Experiment: Hygromycin B 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3062 and Atu3063 are separated by 32 nucleotides Atu3063 and Atu3064 overlap by 4 nucleotides Atu3064 and Atu3065 overlap by 4 nucleotides
Atu3062: Atu3062 - LacI family transcriptional regulator, at 64,913 to 65,941
Atu3062
Atu3063: Atu3063 - ABC transporter permease, at 65,974 to 66,927
Atu3063
Atu3064: Atu3064 - ABC transporter permease, at 66,924 to 67,727
Atu3064
Atu3065: Atu3065 - sugar ABC transporter permease, at 67,724 to 68,728
Atu3065
Position (kb) 66
67
68 Strain fitness (log2 ratio) -2
-1
0
1
2 at 66.049 kb on + strand at 66.049 kb on + strand at 66.182 kb on - strand, within Atu3063 at 66.199 kb on + strand, within Atu3063 at 66.199 kb on + strand, within Atu3063 at 66.199 kb on + strand, within Atu3063 at 66.207 kb on - strand, within Atu3063 at 66.207 kb on - strand, within Atu3063 at 66.207 kb on - strand, within Atu3063 at 66.207 kb on - strand, within Atu3063 at 66.207 kb on - strand, within Atu3063 at 66.207 kb on - strand, within Atu3063 at 66.251 kb on - strand, within Atu3063 at 66.381 kb on + strand, within Atu3063 at 66.456 kb on - strand, within Atu3063 at 66.477 kb on - strand, within Atu3063 at 66.571 kb on + strand, within Atu3063 at 66.801 kb on - strand, within Atu3063 at 66.881 kb on - strand at 66.939 kb on - strand at 66.949 kb on - strand at 66.949 kb on - strand at 67.286 kb on + strand, within Atu3064 at 67.402 kb on + strand, within Atu3064 at 67.461 kb on + strand, within Atu3064 at 67.479 kb on + strand, within Atu3064 at 67.559 kb on + strand, within Atu3064 at 67.567 kb on - strand, within Atu3064 at 67.573 kb on + strand, within Atu3064 at 67.679 kb on - strand at 67.711 kb on - strand at 67.729 kb on + strand at 67.861 kb on - strand, within Atu3065 at 67.879 kb on - strand, within Atu3065 at 67.909 kb on - strand, within Atu3065 at 67.955 kb on - strand, within Atu3065 at 67.989 kb on - strand, within Atu3065 at 68.083 kb on - strand, within Atu3065 at 68.083 kb on - strand, within Atu3065 at 68.083 kb on - strand, within Atu3065 at 68.085 kb on + strand, within Atu3065 at 68.144 kb on - strand, within Atu3065 at 68.151 kb on + strand, within Atu3065 at 68.161 kb on + strand, within Atu3065 at 68.169 kb on - strand, within Atu3065 at 68.173 kb on + strand, within Atu3065 at 68.173 kb on + strand, within Atu3065 at 68.173 kb on + strand, within Atu3065 at 68.177 kb on - strand, within Atu3065 at 68.255 kb on - strand, within Atu3065 at 68.391 kb on + strand, within Atu3065 at 68.392 kb on + strand, within Atu3065 at 68.434 kb on + strand, within Atu3065 at 68.474 kb on + strand, within Atu3065 at 68.495 kb on - strand, within Atu3065 at 68.561 kb on + strand, within Atu3065 at 68.561 kb on + strand, within Atu3065 at 68.569 kb on - strand, within Atu3065 at 68.569 kb on - strand, within Atu3065 at 68.569 kb on - strand, within Atu3065 at 68.569 kb on - strand, within Atu3065 at 68.569 kb on - strand, within Atu3065 at 68.571 kb on - strand, within Atu3065 at 68.598 kb on + strand, within Atu3065 at 68.633 kb on - strand at 68.639 kb on - strand at 68.683 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hygromycin B 12.5 ug/mL remove 66,049 + -0.5 66,049 + -0.5 66,182 - Atu3063 0.22 -0.8 66,199 + Atu3063 0.24 -0.2 66,199 + Atu3063 0.24 -0.4 66,199 + Atu3063 0.24 -0.5 66,207 - Atu3063 0.24 +0.1 66,207 - Atu3063 0.24 -0.4 66,207 - Atu3063 0.24 +0.5 66,207 - Atu3063 0.24 -0.7 66,207 - Atu3063 0.24 -0.9 66,207 - Atu3063 0.24 -1.9 66,251 - Atu3063 0.29 +0.7 66,381 + Atu3063 0.43 -0.6 66,456 - Atu3063 0.51 +0.0 66,477 - Atu3063 0.53 -1.1 66,571 + Atu3063 0.63 -0.3 66,801 - Atu3063 0.87 -1.1 66,881 - +0.7 66,939 - -0.6 66,949 - +0.2 66,949 - -0.4 67,286 + Atu3064 0.45 -0.9 67,402 + Atu3064 0.59 -0.9 67,461 + Atu3064 0.67 -0.4 67,479 + Atu3064 0.69 -0.4 67,559 + Atu3064 0.79 -1.9 67,567 - Atu3064 0.80 -0.6 67,573 + Atu3064 0.81 -0.2 67,679 - +0.0 67,711 - -0.3 67,729 + +0.6 67,861 - Atu3065 0.14 -1.2 67,879 - Atu3065 0.15 -0.3 67,909 - Atu3065 0.18 +0.1 67,955 - Atu3065 0.23 +0.8 67,989 - Atu3065 0.26 +2.3 68,083 - Atu3065 0.36 -0.7 68,083 - Atu3065 0.36 -0.5 68,083 - Atu3065 0.36 -0.2 68,085 + Atu3065 0.36 +0.5 68,144 - Atu3065 0.42 -0.8 68,151 + Atu3065 0.42 -0.2 68,161 + Atu3065 0.43 -0.1 68,169 - Atu3065 0.44 -1.3 68,173 + Atu3065 0.45 -0.1 68,173 + Atu3065 0.45 -0.4 68,173 + Atu3065 0.45 -0.3 68,177 - Atu3065 0.45 +0.4 68,255 - Atu3065 0.53 -0.9 68,391 + Atu3065 0.66 -0.4 68,392 + Atu3065 0.66 -2.2 68,434 + Atu3065 0.71 -0.1 68,474 + Atu3065 0.75 +1.3 68,495 - Atu3065 0.77 -0.4 68,561 + Atu3065 0.83 +0.5 68,561 + Atu3065 0.83 +0.0 68,569 - Atu3065 0.84 -0.1 68,569 - Atu3065 0.84 +0.1 68,569 - Atu3065 0.84 +0.5 68,569 - Atu3065 0.84 +0.6 68,569 - Atu3065 0.84 -0.5 68,571 - Atu3065 0.84 +0.0 68,598 + Atu3065 0.87 +0.6 68,633 - -0.9 68,639 - +0.7 68,683 + +0.5
Or see this region's nucleotide sequence