Experiment: Hygromycin B 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2655 and Atu2656 are separated by 4 nucleotides Atu2656 and Atu2657 are separated by 14 nucleotides Atu2657 and Atu2658 overlap by 11 nucleotides
Atu2655: Atu2655 - hypothetical protein, at 2,637,429 to 2,638,874
Atu2655
Atu2656: Atu2656 - hypothetical protein, at 2,638,879 to 2,640,495
Atu2656
Atu2657: Atu2657 - hypothetical protein, at 2,640,510 to 2,640,998
Atu2657
Atu2658: Atu2658 - GMP synthase - glutamine amidotransferase protein, at 2,640,988 to 2,641,719
Atu2658
Position (kb)
2638
2639
2640
2641 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2637.927 kb on - strand, within Atu2655 at 2637.927 kb on - strand, within Atu2655 at 2637.927 kb on - strand, within Atu2655 at 2637.937 kb on - strand, within Atu2655 at 2637.938 kb on - strand, within Atu2655 at 2638.063 kb on + strand, within Atu2655 at 2638.445 kb on + strand, within Atu2655 at 2638.453 kb on - strand, within Atu2655 at 2638.453 kb on - strand, within Atu2655 at 2638.453 kb on - strand, within Atu2655 at 2638.453 kb on - strand, within Atu2655 at 2638.489 kb on - strand, within Atu2655 at 2638.490 kb on - strand, within Atu2655 at 2638.592 kb on + strand, within Atu2655 at 2638.639 kb on - strand, within Atu2655 at 2638.661 kb on + strand, within Atu2655 at 2638.706 kb on + strand, within Atu2655 at 2638.747 kb on + strand at 2638.838 kb on + strand at 2638.841 kb on - strand at 2638.957 kb on - strand at 2639.011 kb on + strand at 2639.112 kb on + strand, within Atu2656 at 2639.132 kb on + strand, within Atu2656 at 2639.132 kb on + strand, within Atu2656 at 2639.190 kb on + strand, within Atu2656 at 2639.265 kb on - strand, within Atu2656 at 2639.291 kb on - strand, within Atu2656 at 2639.347 kb on - strand, within Atu2656 at 2639.439 kb on + strand, within Atu2656 at 2639.447 kb on - strand, within Atu2656 at 2639.447 kb on - strand, within Atu2656 at 2639.447 kb on - strand, within Atu2656 at 2639.457 kb on - strand, within Atu2656 at 2639.495 kb on + strand, within Atu2656 at 2639.531 kb on + strand, within Atu2656 at 2639.567 kb on - strand, within Atu2656 at 2639.690 kb on + strand, within Atu2656 at 2639.733 kb on + strand, within Atu2656 at 2639.786 kb on - strand, within Atu2656 at 2639.831 kb on - strand, within Atu2656 at 2639.933 kb on - strand, within Atu2656 at 2639.967 kb on - strand, within Atu2656 at 2639.997 kb on + strand, within Atu2656 at 2640.018 kb on - strand, within Atu2656 at 2640.174 kb on - strand, within Atu2656 at 2640.174 kb on - strand, within Atu2656 at 2640.178 kb on + strand, within Atu2656 at 2640.232 kb on - strand, within Atu2656 at 2640.342 kb on + strand at 2640.350 kb on - strand at 2640.550 kb on + strand at 2640.581 kb on + strand, within Atu2657 at 2640.615 kb on - strand, within Atu2657 at 2640.719 kb on - strand, within Atu2657 at 2640.734 kb on - strand, within Atu2657 at 2641.056 kb on + strand at 2641.126 kb on - strand, within Atu2658 at 2641.128 kb on + strand, within Atu2658 at 2641.150 kb on + strand, within Atu2658 at 2641.328 kb on - strand, within Atu2658 at 2641.470 kb on + strand, within Atu2658 at 2641.487 kb on + strand, within Atu2658
Per-strain Table
Position Strand Gene LocusTag Fraction Hygromycin B 12.5 ug/mL remove 2,637,927 - Atu2655 0.34 +0.9 2,637,927 - Atu2655 0.34 -0.4 2,637,927 - Atu2655 0.34 -1.3 2,637,937 - Atu2655 0.35 -0.8 2,637,938 - Atu2655 0.35 -0.5 2,638,063 + Atu2655 0.44 +0.5 2,638,445 + Atu2655 0.70 +0.9 2,638,453 - Atu2655 0.71 -0.5 2,638,453 - Atu2655 0.71 -1.1 2,638,453 - Atu2655 0.71 -0.0 2,638,453 - Atu2655 0.71 +0.5 2,638,489 - Atu2655 0.73 -0.5 2,638,490 - Atu2655 0.73 -1.3 2,638,592 + Atu2655 0.80 -0.4 2,638,639 - Atu2655 0.84 -0.4 2,638,661 + Atu2655 0.85 -1.6 2,638,706 + Atu2655 0.88 -0.2 2,638,747 + -0.6 2,638,838 + +2.5 2,638,841 - +0.9 2,638,957 - -1.2 2,639,011 + -1.1 2,639,112 + Atu2656 0.14 +0.5 2,639,132 + Atu2656 0.16 -0.8 2,639,132 + Atu2656 0.16 -0.5 2,639,190 + Atu2656 0.19 +0.5 2,639,265 - Atu2656 0.24 -0.7 2,639,291 - Atu2656 0.25 +0.5 2,639,347 - Atu2656 0.29 -0.2 2,639,439 + Atu2656 0.35 -0.5 2,639,447 - Atu2656 0.35 +2.5 2,639,447 - Atu2656 0.35 +0.9 2,639,447 - Atu2656 0.35 -2.4 2,639,457 - Atu2656 0.36 -1.2 2,639,495 + Atu2656 0.38 -1.1 2,639,531 + Atu2656 0.40 -2.7 2,639,567 - Atu2656 0.43 +0.5 2,639,690 + Atu2656 0.50 -1.5 2,639,733 + Atu2656 0.53 -0.3 2,639,786 - Atu2656 0.56 -2.8 2,639,831 - Atu2656 0.59 -1.1 2,639,933 - Atu2656 0.65 +0.9 2,639,967 - Atu2656 0.67 +0.5 2,639,997 + Atu2656 0.69 -1.8 2,640,018 - Atu2656 0.70 -0.1 2,640,174 - Atu2656 0.80 +0.2 2,640,174 - Atu2656 0.80 +0.5 2,640,178 + Atu2656 0.80 -0.6 2,640,232 - Atu2656 0.84 -2.1 2,640,342 + -0.2 2,640,350 - -0.5 2,640,550 + -0.6 2,640,581 + Atu2657 0.15 +0.9 2,640,615 - Atu2657 0.21 +0.5 2,640,719 - Atu2657 0.43 +0.3 2,640,734 - Atu2657 0.46 -0.6 2,641,056 + -0.7 2,641,126 - Atu2658 0.19 -0.6 2,641,128 + Atu2658 0.19 +0.1 2,641,150 + Atu2658 0.22 +0.5 2,641,328 - Atu2658 0.46 -0.1 2,641,470 + Atu2658 0.66 +0.2 2,641,487 + Atu2658 0.68 +0.7
Or see this region's nucleotide sequence