Experiment: Hygromycin B 25 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2044 and Atu2045 are separated by 1 nucleotides Atu2045 and Atu2046 are separated by 143 nucleotides Atu2046 and Atu2047 overlap by 4 nucleotides
Atu2044: Atu2044 - HFLC protein, at 2,003,144 to 2,004,067
Atu2044
Atu2045: Atu2045 - HFLK protein, at 2,004,069 to 2,005,187
Atu2045
Atu2046: Atu2046 - dihydrofolate reductase, at 2,005,331 to 2,005,858
Atu2046
Atu2047: Atu2047 - thymidylate synthase, at 2,005,855 to 2,006,649
Atu2047
Position (kb)
2004
2005
2006 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2003.071 kb on + strand at 2003.072 kb on - strand at 2003.079 kb on - strand at 2003.086 kb on - strand at 2003.169 kb on - strand at 2003.174 kb on - strand at 2003.253 kb on - strand, within Atu2044 at 2003.395 kb on - strand, within Atu2044 at 2003.441 kb on - strand, within Atu2044 at 2003.452 kb on + strand, within Atu2044 at 2003.524 kb on - strand, within Atu2044 at 2003.625 kb on - strand, within Atu2044 at 2003.737 kb on - strand, within Atu2044 at 2003.951 kb on - strand, within Atu2044 at 2003.956 kb on - strand, within Atu2044 at 2004.177 kb on + strand at 2004.233 kb on - strand, within Atu2045 at 2004.243 kb on - strand, within Atu2045 at 2004.345 kb on - strand, within Atu2045 at 2004.508 kb on - strand, within Atu2045 at 2004.696 kb on - strand, within Atu2045 at 2004.916 kb on + strand, within Atu2045 at 2005.262 kb on + strand at 2005.281 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hygromycin B 25 ug/mL remove 2,003,071 + -0.3 2,003,072 - +0.2 2,003,079 - -0.5 2,003,086 - +0.2 2,003,169 - -0.7 2,003,174 - -2.1 2,003,253 - Atu2044 0.12 -1.3 2,003,395 - Atu2044 0.27 -0.3 2,003,441 - Atu2044 0.32 +2.0 2,003,452 + Atu2044 0.33 -1.7 2,003,524 - Atu2044 0.41 -1.5 2,003,625 - Atu2044 0.52 -1.5 2,003,737 - Atu2044 0.64 -1.0 2,003,951 - Atu2044 0.87 -0.3 2,003,956 - Atu2044 0.88 -0.1 2,004,177 + -2.2 2,004,233 - Atu2045 0.15 -1.8 2,004,243 - Atu2045 0.16 -0.7 2,004,345 - Atu2045 0.25 -1.8 2,004,508 - Atu2045 0.39 -0.8 2,004,696 - Atu2045 0.56 -0.8 2,004,916 + Atu2045 0.76 +1.6 2,005,262 + +0.1 2,005,281 - -1.0
Or see this region's nucleotide sequence