Experiment: Chloramphenicol 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4655 and Atu4656 are separated by 19 nucleotides Atu4656 and Atu4657 overlap by 4 nucleotides Atu4657 and Atu4658 are separated by 9 nucleotides
Atu4655: Atu4655 - ROK family transcriptional regulator, at 1,809,583 to 1,810,809
Atu4655
Atu4656: Atu4656 - hypothetical protein, at 1,810,829 to 1,813,231
Atu4656
Atu4657: Atu4657 - hypothetical protein, at 1,813,228 to 1,814,139
Atu4657
Atu4658: Atu4658 - hypothetical protein, at 1,814,149 to 1,814,682
Atu4658
Position (kb)
1810
1811
1812
1813
1814 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1809.834 kb on - strand, within Atu4655 at 1809.937 kb on - strand, within Atu4655 at 1809.972 kb on + strand, within Atu4655 at 1809.973 kb on + strand, within Atu4655 at 1809.973 kb on + strand, within Atu4655 at 1810.037 kb on - strand, within Atu4655 at 1810.143 kb on + strand, within Atu4655 at 1810.211 kb on + strand, within Atu4655 at 1810.219 kb on - strand, within Atu4655 at 1810.276 kb on + strand, within Atu4655 at 1810.350 kb on + strand, within Atu4655 at 1810.358 kb on - strand, within Atu4655 at 1810.364 kb on - strand, within Atu4655 at 1810.393 kb on + strand, within Atu4655 at 1810.401 kb on - strand, within Atu4655 at 1810.401 kb on - strand, within Atu4655 at 1810.442 kb on - strand, within Atu4655 at 1810.465 kb on + strand, within Atu4655 at 1810.476 kb on + strand, within Atu4655 at 1810.476 kb on + strand, within Atu4655 at 1810.484 kb on - strand, within Atu4655 at 1810.484 kb on - strand, within Atu4655 at 1810.484 kb on - strand, within Atu4655 at 1810.585 kb on - strand, within Atu4655 at 1810.599 kb on + strand, within Atu4655 at 1810.607 kb on - strand, within Atu4655 at 1810.649 kb on + strand, within Atu4655 at 1810.988 kb on + strand at 1810.996 kb on - strand at 1810.996 kb on - strand at 1811.052 kb on + strand at 1811.059 kb on - strand at 1811.098 kb on + strand, within Atu4656 at 1811.106 kb on - strand, within Atu4656 at 1811.213 kb on + strand, within Atu4656 at 1811.226 kb on - strand, within Atu4656 at 1811.228 kb on + strand, within Atu4656 at 1811.322 kb on - strand, within Atu4656 at 1811.406 kb on - strand, within Atu4656 at 1811.509 kb on + strand, within Atu4656 at 1811.517 kb on - strand, within Atu4656 at 1811.517 kb on - strand, within Atu4656 at 1811.534 kb on + strand, within Atu4656 at 1811.636 kb on - strand, within Atu4656 at 1811.761 kb on + strand, within Atu4656 at 1811.810 kb on + strand, within Atu4656 at 1811.834 kb on - strand, within Atu4656 at 1811.844 kb on + strand, within Atu4656 at 1811.844 kb on + strand, within Atu4656 at 1811.877 kb on - strand, within Atu4656 at 1811.914 kb on + strand, within Atu4656 at 1811.956 kb on + strand, within Atu4656 at 1811.980 kb on - strand, within Atu4656 at 1812.009 kb on - strand, within Atu4656 at 1812.159 kb on - strand, within Atu4656 at 1812.244 kb on - strand, within Atu4656 at 1812.326 kb on + strand, within Atu4656 at 1812.342 kb on + strand, within Atu4656 at 1812.350 kb on - strand, within Atu4656 at 1812.355 kb on + strand, within Atu4656 at 1812.363 kb on - strand, within Atu4656 at 1812.445 kb on + strand, within Atu4656 at 1812.458 kb on - strand, within Atu4656 at 1812.593 kb on - strand, within Atu4656 at 1812.595 kb on + strand, within Atu4656 at 1812.603 kb on - strand, within Atu4656 at 1812.864 kb on - strand, within Atu4656 at 1812.902 kb on + strand, within Atu4656 at 1812.912 kb on - strand, within Atu4656 at 1812.971 kb on + strand, within Atu4656 at 1812.972 kb on + strand, within Atu4656 at 1813.012 kb on - strand at 1813.012 kb on - strand at 1813.029 kb on + strand at 1813.044 kb on + strand at 1813.052 kb on + strand at 1813.143 kb on + strand at 1813.152 kb on + strand at 1813.187 kb on - strand at 1813.187 kb on - strand at 1813.233 kb on - strand at 1813.235 kb on - strand at 1813.294 kb on + strand at 1813.344 kb on + strand, within Atu4657 at 1813.358 kb on + strand, within Atu4657 at 1813.383 kb on - strand, within Atu4657 at 1813.413 kb on - strand, within Atu4657 at 1813.442 kb on + strand, within Atu4657 at 1813.442 kb on + strand, within Atu4657 at 1813.796 kb on - strand, within Atu4657 at 1813.806 kb on + strand, within Atu4657 at 1813.806 kb on + strand, within Atu4657 at 1813.806 kb on + strand, within Atu4657 at 1813.813 kb on + strand, within Atu4657 at 1813.825 kb on + strand, within Atu4657 at 1813.874 kb on + strand, within Atu4657 at 1813.891 kb on - strand, within Atu4657 at 1813.996 kb on - strand, within Atu4657 at 1814.025 kb on + strand, within Atu4657 at 1814.110 kb on + strand at 1814.186 kb on + strand at 1814.192 kb on + strand at 1814.200 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 12.5 ug/mL remove 1,809,834 - Atu4655 0.20 -3.1 1,809,937 - Atu4655 0.29 +0.9 1,809,972 + Atu4655 0.32 +0.4 1,809,973 + Atu4655 0.32 +0.6 1,809,973 + Atu4655 0.32 -0.6 1,810,037 - Atu4655 0.37 -1.2 1,810,143 + Atu4655 0.46 +0.1 1,810,211 + Atu4655 0.51 -0.0 1,810,219 - Atu4655 0.52 -0.2 1,810,276 + Atu4655 0.56 +0.6 1,810,350 + Atu4655 0.63 -0.2 1,810,358 - Atu4655 0.63 +1.3 1,810,364 - Atu4655 0.64 +1.5 1,810,393 + Atu4655 0.66 -0.2 1,810,401 - Atu4655 0.67 +1.5 1,810,401 - Atu4655 0.67 -0.1 1,810,442 - Atu4655 0.70 +0.7 1,810,465 + Atu4655 0.72 -2.0 1,810,476 + Atu4655 0.73 +0.6 1,810,476 + Atu4655 0.73 -2.7 1,810,484 - Atu4655 0.73 -0.3 1,810,484 - Atu4655 0.73 -0.0 1,810,484 - Atu4655 0.73 -0.2 1,810,585 - Atu4655 0.82 +1.8 1,810,599 + Atu4655 0.83 +0.2 1,810,607 - Atu4655 0.83 -0.3 1,810,649 + Atu4655 0.87 +0.1 1,810,988 + +0.2 1,810,996 - -0.9 1,810,996 - +1.0 1,811,052 + +2.6 1,811,059 - -1.5 1,811,098 + Atu4656 0.11 -0.4 1,811,106 - Atu4656 0.12 +1.0 1,811,213 + Atu4656 0.16 +1.3 1,811,226 - Atu4656 0.17 +0.9 1,811,228 + Atu4656 0.17 +0.5 1,811,322 - Atu4656 0.21 +0.9 1,811,406 - Atu4656 0.24 -0.0 1,811,509 + Atu4656 0.28 +0.4 1,811,517 - Atu4656 0.29 +1.0 1,811,517 - Atu4656 0.29 -1.4 1,811,534 + Atu4656 0.29 +0.3 1,811,636 - Atu4656 0.34 +1.8 1,811,761 + Atu4656 0.39 -1.0 1,811,810 + Atu4656 0.41 -1.5 1,811,834 - Atu4656 0.42 +0.2 1,811,844 + Atu4656 0.42 -1.8 1,811,844 + Atu4656 0.42 +0.8 1,811,877 - Atu4656 0.44 +1.3 1,811,914 + Atu4656 0.45 -0.8 1,811,956 + Atu4656 0.47 -0.8 1,811,980 - Atu4656 0.48 +0.9 1,812,009 - Atu4656 0.49 +1.8 1,812,159 - Atu4656 0.55 -1.8 1,812,244 - Atu4656 0.59 +1.2 1,812,326 + Atu4656 0.62 +0.8 1,812,342 + Atu4656 0.63 +2.5 1,812,350 - Atu4656 0.63 +1.8 1,812,355 + Atu4656 0.64 -1.0 1,812,363 - Atu4656 0.64 -0.4 1,812,445 + Atu4656 0.67 +0.9 1,812,458 - Atu4656 0.68 -1.2 1,812,593 - Atu4656 0.73 -0.4 1,812,595 + Atu4656 0.73 +2.3 1,812,603 - Atu4656 0.74 -0.1 1,812,864 - Atu4656 0.85 +0.2 1,812,902 + Atu4656 0.86 +0.2 1,812,912 - Atu4656 0.87 -1.2 1,812,971 + Atu4656 0.89 +1.2 1,812,972 + Atu4656 0.89 +4.2 1,813,012 - -1.0 1,813,012 - +1.4 1,813,029 + -1.2 1,813,044 + +1.5 1,813,052 + -1.0 1,813,143 + +0.2 1,813,152 + +1.2 1,813,187 - -0.2 1,813,187 - +1.8 1,813,233 - -0.6 1,813,235 - -0.2 1,813,294 + +1.8 1,813,344 + Atu4657 0.13 -0.2 1,813,358 + Atu4657 0.14 +0.6 1,813,383 - Atu4657 0.17 -2.0 1,813,413 - Atu4657 0.20 +0.2 1,813,442 + Atu4657 0.23 -0.4 1,813,442 + Atu4657 0.23 -0.4 1,813,796 - Atu4657 0.62 +0.8 1,813,806 + Atu4657 0.63 +0.8 1,813,806 + Atu4657 0.63 +0.4 1,813,806 + Atu4657 0.63 -0.0 1,813,813 + Atu4657 0.64 -0.7 1,813,825 + Atu4657 0.65 +0.4 1,813,874 + Atu4657 0.71 +0.0 1,813,891 - Atu4657 0.73 +0.2 1,813,996 - Atu4657 0.84 +0.3 1,814,025 + Atu4657 0.87 -1.4 1,814,110 + +3.8 1,814,186 + +1.7 1,814,192 + -1.8 1,814,200 - -0.3
Or see this region's nucleotide sequence