Experiment: Hygromycin B 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5503 and Atu5504 are separated by 185 nucleotides Atu5504 and Atu5505 are separated by 3 nucleotides Atu5505 and Atu5506 overlap by 4 nucleotides
Atu5503: Atu5503 - hypothetical protein, at 494,054 to 495,511
Atu5503
Atu5504: Atu5504 - hypothetical protein, at 495,697 to 496,290
Atu5504
Atu5505: Atu5505 - two component response regulator, at 496,294 to 496,959
Atu5505
Atu5506: Atu5506 - two component sensor kinase, at 496,956 to 498,314
Atu5506
Position (kb)
495
496
497 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 494.766 kb on - strand, within Atu5503 at 494.771 kb on - strand, within Atu5503 at 494.771 kb on - strand, within Atu5503 at 494.783 kb on + strand, within Atu5503 at 494.810 kb on - strand, within Atu5503 at 494.821 kb on + strand, within Atu5503 at 494.900 kb on - strand, within Atu5503 at 494.903 kb on - strand, within Atu5503 at 494.939 kb on + strand, within Atu5503 at 494.963 kb on - strand, within Atu5503 at 494.974 kb on + strand, within Atu5503 at 494.982 kb on - strand, within Atu5503 at 495.062 kb on + strand, within Atu5503 at 495.072 kb on + strand, within Atu5503 at 495.072 kb on + strand, within Atu5503 at 495.072 kb on + strand, within Atu5503 at 495.153 kb on + strand, within Atu5503 at 495.158 kb on + strand, within Atu5503 at 495.158 kb on + strand, within Atu5503 at 495.161 kb on - strand, within Atu5503 at 495.161 kb on - strand, within Atu5503 at 495.166 kb on - strand, within Atu5503 at 495.245 kb on + strand, within Atu5503 at 495.253 kb on - strand, within Atu5503 at 495.388 kb on + strand at 495.388 kb on + strand at 495.497 kb on - strand at 495.497 kb on - strand at 495.569 kb on - strand at 495.589 kb on - strand at 495.589 kb on - strand at 495.645 kb on + strand at 495.653 kb on - strand at 495.702 kb on - strand at 495.709 kb on + strand at 495.754 kb on + strand at 495.762 kb on - strand, within Atu5504 at 495.849 kb on - strand, within Atu5504 at 495.870 kb on - strand, within Atu5504 at 496.081 kb on + strand, within Atu5504 at 496.081 kb on + strand, within Atu5504 at 496.110 kb on - strand, within Atu5504 at 496.184 kb on - strand, within Atu5504 at 496.292 kb on - strand at 496.323 kb on + strand at 496.324 kb on + strand at 496.333 kb on - strand at 496.338 kb on - strand at 496.370 kb on + strand, within Atu5505 at 496.453 kb on - strand, within Atu5505 at 496.468 kb on + strand, within Atu5505 at 496.543 kb on - strand, within Atu5505 at 496.544 kb on + strand, within Atu5505 at 496.599 kb on + strand, within Atu5505 at 496.607 kb on - strand, within Atu5505 at 496.638 kb on + strand, within Atu5505 at 496.638 kb on + strand, within Atu5505 at 496.681 kb on + strand, within Atu5505 at 496.689 kb on - strand, within Atu5505 at 496.734 kb on - strand, within Atu5505 at 496.784 kb on + strand, within Atu5505 at 496.792 kb on - strand, within Atu5505 at 496.796 kb on - strand, within Atu5505 at 496.851 kb on + strand, within Atu5505 at 496.859 kb on - strand, within Atu5505 at 496.874 kb on - strand, within Atu5505 at 496.906 kb on - strand at 496.924 kb on - strand at 496.932 kb on - strand at 496.934 kb on - strand at 496.944 kb on - strand at 497.080 kb on + strand at 497.088 kb on - strand at 497.117 kb on - strand, within Atu5506 at 497.151 kb on + strand, within Atu5506 at 497.222 kb on + strand, within Atu5506 at 497.223 kb on - strand, within Atu5506 at 497.279 kb on - strand, within Atu5506
Per-strain Table
Position Strand Gene LocusTag Fraction Hygromycin B 12.5 ug/mL remove 494,766 - Atu5503 0.49 +0.6 494,771 - Atu5503 0.49 -0.7 494,771 - Atu5503 0.49 +0.7 494,783 + Atu5503 0.50 +1.6 494,810 - Atu5503 0.52 +0.7 494,821 + Atu5503 0.53 +2.2 494,900 - Atu5503 0.58 -0.5 494,903 - Atu5503 0.58 +0.7 494,939 + Atu5503 0.61 +0.4 494,963 - Atu5503 0.62 +0.2 494,974 + Atu5503 0.63 -0.4 494,982 - Atu5503 0.64 +0.3 495,062 + Atu5503 0.69 -0.0 495,072 + Atu5503 0.70 -0.2 495,072 + Atu5503 0.70 +0.4 495,072 + Atu5503 0.70 -2.1 495,153 + Atu5503 0.75 -0.5 495,158 + Atu5503 0.76 -0.1 495,158 + Atu5503 0.76 +0.3 495,161 - Atu5503 0.76 +0.2 495,161 - Atu5503 0.76 +0.2 495,166 - Atu5503 0.76 -0.2 495,245 + Atu5503 0.82 +0.2 495,253 - Atu5503 0.82 -0.1 495,388 + -0.4 495,388 + +1.6 495,497 - -0.6 495,497 - -0.9 495,569 - +0.1 495,589 - +0.4 495,589 - +0.2 495,645 + +1.3 495,653 - +0.3 495,702 - -0.5 495,709 + -0.2 495,754 + +0.3 495,762 - Atu5504 0.11 +0.3 495,849 - Atu5504 0.26 +0.3 495,870 - Atu5504 0.29 +0.9 496,081 + Atu5504 0.65 +0.1 496,081 + Atu5504 0.65 +0.1 496,110 - Atu5504 0.70 +1.6 496,184 - Atu5504 0.82 -1.1 496,292 - -0.2 496,323 + +1.2 496,324 + -0.6 496,333 - +0.0 496,338 - -0.1 496,370 + Atu5505 0.11 +0.3 496,453 - Atu5505 0.24 +0.6 496,468 + Atu5505 0.26 -0.4 496,543 - Atu5505 0.37 +0.4 496,544 + Atu5505 0.38 -1.1 496,599 + Atu5505 0.46 +0.6 496,607 - Atu5505 0.47 +0.1 496,638 + Atu5505 0.52 +0.4 496,638 + Atu5505 0.52 +0.3 496,681 + Atu5505 0.58 -0.5 496,689 - Atu5505 0.59 -0.1 496,734 - Atu5505 0.66 +0.0 496,784 + Atu5505 0.74 -0.0 496,792 - Atu5505 0.75 +0.4 496,796 - Atu5505 0.75 +1.6 496,851 + Atu5505 0.84 -0.5 496,859 - Atu5505 0.85 +0.6 496,874 - Atu5505 0.87 -1.2 496,906 - +0.8 496,924 - -0.2 496,932 - +1.3 496,934 - -0.3 496,944 - -0.1 497,080 + +1.3 497,088 - -1.0 497,117 - Atu5506 0.12 -0.1 497,151 + Atu5506 0.14 +0.8 497,222 + Atu5506 0.20 +0.6 497,223 - Atu5506 0.20 +0.0 497,279 - Atu5506 0.24 -0.6
Or see this region's nucleotide sequence