Experiment: Chloramphenicol 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3377 and Atu3378 overlap by 4 nucleotides Atu3378 and Atu3379 overlap by 4 nucleotides Atu3379 and Atu3381 are separated by 180 nucleotides
Atu3377: Atu3377 - peptide ABC transporter ATPase, at 418,936 to 420,570
Atu3377
Atu3378: Atu3378 - oligopeptide ABC transporter permease, at 420,567 to 421,385
Atu3378
Atu3379: Atu3379 - peptide ABC transporter permease, at 421,382 to 422,326
Atu3379
Atu3381: Atu3381 - LysR family transcriptional regulator, at 422,507 to 423,412
Atu3381
Position (kb)
421
422
423 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 420.394 kb on + strand, within Atu3377 at 420.494 kb on + strand at 420.547 kb on + strand at 420.577 kb on + strand at 420.590 kb on + strand at 420.598 kb on - strand at 420.676 kb on - strand, within Atu3378 at 420.746 kb on + strand, within Atu3378 at 420.754 kb on - strand, within Atu3378 at 420.762 kb on + strand, within Atu3378 at 420.832 kb on - strand, within Atu3378 at 420.923 kb on + strand, within Atu3378 at 420.923 kb on + strand, within Atu3378 at 420.926 kb on - strand, within Atu3378 at 420.931 kb on - strand, within Atu3378 at 421.019 kb on + strand, within Atu3378 at 421.231 kb on - strand, within Atu3378 at 421.281 kb on - strand, within Atu3378 at 421.300 kb on + strand, within Atu3378 at 421.300 kb on + strand, within Atu3378 at 421.305 kb on + strand at 421.308 kb on - strand at 421.308 kb on - strand at 421.318 kb on - strand at 421.329 kb on + strand at 421.377 kb on - strand at 421.413 kb on + strand at 421.421 kb on - strand at 421.421 kb on - strand at 421.483 kb on - strand, within Atu3379 at 421.488 kb on + strand, within Atu3379 at 421.541 kb on + strand, within Atu3379 at 421.541 kb on + strand, within Atu3379 at 421.615 kb on + strand, within Atu3379 at 421.641 kb on + strand, within Atu3379 at 421.643 kb on + strand, within Atu3379 at 421.648 kb on + strand, within Atu3379 at 421.780 kb on + strand, within Atu3379 at 421.788 kb on - strand, within Atu3379 at 421.817 kb on + strand, within Atu3379 at 421.902 kb on - strand, within Atu3379 at 421.905 kb on + strand, within Atu3379 at 421.917 kb on - strand, within Atu3379 at 421.923 kb on + strand, within Atu3379 at 421.931 kb on - strand, within Atu3379 at 422.008 kb on - strand, within Atu3379 at 422.115 kb on - strand, within Atu3379 at 422.164 kb on + strand, within Atu3379 at 422.164 kb on + strand, within Atu3379 at 422.172 kb on - strand, within Atu3379 at 422.172 kb on - strand, within Atu3379 at 422.172 kb on - strand, within Atu3379 at 422.178 kb on + strand, within Atu3379 at 422.186 kb on - strand, within Atu3379 at 422.188 kb on + strand, within Atu3379 at 422.197 kb on + strand, within Atu3379 at 422.212 kb on + strand, within Atu3379 at 422.288 kb on - strand at 422.300 kb on - strand at 422.357 kb on + strand at 422.459 kb on + strand at 422.459 kb on + strand at 422.467 kb on - strand at 422.581 kb on - strand at 422.581 kb on - strand at 422.703 kb on - strand, within Atu3381 at 422.716 kb on - strand, within Atu3381 at 422.874 kb on - strand, within Atu3381 at 423.003 kb on + strand, within Atu3381 at 423.016 kb on - strand, within Atu3381 at 423.256 kb on - strand, within Atu3381 at 423.274 kb on - strand, within Atu3381 at 423.299 kb on - strand, within Atu3381
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 12.5 ug/mL remove 420,394 + Atu3377 0.89 +0.2 420,494 + -2.5 420,547 + -2.2 420,577 + +1.2 420,590 + -1.0 420,598 - +0.7 420,676 - Atu3378 0.13 -0.8 420,746 + Atu3378 0.22 -1.1 420,754 - Atu3378 0.23 -0.2 420,762 + Atu3378 0.24 -2.1 420,832 - Atu3378 0.32 -0.1 420,923 + Atu3378 0.43 +1.1 420,923 + Atu3378 0.43 -0.1 420,926 - Atu3378 0.44 -0.8 420,931 - Atu3378 0.44 +1.1 421,019 + Atu3378 0.55 -0.7 421,231 - Atu3378 0.81 +1.7 421,281 - Atu3378 0.87 -0.3 421,300 + Atu3378 0.89 +1.2 421,300 + Atu3378 0.89 +0.2 421,305 + -0.6 421,308 - -0.6 421,308 - +1.2 421,318 - +1.1 421,329 + -0.8 421,377 - -0.8 421,413 + +0.0 421,421 - +0.5 421,421 - +0.2 421,483 - Atu3379 0.11 +3.0 421,488 + Atu3379 0.11 +1.0 421,541 + Atu3379 0.17 -2.1 421,541 + Atu3379 0.17 +0.5 421,615 + Atu3379 0.25 +0.7 421,641 + Atu3379 0.27 -2.6 421,643 + Atu3379 0.28 -1.4 421,648 + Atu3379 0.28 -0.3 421,780 + Atu3379 0.42 +0.0 421,788 - Atu3379 0.43 +0.6 421,817 + Atu3379 0.46 -0.2 421,902 - Atu3379 0.55 -0.3 421,905 + Atu3379 0.55 -2.0 421,917 - Atu3379 0.57 +2.1 421,923 + Atu3379 0.57 -0.3 421,931 - Atu3379 0.58 -0.7 422,008 - Atu3379 0.66 +1.7 422,115 - Atu3379 0.78 +0.1 422,164 + Atu3379 0.83 -0.2 422,164 + Atu3379 0.83 -0.6 422,172 - Atu3379 0.84 -1.3 422,172 - Atu3379 0.84 -1.8 422,172 - Atu3379 0.84 +0.9 422,178 + Atu3379 0.84 +2.3 422,186 - Atu3379 0.85 +1.7 422,188 + Atu3379 0.85 -0.3 422,197 + Atu3379 0.86 +0.6 422,212 + Atu3379 0.88 +0.2 422,288 - -2.2 422,300 - +0.6 422,357 + +1.1 422,459 + -0.2 422,459 + -0.3 422,467 - +0.1 422,581 - +0.6 422,581 - +0.8 422,703 - Atu3381 0.22 +1.2 422,716 - Atu3381 0.23 -2.5 422,874 - Atu3381 0.41 -0.0 423,003 + Atu3381 0.55 -0.4 423,016 - Atu3381 0.56 +0.1 423,256 - Atu3381 0.83 +0.3 423,274 - Atu3381 0.85 -1.6 423,299 - Atu3381 0.87 +0.5
Or see this region's nucleotide sequence