.369 kb on + strand, within Atu5068 at 67.369 kb on + strand, within Atu5068 at 67.369 kb on + strand, within Atu5068 at 67.377 kb on - strand, within Atu5068 at 67.377 kb on - strand, within Atu5068 at 67.396 kb on - strand, within Atu5068 at 67.410 kb on - strand, within Atu5068 at 67.528 kb on + strand, within Atu5068 at 67.560 kb on - strand, within Atu5068 at 67.570 kb on + strand, within Atu5068 at 67.570 kb on + strand, within Atu5068 at 67.578 kb on - strand, within Atu5068 at 67.583 kb on - strand, within Atu5068 at 67.633 kb on - strand, within Atu5068 at 67.648 kb on + strand, within Atu5068 at 67.656 kb on - strand, within Atu5068 at 67.695 kb on + strand, within Atu5068 at 67.703 kb on - strand, within Atu5068 at 67.749 kb on + strand, within Atu5068 at 67.762 kb on - strand, within Atu5068 at 67.820 kb on - strand at 67.826 kb on + strand at 67.834 kb on - strand at 67.878 kb on + strand at 67.897 kb on + strand at 67.905 kb on - strand at 68.004 kb on - strand at 68.004 kb on - strand at 68.006 kb on - strand at 68.009 kb on - strand at 68.009 kb on - strand at 68.009 kb on - strand at 68.009 kb on - strand at 68.009 kb on - strand at 68.154 kb on - strand, within Atu5069 at 68.187 kb on - strand, within Atu5069 at 68.224 kb on + strand, within Atu5069 at 68.242 kb on + strand, within Atu5069 at 68.364 kb on - strand, within Atu5069 at 68.404 kb on + strand, within Atu5069 at 68.539 kb on - strand, within Atu5069 at 68.539 kb on - strand, within Atu5069 at 68.551 kb on - strand, within Atu5069 at 68.571 kb on + strand, within Atu5069 at 68.645 kb on + strand, within Atu5069 at 68.736 kb on + strand, within Atu5069 at 68.736 kb on + strand, within Atu5069 at 68.736 kb on + strand, within Atu5069 at 68.736 kb on + strand, within Atu5069 at 68.739 kb on - strand, within Atu5069 at 68.744 kb on - strand, within Atu5069 at 68.744 kb on - strand, within Atu5069 at 68.744 kb on - strand, within Atu5069 at 68.744 kb on - strand, within Atu5069 at 68.807 kb on + strand, within Atu5069 at 68.885 kb on + strand at 68.885 kb on + strand at 68.893 kb on - strand at 68.950 kb on + strand at 68.988 kb on + strand at 69.108 kb on + strand, within Atu5070 at 69.136 kb on - strand, within Atu5070 at 69.220 kb on + strand, within Atu5070 at 69.220 kb on + strand, within Atu5070 at 69.220 kb on + strand, within Atu5070 at 69.220 kb on + strand, within Atu5070 at 69.228 kb on - strand, within Atu5070 at 69.228 kb on - strand, within Atu5070 at 69.228 kb on - strand, within Atu5070 at 69.228 kb on - strand, within Atu5070 at 69.238 kb on - strand, within Atu5070 at 69.259 kb on - strand, within Atu5070 at 69.281 kb on + strand, within Atu5070 at 69.510 kb on - strand, within Atu5070 at 69.546 kb on + strand, within Atu5070 at 69.546 kb on + strand, within Atu5070 at 69.547 kb on - strand, within Atu5070 at 69.573 kb on + strand, within Atu5070 at 69.574 kb on - strand, within Atu5070 at 69.618 kb on + strand, within Atu5070 at 69.666 kb on + strand, within Atu5070 at 69.669 kb on - strand, within Atu5070 at 69.673 kb on - strand, within Atu5070 at 69.682 kb on + strand, within Atu5070 at 69.794 kb on + strand at 69.842 kb on + strand at 69.855 kb on + strand at 69.887 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 12.5 ug/mL remove 66,962 - Atu5068 0.38 -1.6 67,128 + Atu5068 0.48 +0.0 67,158 + Atu5068 0.50 +0.6 67,165 + Atu5068 0.50 -0.1 67,167 - Atu5068 0.51 +0.1 67,167 - Atu5068 0.51 -0.0 67,173 + Atu5068 0.51 -0.6 67,221 - Atu5068 0.54 -0.5 67,330 - Atu5068 0.61 -1.1 67,369 + Atu5068 0.63 +1.5 67,369 + Atu5068 0.63 -0.9 67,369 + Atu5068 0.63 -1.0 67,369 + Atu5068 0.63 +0.8 67,369 + Atu5068 0.63 -1.2 67,377 - Atu5068 0.64 -2.4 67,377 - Atu5068 0.64 -0.5 67,396 - Atu5068 0.65 +2.4 67,410 - Atu5068 0.66 -2.0 67,528 + Atu5068 0.73 -0.6 67,560 - Atu5068 0.75 +0.2 67,570 + Atu5068 0.75 -0.1 67,570 + Atu5068 0.75 +1.3 67,578 - Atu5068 0.76 +0.0 67,583 - Atu5068 0.76 +1.0 67,633 - Atu5068 0.79 -0.5 67,648 + Atu5068 0.80 -0.8 67,656 - Atu5068 0.81 -1.4 67,695 + Atu5068 0.83 -0.1 67,703 - Atu5068 0.84 -4.6 67,749 + Atu5068 0.86 +0.2 67,762 - Atu5068 0.87 -0.5 67,820 - -0.0 67,826 + -0.5 67,834 - -1.7 67,878 + +0.1 67,897 + -0.9 67,905 - -1.1 68,004 - -0.5 68,004 - -0.3 68,006 - -1.8 68,009 - -0.3 68,009 - -0.1 68,009 - -2.1 68,009 - -1.7 68,009 - -1.2 68,154 - Atu5069 0.20 -0.4 68,187 - Atu5069 0.24 +0.8 68,224 + Atu5069 0.28 +1.6 68,242 + Atu5069 0.30 -2.2 68,364 - Atu5069 0.43 +0.3 68,404 + Atu5069 0.47 -1.0 68,539 - Atu5069 0.61 -1.3 68,539 - Atu5069 0.61 +0.4 68,551 - Atu5069 0.62 -2.7 68,571 + Atu5069 0.64 +0.6 68,645 + Atu5069 0.72 -0.1 68,736 + Atu5069 0.82 -0.2 68,736 + Atu5069 0.82 -1.4 68,736 + Atu5069 0.82 -0.0 68,736 + Atu5069 0.82 -0.1 68,739 - Atu5069 0.82 -0.4 68,744 - Atu5069 0.83 +0.0 68,744 - Atu5069 0.83 -1.7 68,744 - Atu5069 0.83 +0.8 68,744 - Atu5069 0.83 -1.1 68,807 + Atu5069 0.89 +0.2 68,885 + +0.2 68,885 + -1.0 68,893 - +0.3 68,950 + -0.1 68,988 + -2.2 69,108 + Atu5070 0.20 +0.2 69,136 - Atu5070 0.23 -0.1 69,220 + Atu5070 0.33 +0.8 69,220 + Atu5070 0.33 +1.2 69,220 + Atu5070 0.33 +0.4 69,220 + Atu5070 0.33 -0.2 69,228 - Atu5070 0.33 +0.6 69,228 - Atu5070 0.33 +0.3 69,228 - Atu5070 0.33 +0.8 69,228 - Atu5070 0.33 +0.3 69,238 - Atu5070 0.35 -2.1 69,259 - Atu5070 0.37 +0.5 69,281 + Atu5070 0.39 +0.5 69,510 - Atu5070 0.64 -0.4 69,546 + Atu5070 0.68 -0.8 69,546 + Atu5070 0.68 -0.8 69,547 - Atu5070 0.68 +0.4 69,573 + Atu5070 0.71 -0.2 69,574 - Atu5070 0.71 -0.3 69,618 + Atu5070 0.76 -0.5 69,666 + Atu5070 0.81 -0.2 69,669 - Atu5070 0.82 -1.0 69,673 - Atu5070 0.82 -0.7 69,682 + Atu5070 0.83 +0.3 69,794 + -1.4 69,842 + -0.4 69,855 + -0.0 69,887 - +0.8
Or see this region's nucleotide sequence