Experiment: Chloramphenicol 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0157 and Atu0158 are separated by 138 nucleotides Atu0158 and Atu0159 overlap by 1 nucleotides Atu0159 and Atu0160 are separated by 34 nucleotides
Atu0157: Atu0157 - ABC transporter, substrate binding protein, at 163,006 to 164,007
Atu0157
Atu0158: Atu0158 - ABC transporter, membrane spanning protein, at 164,146 to 164,919
Atu0158
Atu0159: Atu0159 - ABC transporter, nucleotide binding/ATPase protein, at 164,919 to 165,704
Atu0159
Atu0160: Atu0160 - transcriptional regulator, GntR family, at 165,739 to 166,476
Atu0160
Position (kb)
164
165
166 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 163.959 kb on + strand at 163.993 kb on + strand at 163.994 kb on - strand at 164.073 kb on - strand at 164.084 kb on + strand at 164.153 kb on + strand at 164.155 kb on + strand at 164.155 kb on + strand at 164.164 kb on + strand at 164.164 kb on + strand at 164.221 kb on + strand at 164.221 kb on + strand at 164.359 kb on - strand, within Atu0158 at 164.427 kb on + strand, within Atu0158 at 164.447 kb on - strand, within Atu0158 at 164.480 kb on - strand, within Atu0158 at 164.491 kb on + strand, within Atu0158 at 164.613 kb on + strand, within Atu0158 at 164.621 kb on - strand, within Atu0158 at 164.621 kb on - strand, within Atu0158 at 164.624 kb on + strand, within Atu0158 at 164.624 kb on + strand, within Atu0158 at 164.624 kb on + strand, within Atu0158 at 164.656 kb on - strand, within Atu0158 at 164.719 kb on + strand, within Atu0158 at 164.724 kb on + strand, within Atu0158 at 164.725 kb on + strand, within Atu0158 at 164.733 kb on - strand, within Atu0158 at 164.744 kb on - strand, within Atu0158 at 164.813 kb on - strand, within Atu0158 at 164.848 kb on + strand at 164.857 kb on - strand at 164.896 kb on - strand at 165.043 kb on + strand, within Atu0159 at 165.131 kb on + strand, within Atu0159 at 165.158 kb on + strand, within Atu0159 at 165.173 kb on + strand, within Atu0159 at 165.175 kb on - strand, within Atu0159 at 165.183 kb on + strand, within Atu0159 at 165.337 kb on - strand, within Atu0159 at 165.460 kb on + strand, within Atu0159 at 165.460 kb on + strand, within Atu0159 at 165.460 kb on + strand, within Atu0159 at 165.460 kb on + strand, within Atu0159 at 165.468 kb on - strand, within Atu0159 at 165.468 kb on - strand, within Atu0159 at 165.468 kb on - strand, within Atu0159 at 165.468 kb on - strand, within Atu0159 at 165.472 kb on - strand, within Atu0159 at 165.527 kb on - strand, within Atu0159 at 165.527 kb on - strand, within Atu0159 at 165.532 kb on + strand, within Atu0159 at 165.605 kb on + strand, within Atu0159 at 165.615 kb on + strand, within Atu0159 at 165.632 kb on - strand at 165.647 kb on + strand at 165.739 kb on - strand at 165.799 kb on + strand at 165.818 kb on - strand, within Atu0160 at 165.839 kb on + strand, within Atu0160 at 165.885 kb on + strand, within Atu0160 at 165.926 kb on - strand, within Atu0160 at 165.933 kb on + strand, within Atu0160 at 166.046 kb on - strand, within Atu0160 at 166.069 kb on - strand, within Atu0160 at 166.107 kb on - strand, within Atu0160 at 166.137 kb on + strand, within Atu0160 at 166.282 kb on + strand, within Atu0160 at 166.452 kb on + strand at 166.452 kb on + strand at 166.700 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 12.5 ug/mL remove 163,959 + -0.7 163,993 + +1.4 163,994 - +1.3 164,073 - -1.2 164,084 + -1.6 164,153 + +0.4 164,155 + -1.5 164,155 + -3.5 164,164 + +0.4 164,164 + +0.3 164,221 + +0.7 164,221 + -0.6 164,359 - Atu0158 0.28 +0.5 164,427 + Atu0158 0.36 +1.1 164,447 - Atu0158 0.39 -0.5 164,480 - Atu0158 0.43 -1.4 164,491 + Atu0158 0.45 -0.5 164,613 + Atu0158 0.60 -2.2 164,621 - Atu0158 0.61 -1.8 164,621 - Atu0158 0.61 +0.1 164,624 + Atu0158 0.62 -0.4 164,624 + Atu0158 0.62 +1.8 164,624 + Atu0158 0.62 -3.4 164,656 - Atu0158 0.66 -2.3 164,719 + Atu0158 0.74 -0.1 164,724 + Atu0158 0.75 +0.4 164,725 + Atu0158 0.75 -0.2 164,733 - Atu0158 0.76 +0.1 164,744 - Atu0158 0.77 +0.2 164,813 - Atu0158 0.86 +0.2 164,848 + +0.2 164,857 - +1.3 164,896 - -0.4 165,043 + Atu0159 0.16 +0.1 165,131 + Atu0159 0.27 +0.4 165,158 + Atu0159 0.30 -1.2 165,173 + Atu0159 0.32 -0.2 165,175 - Atu0159 0.33 +2.0 165,183 + Atu0159 0.34 +0.6 165,337 - Atu0159 0.53 -0.6 165,460 + Atu0159 0.69 +0.2 165,460 + Atu0159 0.69 -0.3 165,460 + Atu0159 0.69 -1.1 165,460 + Atu0159 0.69 -0.7 165,468 - Atu0159 0.70 -0.3 165,468 - Atu0159 0.70 -0.3 165,468 - Atu0159 0.70 +0.0 165,468 - Atu0159 0.70 -0.6 165,472 - Atu0159 0.70 -2.4 165,527 - Atu0159 0.77 -0.8 165,527 - Atu0159 0.77 -1.6 165,532 + Atu0159 0.78 -0.5 165,605 + Atu0159 0.87 -0.2 165,615 + Atu0159 0.89 -2.3 165,632 - +0.6 165,647 + -3.3 165,739 - +0.2 165,799 + -0.6 165,818 - Atu0160 0.11 -0.2 165,839 + Atu0160 0.14 +0.6 165,885 + Atu0160 0.20 -0.0 165,926 - Atu0160 0.25 -0.7 165,933 + Atu0160 0.26 -0.6 166,046 - Atu0160 0.42 -1.4 166,069 - Atu0160 0.45 +0.6 166,107 - Atu0160 0.50 +0.3 166,137 + Atu0160 0.54 +1.8 166,282 + Atu0160 0.74 -0.6 166,452 + +1.4 166,452 + -0.4 166,700 + -0.5
Or see this region's nucleotide sequence