Experiment: Chloramphenicol 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2038 and Atu2039 are separated by 7 nucleotides Atu2039 and Atu2040 overlap by 2 nucleotides Atu2040 and Atu2041 overlap by 4 nucleotides Atu2041 and Atu2042 are separated by 25 nucleotides
Atu2038: Atu2038 - hypothetical protein, at 1,996,183 to 1,998,189
Atu2038
Atu2039: Atu2039 - tRNA delta(2)-isopentenylpyrophosphate, at 1,998,197 to 1,999,093
Atu2039
Atu2040: Atu2040 - phosphoserine phosphatase, at 1,999,092 to 1,999,982
Atu2040
Atu2041: Atu2041 - acetyltransferase, at 1,999,979 to 2,000,542
Atu2041
Atu2042: Atu2042 - short chain dehydrogenase, at 2,000,568 to 2,001,317
Atu2042
Position (kb)
1999
2000 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1998.102 kb on + strand at 1998.192 kb on - strand at 1998.192 kb on - strand at 1998.193 kb on - strand at 1998.474 kb on - strand, within Atu2039 at 1999.227 kb on - strand, within Atu2040 at 1999.418 kb on + strand, within Atu2040 at 1999.427 kb on - strand, within Atu2040 at 1999.493 kb on + strand, within Atu2040 at 1999.493 kb on + strand, within Atu2040 at 1999.493 kb on + strand, within Atu2040 at 1999.501 kb on - strand, within Atu2040 at 1999.501 kb on - strand, within Atu2040 at 1999.844 kb on + strand, within Atu2040 at 1999.863 kb on - strand, within Atu2040 at 1999.899 kb on + strand at 1999.923 kb on - strand at 2000.016 kb on + strand at 2000.031 kb on + strand at 2000.211 kb on - strand, within Atu2041 at 2000.466 kb on - strand, within Atu2041 at 2000.743 kb on + strand, within Atu2042 at 2000.744 kb on + strand, within Atu2042 at 2000.817 kb on - strand, within Atu2042 at 2000.831 kb on - strand, within Atu2042 at 2000.885 kb on - strand, within Atu2042
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 12.5 ug/mL remove 1,998,102 + +0.5 1,998,192 - -0.6 1,998,192 - +3.0 1,998,193 - +0.9 1,998,474 - Atu2039 0.31 +0.0 1,999,227 - Atu2040 0.15 -0.4 1,999,418 + Atu2040 0.37 -1.9 1,999,427 - Atu2040 0.38 -1.0 1,999,493 + Atu2040 0.45 -1.2 1,999,493 + Atu2040 0.45 -1.6 1,999,493 + Atu2040 0.45 -0.2 1,999,501 - Atu2040 0.46 +1.2 1,999,501 - Atu2040 0.46 -1.1 1,999,844 + Atu2040 0.84 +0.6 1,999,863 - Atu2040 0.87 -1.7 1,999,899 + -0.1 1,999,923 - -1.9 2,000,016 + -0.1 2,000,031 + +0.5 2,000,211 - Atu2041 0.41 +0.4 2,000,466 - Atu2041 0.86 -0.5 2,000,743 + Atu2042 0.23 -0.9 2,000,744 + Atu2042 0.23 +0.0 2,000,817 - Atu2042 0.33 -0.4 2,000,831 - Atu2042 0.35 +0.0 2,000,885 - Atu2042 0.42 -0.3
Or see this region's nucleotide sequence