Experiment: Chloramphenicol 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3453 and Atu3454 are separated by 6 nucleotides Atu3454 and Atu3455 are separated by 81 nucleotides Atu3455 and Atu3456 are separated by 217 nucleotides Atu3456 and Atu3457 overlap by 1 nucleotides
Atu3453: Atu3453 - isomerase/lactonizing enzyme, at 500,975 to 502,123
Atu3453
Atu3454: Atu3454 - arylesterase, at 502,130 to 502,759
Atu3454
Atu3455: Atu3455 - oligopeptide ABC transporter substrate-binding protein, at 502,841 to 504,439
Atu3455
Atu3456: Atu3456 - ABC transporter permease, at 504,657 to 505,415
Atu3456
Atu3457: Atu3457 - ABC transporter permease, at 505,415 to 506,251
Atu3457
Position (kb) 502
503
504
505 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 501.843 kb on + strand, within Atu3453 at 501.853 kb on + strand, within Atu3453 at 501.858 kb on - strand, within Atu3453 at 501.962 kb on - strand, within Atu3453 at 501.992 kb on + strand, within Atu3453 at 502.016 kb on - strand at 502.078 kb on - strand at 502.119 kb on + strand at 502.146 kb on + strand at 502.156 kb on + strand at 502.187 kb on + strand at 502.195 kb on - strand, within Atu3454 at 502.195 kb on - strand, within Atu3454 at 502.218 kb on - strand, within Atu3454 at 502.233 kb on - strand, within Atu3454 at 502.351 kb on + strand, within Atu3454 at 502.359 kb on - strand, within Atu3454 at 502.450 kb on + strand, within Atu3454 at 502.539 kb on - strand, within Atu3454 at 502.577 kb on + strand, within Atu3454 at 502.583 kb on - strand, within Atu3454 at 502.583 kb on - strand, within Atu3454 at 502.585 kb on + strand, within Atu3454 at 502.738 kb on + strand at 502.738 kb on + strand at 502.860 kb on + strand at 502.875 kb on - strand at 502.988 kb on - strand at 503.006 kb on + strand, within Atu3455 at 503.014 kb on - strand, within Atu3455 at 503.025 kb on - strand, within Atu3455 at 503.025 kb on - strand, within Atu3455 at 503.058 kb on + strand, within Atu3455 at 503.123 kb on - strand, within Atu3455 at 503.251 kb on + strand, within Atu3455 at 503.308 kb on + strand, within Atu3455 at 503.308 kb on + strand, within Atu3455 at 503.316 kb on - strand, within Atu3455 at 503.427 kb on + strand, within Atu3455 at 503.457 kb on + strand, within Atu3455 at 503.457 kb on + strand, within Atu3455 at 503.466 kb on - strand, within Atu3455 at 503.473 kb on + strand, within Atu3455 at 503.490 kb on + strand, within Atu3455 at 503.590 kb on - strand, within Atu3455 at 503.604 kb on + strand, within Atu3455 at 503.639 kb on - strand, within Atu3455 at 503.784 kb on + strand, within Atu3455 at 503.872 kb on - strand, within Atu3455 at 504.151 kb on - strand, within Atu3455 at 504.157 kb on - strand, within Atu3455 at 504.251 kb on - strand, within Atu3455 at 504.296 kb on - strand at 504.314 kb on - strand at 504.328 kb on - strand at 504.337 kb on - strand at 504.376 kb on + strand at 504.406 kb on - strand at 504.440 kb on + strand at 504.449 kb on - strand at 504.500 kb on + strand at 504.542 kb on + strand at 504.562 kb on - strand at 504.810 kb on - strand, within Atu3456 at 504.820 kb on - strand, within Atu3456 at 504.833 kb on + strand, within Atu3456 at 504.938 kb on + strand, within Atu3456 at 505.008 kb on + strand, within Atu3456 at 505.053 kb on - strand, within Atu3456 at 505.153 kb on - strand, within Atu3456 at 505.187 kb on + strand, within Atu3456 at 505.212 kb on - strand, within Atu3456 at 505.379 kb on - strand at 505.434 kb on - strand at 505.435 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 12.5 ug/mL remove 501,843 + Atu3453 0.76 +0.6 501,853 + Atu3453 0.76 +0.1 501,858 - Atu3453 0.77 -1.0 501,962 - Atu3453 0.86 +0.9 501,992 + Atu3453 0.89 -0.4 502,016 - +0.9 502,078 - -0.2 502,119 + -0.0 502,146 + -0.4 502,156 + +0.6 502,187 + -1.2 502,195 - Atu3454 0.10 +2.1 502,195 - Atu3454 0.10 +0.9 502,218 - Atu3454 0.14 +0.4 502,233 - Atu3454 0.16 +0.4 502,351 + Atu3454 0.35 -0.8 502,359 - Atu3454 0.36 -0.9 502,450 + Atu3454 0.51 -1.4 502,539 - Atu3454 0.65 -0.9 502,577 + Atu3454 0.71 +0.5 502,583 - Atu3454 0.72 +0.5 502,583 - Atu3454 0.72 -1.6 502,585 + Atu3454 0.72 +0.4 502,738 + -1.1 502,738 + +0.3 502,860 + -0.6 502,875 - -2.8 502,988 - -0.7 503,006 + Atu3455 0.10 -0.5 503,014 - Atu3455 0.11 -1.9 503,025 - Atu3455 0.12 +0.4 503,025 - Atu3455 0.12 +0.6 503,058 + Atu3455 0.14 -0.1 503,123 - Atu3455 0.18 +0.7 503,251 + Atu3455 0.26 -0.3 503,308 + Atu3455 0.29 -2.8 503,308 + Atu3455 0.29 +0.1 503,316 - Atu3455 0.30 +0.7 503,427 + Atu3455 0.37 -0.2 503,457 + Atu3455 0.39 +1.7 503,457 + Atu3455 0.39 -0.3 503,466 - Atu3455 0.39 +1.0 503,473 + Atu3455 0.40 -1.5 503,490 + Atu3455 0.41 +1.7 503,590 - Atu3455 0.47 -0.9 503,604 + Atu3455 0.48 -0.9 503,639 - Atu3455 0.50 +2.4 503,784 + Atu3455 0.59 +0.3 503,872 - Atu3455 0.64 +0.7 504,151 - Atu3455 0.82 -0.7 504,157 - Atu3455 0.82 +0.7 504,251 - Atu3455 0.88 -0.6 504,296 - +2.3 504,314 - -0.3 504,328 - -0.1 504,337 - +1.2 504,376 + +0.0 504,406 - -2.5 504,440 + +0.1 504,449 - +0.1 504,500 + -0.3 504,542 + +0.5 504,562 - +0.6 504,810 - Atu3456 0.20 +0.1 504,820 - Atu3456 0.21 +0.4 504,833 + Atu3456 0.23 +0.1 504,938 + Atu3456 0.37 -1.5 505,008 + Atu3456 0.46 -1.7 505,053 - Atu3456 0.52 +0.4 505,153 - Atu3456 0.65 +0.2 505,187 + Atu3456 0.70 +0.4 505,212 - Atu3456 0.73 +0.2 505,379 - -1.5 505,434 - -1.2 505,435 + +1.7
Or see this region's nucleotide sequence