Experiment: Chloramphenicol 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3140 and Atu3141 are separated by 113 nucleotides Atu3141 and Atu3142 overlap by 4 nucleotides Atu3142 and Atu3143 are separated by 138 nucleotides
Atu3140: Atu3140 - 5-dehydro-4-deoxyglucarate dehydratase, at 148,144 to 149,055
Atu3140
Atu3141: Atu3141 - 2-deoxy-D-gluconate 3-dehydrogenase, at 149,169 to 149,912
Atu3141
Atu3142: Atu3142 - 5-keto-4-deoxyuronate isomerase, at 149,909 to 150,733
Atu3142
Atu3143: Atu3143 - hypothetical protein, at 150,872 to 151,669
Atu3143
Position (kb)
149
150
151 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 148.939 kb on + strand, within Atu3140 at 148.958 kb on - strand, within Atu3140 at 149.043 kb on + strand at 149.056 kb on - strand at 149.056 kb on - strand at 149.209 kb on - strand at 149.316 kb on - strand, within Atu3141 at 149.392 kb on - strand, within Atu3141 at 149.455 kb on + strand, within Atu3141 at 149.455 kb on + strand, within Atu3141 at 149.455 kb on + strand, within Atu3141 at 149.463 kb on - strand, within Atu3141 at 149.689 kb on + strand, within Atu3141 at 149.692 kb on - strand, within Atu3141 at 149.765 kb on + strand, within Atu3141 at 149.771 kb on - strand, within Atu3141 at 149.797 kb on + strand, within Atu3141 at 149.797 kb on + strand, within Atu3141 at 149.805 kb on - strand, within Atu3141 at 149.815 kb on - strand, within Atu3141 at 149.831 kb on - strand, within Atu3141 at 149.920 kb on - strand at 149.964 kb on - strand at 149.978 kb on + strand at 150.173 kb on + strand, within Atu3142 at 150.173 kb on + strand, within Atu3142 at 150.201 kb on + strand, within Atu3142 at 150.201 kb on + strand, within Atu3142 at 150.201 kb on + strand, within Atu3142 at 150.209 kb on - strand, within Atu3142 at 150.209 kb on - strand, within Atu3142 at 150.214 kb on - strand, within Atu3142 at 150.261 kb on - strand, within Atu3142 at 150.291 kb on + strand, within Atu3142 at 150.325 kb on - strand, within Atu3142 at 150.351 kb on + strand, within Atu3142 at 150.359 kb on - strand, within Atu3142 at 150.359 kb on - strand, within Atu3142 at 150.363 kb on + strand, within Atu3142 at 150.373 kb on + strand, within Atu3142 at 150.381 kb on - strand, within Atu3142 at 150.422 kb on - strand, within Atu3142 at 150.564 kb on + strand, within Atu3142 at 150.564 kb on + strand, within Atu3142 at 150.572 kb on - strand, within Atu3142 at 150.572 kb on - strand, within Atu3142 at 150.589 kb on - strand, within Atu3142 at 150.604 kb on - strand, within Atu3142 at 150.606 kb on + strand, within Atu3142 at 150.606 kb on + strand, within Atu3142 at 150.606 kb on + strand, within Atu3142 at 150.614 kb on - strand, within Atu3142 at 150.614 kb on - strand, within Atu3142 at 150.614 kb on - strand, within Atu3142 at 150.614 kb on - strand, within Atu3142 at 150.630 kb on + strand, within Atu3142 at 150.675 kb on - strand at 150.817 kb on + strand at 150.817 kb on - strand at 150.835 kb on - strand at 150.835 kb on - strand at 150.837 kb on + strand at 150.930 kb on + strand at 150.970 kb on + strand, within Atu3143 at 150.992 kb on - strand, within Atu3143 at 151.065 kb on + strand, within Atu3143 at 151.073 kb on - strand, within Atu3143 at 151.128 kb on - strand, within Atu3143 at 151.164 kb on + strand, within Atu3143 at 151.172 kb on - strand, within Atu3143 at 151.223 kb on - strand, within Atu3143 at 151.247 kb on - strand, within Atu3143 at 151.337 kb on - strand, within Atu3143 at 151.454 kb on + strand, within Atu3143 at 151.546 kb on + strand, within Atu3143 at 151.546 kb on + strand, within Atu3143 at 151.554 kb on - strand, within Atu3143 at 151.570 kb on + strand, within Atu3143 at 151.621 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 12.5 ug/mL remove 148,939 + Atu3140 0.87 +1.2 148,958 - Atu3140 0.89 -1.0 149,043 + -1.3 149,056 - -0.0 149,056 - +1.0 149,209 - -1.2 149,316 - Atu3141 0.20 +0.7 149,392 - Atu3141 0.30 +0.1 149,455 + Atu3141 0.38 -1.8 149,455 + Atu3141 0.38 -1.8 149,455 + Atu3141 0.38 -0.6 149,463 - Atu3141 0.40 -1.0 149,689 + Atu3141 0.70 -1.6 149,692 - Atu3141 0.70 +0.7 149,765 + Atu3141 0.80 +0.1 149,771 - Atu3141 0.81 -0.8 149,797 + Atu3141 0.84 -0.2 149,797 + Atu3141 0.84 -0.1 149,805 - Atu3141 0.85 -1.6 149,815 - Atu3141 0.87 -0.3 149,831 - Atu3141 0.89 -0.2 149,920 - +2.8 149,964 - -1.9 149,978 + +0.4 150,173 + Atu3142 0.32 -1.2 150,173 + Atu3142 0.32 +0.1 150,201 + Atu3142 0.35 +0.0 150,201 + Atu3142 0.35 -0.6 150,201 + Atu3142 0.35 -0.5 150,209 - Atu3142 0.36 +0.1 150,209 - Atu3142 0.36 -0.8 150,214 - Atu3142 0.37 -0.2 150,261 - Atu3142 0.43 -0.3 150,291 + Atu3142 0.46 -1.5 150,325 - Atu3142 0.50 -2.4 150,351 + Atu3142 0.54 -2.6 150,359 - Atu3142 0.55 -0.5 150,359 - Atu3142 0.55 -1.2 150,363 + Atu3142 0.55 +0.0 150,373 + Atu3142 0.56 +0.5 150,381 - Atu3142 0.57 -0.1 150,422 - Atu3142 0.62 -0.2 150,564 + Atu3142 0.79 +0.1 150,564 + Atu3142 0.79 +0.7 150,572 - Atu3142 0.80 -1.0 150,572 - Atu3142 0.80 -0.5 150,589 - Atu3142 0.82 +1.8 150,604 - Atu3142 0.84 -0.9 150,606 + Atu3142 0.84 -0.0 150,606 + Atu3142 0.84 -1.2 150,606 + Atu3142 0.84 -0.3 150,614 - Atu3142 0.85 -1.0 150,614 - Atu3142 0.85 +0.4 150,614 - Atu3142 0.85 +0.0 150,614 - Atu3142 0.85 -1.0 150,630 + Atu3142 0.87 +0.8 150,675 - +0.4 150,817 + +1.8 150,817 - +0.2 150,835 - +0.5 150,835 - -0.3 150,837 + +0.8 150,930 + +0.4 150,970 + Atu3143 0.12 -0.0 150,992 - Atu3143 0.15 +1.5 151,065 + Atu3143 0.24 +0.8 151,073 - Atu3143 0.25 -1.7 151,128 - Atu3143 0.32 -0.5 151,164 + Atu3143 0.37 +0.8 151,172 - Atu3143 0.38 +1.3 151,223 - Atu3143 0.44 -0.1 151,247 - Atu3143 0.47 +0.6 151,337 - Atu3143 0.58 +0.2 151,454 + Atu3143 0.73 -1.1 151,546 + Atu3143 0.84 +1.1 151,546 + Atu3143 0.84 +1.2 151,554 - Atu3143 0.85 -0.2 151,570 + Atu3143 0.87 -1.2 151,621 + -1.7
Or see this region's nucleotide sequence