Experiment: Chloramphenicol 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3096 and Atu3097 are separated by 82 nucleotides Atu3097 and Atu3098 are separated by 7 nucleotides Atu3098 and Atu3099 are separated by 31 nucleotides
Atu3096: Atu3096 - sn-glycerol 3-phosphate ABC transporter substrate-binding protein, at 102,956 to 104,299
Atu3096
Atu3097: Atu3097 - sn-glycerol-3-phosphate ABC transporter membrane protein, at 104,382 to 105,272
Atu3097
Atu3098: Atu3098 - sn-glycerol-3-phosphate ABC transporter membrane protein, at 105,280 to 106,164
Atu3098
Atu3099: Atu3099 - glycerol-3-phosphate transporter ATP-binding subunit, at 106,196 to 107,317
Atu3099
Position (kb)
105
106
107 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 104.290 kb on + strand at 104.326 kb on - strand at 104.326 kb on - strand at 104.346 kb on - strand at 104.346 kb on - strand at 104.449 kb on + strand at 104.492 kb on - strand, within Atu3097 at 104.492 kb on - strand, within Atu3097 at 104.642 kb on + strand, within Atu3097 at 104.652 kb on + strand, within Atu3097 at 104.652 kb on + strand, within Atu3097 at 104.713 kb on - strand, within Atu3097 at 104.759 kb on - strand, within Atu3097 at 104.847 kb on + strand, within Atu3097 at 104.852 kb on + strand, within Atu3097 at 104.895 kb on + strand, within Atu3097 at 104.897 kb on - strand, within Atu3097 at 104.963 kb on - strand, within Atu3097 at 104.965 kb on + strand, within Atu3097 at 104.973 kb on - strand, within Atu3097 at 104.992 kb on + strand, within Atu3097 at 105.013 kb on + strand, within Atu3097 at 105.106 kb on + strand, within Atu3097 at 105.186 kb on + strand at 105.194 kb on - strand at 105.219 kb on + strand at 105.295 kb on + strand at 105.295 kb on + strand at 105.295 kb on + strand at 105.303 kb on - strand at 105.315 kb on - strand at 105.343 kb on - strand at 105.389 kb on - strand, within Atu3098 at 105.399 kb on + strand, within Atu3098 at 105.399 kb on + strand, within Atu3098 at 105.420 kb on - strand, within Atu3098 at 105.420 kb on - strand, within Atu3098 at 105.422 kb on - strand, within Atu3098 at 105.498 kb on - strand, within Atu3098 at 105.498 kb on - strand, within Atu3098 at 105.600 kb on + strand, within Atu3098 at 105.649 kb on + strand, within Atu3098 at 105.649 kb on + strand, within Atu3098 at 105.657 kb on - strand, within Atu3098 at 105.657 kb on - strand, within Atu3098 at 105.657 kb on - strand, within Atu3098 at 105.695 kb on - strand, within Atu3098 at 105.716 kb on - strand, within Atu3098 at 105.716 kb on - strand, within Atu3098 at 105.733 kb on - strand, within Atu3098 at 105.757 kb on + strand, within Atu3098 at 105.958 kb on + strand, within Atu3098 at 105.975 kb on + strand, within Atu3098 at 106.014 kb on - strand, within Atu3098 at 106.179 kb on - strand at 106.247 kb on + strand at 106.257 kb on + strand at 106.257 kb on + strand at 106.265 kb on - strand at 106.347 kb on + strand, within Atu3099 at 106.393 kb on + strand, within Atu3099 at 106.401 kb on + strand, within Atu3099 at 106.409 kb on - strand, within Atu3099 at 106.427 kb on + strand, within Atu3099 at 106.580 kb on - strand, within Atu3099 at 106.622 kb on + strand, within Atu3099 at 106.630 kb on - strand, within Atu3099 at 106.678 kb on + strand, within Atu3099 at 106.678 kb on + strand, within Atu3099 at 106.689 kb on + strand, within Atu3099 at 106.817 kb on + strand, within Atu3099 at 106.822 kb on + strand, within Atu3099 at 106.830 kb on - strand, within Atu3099 at 106.879 kb on - strand, within Atu3099 at 106.952 kb on - strand, within Atu3099 at 106.981 kb on - strand, within Atu3099 at 107.034 kb on + strand, within Atu3099 at 107.055 kb on + strand, within Atu3099 at 107.125 kb on + strand, within Atu3099
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 12.5 ug/mL remove 104,290 + -0.2 104,326 - +2.8 104,326 - +0.4 104,346 - +0.2 104,346 - +1.1 104,449 + -0.4 104,492 - Atu3097 0.12 -0.8 104,492 - Atu3097 0.12 -1.6 104,642 + Atu3097 0.29 -0.3 104,652 + Atu3097 0.30 +1.2 104,652 + Atu3097 0.30 +0.5 104,713 - Atu3097 0.37 -0.2 104,759 - Atu3097 0.42 +1.9 104,847 + Atu3097 0.52 -1.0 104,852 + Atu3097 0.53 -1.1 104,895 + Atu3097 0.58 +3.8 104,897 - Atu3097 0.58 +0.8 104,963 - Atu3097 0.65 -1.8 104,965 + Atu3097 0.65 -1.0 104,973 - Atu3097 0.66 -1.7 104,992 + Atu3097 0.68 -0.5 105,013 + Atu3097 0.71 -1.5 105,106 + Atu3097 0.81 -0.3 105,186 + -0.5 105,194 - +0.2 105,219 + -1.3 105,295 + +0.2 105,295 + +1.8 105,295 + -0.5 105,303 - -0.0 105,315 - -0.4 105,343 - +0.4 105,389 - Atu3098 0.12 +0.8 105,399 + Atu3098 0.13 +1.0 105,399 + Atu3098 0.13 -1.5 105,420 - Atu3098 0.16 -0.5 105,420 - Atu3098 0.16 -0.5 105,422 - Atu3098 0.16 -0.2 105,498 - Atu3098 0.25 -0.8 105,498 - Atu3098 0.25 -0.8 105,600 + Atu3098 0.36 -1.9 105,649 + Atu3098 0.42 +0.1 105,649 + Atu3098 0.42 -0.3 105,657 - Atu3098 0.43 -0.5 105,657 - Atu3098 0.43 +0.5 105,657 - Atu3098 0.43 +0.2 105,695 - Atu3098 0.47 +0.1 105,716 - Atu3098 0.49 -0.2 105,716 - Atu3098 0.49 -2.3 105,733 - Atu3098 0.51 -0.5 105,757 + Atu3098 0.54 +0.7 105,958 + Atu3098 0.77 +0.1 105,975 + Atu3098 0.79 -1.3 106,014 - Atu3098 0.83 -0.4 106,179 - +0.6 106,247 + -0.3 106,257 + -0.8 106,257 + -0.8 106,265 - +1.2 106,347 + Atu3099 0.13 -0.1 106,393 + Atu3099 0.18 -0.8 106,401 + Atu3099 0.18 +2.4 106,409 - Atu3099 0.19 +0.6 106,427 + Atu3099 0.21 +0.3 106,580 - Atu3099 0.34 +1.1 106,622 + Atu3099 0.38 +1.4 106,630 - Atu3099 0.39 +0.3 106,678 + Atu3099 0.43 -1.5 106,678 + Atu3099 0.43 +1.0 106,689 + Atu3099 0.44 +1.1 106,817 + Atu3099 0.55 +0.7 106,822 + Atu3099 0.56 +0.5 106,830 - Atu3099 0.57 +0.5 106,879 - Atu3099 0.61 -1.6 106,952 - Atu3099 0.67 +0.8 106,981 - Atu3099 0.70 +0.4 107,034 + Atu3099 0.75 -0.3 107,055 + Atu3099 0.77 -0.2 107,125 + Atu3099 0.83 -1.3
Or see this region's nucleotide sequence