Experiment: Chloramphenicol 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4538 and Atu4539 are separated by 12 nucleotides Atu4539 and Atu4540 are separated by 2 nucleotides Atu4540 and Atu4541 overlap by 4 nucleotides Atu4541 and Atu4542 are separated by 3 nucleotides
Atu4538: Atu4538 - 3-carboxy-cis,cis-muconate cycloisomerase, at 1,684,759 to 1,685,820
Atu4538
Atu4539: Atu4539 - protocatechuate 3,4-dioxygenase alpha chain, at 1,685,833 to 1,686,453
Atu4539
Atu4540: Atu4540 - protocatechuate 3,4-dioxygenase subunit beta, at 1,686,456 to 1,687,196
Atu4540
Atu4541: Atu4541 - carboxymuconolactone decarboxylase, at 1,687,193 to 1,687,627
Atu4541
Atu4542: Atu4542 - beta-ketoadipate enol-lactone hydrolase, at 1,687,631 to 1,688,428
Atu4542
Position (kb)
1686
1687
1688 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1685.601 kb on + strand, within Atu4538 at 1685.687 kb on + strand, within Atu4538 at 1685.689 kb on - strand, within Atu4538 at 1685.727 kb on - strand at 1685.744 kb on + strand at 1685.752 kb on - strand at 1685.760 kb on - strand at 1685.781 kb on + strand at 1685.789 kb on - strand at 1685.893 kb on - strand at 1685.912 kb on + strand, within Atu4539 at 1685.958 kb on - strand, within Atu4539 at 1685.984 kb on + strand, within Atu4539 at 1686.012 kb on - strand, within Atu4539 at 1686.014 kb on - strand, within Atu4539 at 1686.055 kb on - strand, within Atu4539 at 1686.092 kb on - strand, within Atu4539 at 1686.238 kb on - strand, within Atu4539 at 1686.247 kb on + strand, within Atu4539 at 1686.268 kb on + strand, within Atu4539 at 1686.268 kb on + strand, within Atu4539 at 1686.268 kb on + strand, within Atu4539 at 1686.268 kb on + strand, within Atu4539 at 1686.276 kb on - strand, within Atu4539 at 1686.276 kb on - strand, within Atu4539 at 1686.276 kb on - strand, within Atu4539 at 1686.276 kb on - strand, within Atu4539 at 1686.276 kb on - strand, within Atu4539 at 1686.452 kb on - strand at 1686.485 kb on + strand at 1686.511 kb on + strand at 1686.543 kb on - strand, within Atu4540 at 1686.543 kb on - strand, within Atu4540 at 1686.558 kb on - strand, within Atu4540 at 1686.630 kb on - strand, within Atu4540 at 1687.003 kb on - strand, within Atu4540 at 1687.134 kb on - strand at 1687.311 kb on + strand, within Atu4541 at 1687.319 kb on + strand, within Atu4541 at 1687.327 kb on - strand, within Atu4541 at 1687.356 kb on + strand, within Atu4541 at 1687.377 kb on + strand, within Atu4541 at 1687.385 kb on - strand, within Atu4541 at 1687.559 kb on + strand, within Atu4541 at 1687.581 kb on + strand, within Atu4541 at 1687.581 kb on + strand, within Atu4541 at 1687.669 kb on + strand at 1687.687 kb on - strand at 1687.690 kb on + strand at 1687.690 kb on + strand at 1687.715 kb on + strand, within Atu4542 at 1687.780 kb on + strand, within Atu4542 at 1687.830 kb on + strand, within Atu4542 at 1687.848 kb on - strand, within Atu4542 at 1687.948 kb on + strand, within Atu4542 at 1687.956 kb on - strand, within Atu4542 at 1687.956 kb on - strand, within Atu4542 at 1687.956 kb on - strand, within Atu4542 at 1687.991 kb on - strand, within Atu4542 at 1688.039 kb on + strand, within Atu4542 at 1688.094 kb on + strand, within Atu4542
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 12.5 ug/mL remove 1,685,601 + Atu4538 0.79 -0.1 1,685,687 + Atu4538 0.87 -0.4 1,685,689 - Atu4538 0.88 -1.0 1,685,727 - -2.1 1,685,744 + -1.8 1,685,752 - -0.6 1,685,760 - +0.2 1,685,781 + -0.9 1,685,789 - +0.1 1,685,893 - -0.0 1,685,912 + Atu4539 0.13 -0.1 1,685,958 - Atu4539 0.20 +0.6 1,685,984 + Atu4539 0.24 -1.9 1,686,012 - Atu4539 0.29 -0.6 1,686,014 - Atu4539 0.29 -1.6 1,686,055 - Atu4539 0.36 -0.4 1,686,092 - Atu4539 0.42 +0.5 1,686,238 - Atu4539 0.65 -0.7 1,686,247 + Atu4539 0.67 +1.8 1,686,268 + Atu4539 0.70 +0.7 1,686,268 + Atu4539 0.70 -0.0 1,686,268 + Atu4539 0.70 +0.3 1,686,268 + Atu4539 0.70 -1.0 1,686,276 - Atu4539 0.71 -0.5 1,686,276 - Atu4539 0.71 -1.2 1,686,276 - Atu4539 0.71 +0.8 1,686,276 - Atu4539 0.71 +0.5 1,686,276 - Atu4539 0.71 -1.9 1,686,452 - +2.9 1,686,485 + -0.2 1,686,511 + -1.0 1,686,543 - Atu4540 0.12 -1.4 1,686,543 - Atu4540 0.12 -1.7 1,686,558 - Atu4540 0.14 +0.6 1,686,630 - Atu4540 0.23 -0.8 1,687,003 - Atu4540 0.74 -2.2 1,687,134 - -1.1 1,687,311 + Atu4541 0.27 +0.8 1,687,319 + Atu4541 0.29 -1.6 1,687,327 - Atu4541 0.31 -1.0 1,687,356 + Atu4541 0.37 +1.8 1,687,377 + Atu4541 0.42 -0.3 1,687,385 - Atu4541 0.44 +0.2 1,687,559 + Atu4541 0.84 +2.4 1,687,581 + Atu4541 0.89 -0.2 1,687,581 + Atu4541 0.89 -0.9 1,687,669 + +0.8 1,687,687 - +0.1 1,687,690 + -1.4 1,687,690 + +0.4 1,687,715 + Atu4542 0.11 +0.7 1,687,780 + Atu4542 0.19 -2.0 1,687,830 + Atu4542 0.25 -0.1 1,687,848 - Atu4542 0.27 +1.8 1,687,948 + Atu4542 0.40 -2.5 1,687,956 - Atu4542 0.41 +0.0 1,687,956 - Atu4542 0.41 +0.4 1,687,956 - Atu4542 0.41 +0.2 1,687,991 - Atu4542 0.45 +0.7 1,688,039 + Atu4542 0.51 +0.2 1,688,094 + Atu4542 0.58 -1.2
Or see this region's nucleotide sequence