Experiment: Hygromycin B 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3625 and Atu3626 are separated by 5 nucleotides Atu3626 and Atu3627 overlap by 4 nucleotides Atu3627 and Atu3628 are separated by 32 nucleotides Atu3628 and Atu3629 overlap by 4 nucleotides
Atu3625: Atu3625 - 3-dehydroquinate synthase, at 678,656 to 679,789
Atu3625
Atu3626: Atu3626 - shikimate kinase, at 679,795 to 680,376
Atu3626
Atu3627: Atu3627 - hypothetical protein, at 680,373 to 680,531
Atu3627
Atu3628: Atu3628 - hypothetical protein, at 680,564 to 680,737
Atu3628
Atu3629: Atu3629 - site-specific tyrosine recombinase XerD, at 680,734 to 681,729
Atu3629
Position (kb) 680
681 Strain fitness (log2 ratio) -1
0
1 at 679.814 kb on - strand at 680.413 kb on + strand, within Atu3627 at 680.489 kb on - strand, within Atu3627 at 680.614 kb on + strand, within Atu3628 at 680.638 kb on + strand, within Atu3628 at 680.646 kb on - strand, within Atu3628 at 680.661 kb on + strand, within Atu3628 at 680.667 kb on + strand, within Atu3628 at 680.685 kb on + strand, within Atu3628 at 680.702 kb on - strand, within Atu3628 at 680.717 kb on - strand, within Atu3628 at 680.738 kb on - strand at 680.745 kb on + strand at 680.753 kb on - strand at 680.753 kb on - strand at 680.772 kb on + strand at 680.772 kb on + strand at 680.780 kb on - strand at 680.780 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hygromycin B 12.5 ug/mL remove 679,814 - -1.0 680,413 + Atu3627 0.25 -0.5 680,489 - Atu3627 0.73 -0.8 680,614 + Atu3628 0.29 +0.5 680,638 + Atu3628 0.43 +0.4 680,646 - Atu3628 0.47 +0.0 680,661 + Atu3628 0.56 +0.3 680,667 + Atu3628 0.59 -0.7 680,685 + Atu3628 0.70 -0.0 680,702 - Atu3628 0.79 +0.0 680,717 - Atu3628 0.88 -0.0 680,738 - -0.5 680,745 + +0.3 680,753 - +0.5 680,753 - -0.7 680,772 + -0.2 680,772 + +0.3 680,780 - +0.0 680,780 - +1.3
Or see this region's nucleotide sequence