Experiment: Hygromycin B 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1081 and Atu1082 are separated by 98 nucleotides Atu1082 and Atu1083 overlap by 4 nucleotides Atu1083 and Atu1084 are separated by 99 nucleotides
Atu1081: Atu1081 - transcriptional regulator, AraC family, at 1,072,651 to 1,073,472
Atu1081
Atu1082: Atu1082 - branched-chain amino acid permease, at 1,073,571 to 1,074,275
Atu1082
Atu1083: Atu1083 - hypothetical protein, at 1,074,272 to 1,074,574
Atu1083
Atu1084: Atu1084 - replicative DNA helicase, at 1,074,674 to 1,076,170
Atu1084
Position (kb)
1074
1075 Strain fitness (log2 ratio)
-1
0
1 at 1073.475 kb on - strand at 1073.521 kb on + strand at 1073.569 kb on - strand at 1073.607 kb on + strand at 1073.625 kb on + strand at 1073.633 kb on - strand at 1073.638 kb on + strand at 1073.760 kb on + strand, within Atu1082 at 1073.826 kb on + strand, within Atu1082 at 1073.834 kb on - strand, within Atu1082 at 1073.866 kb on - strand, within Atu1082 at 1073.959 kb on - strand, within Atu1082 at 1073.992 kb on - strand, within Atu1082 at 1074.015 kb on - strand, within Atu1082 at 1074.034 kb on + strand, within Atu1082 at 1074.109 kb on - strand, within Atu1082 at 1074.178 kb on + strand, within Atu1082 at 1074.178 kb on + strand, within Atu1082 at 1074.190 kb on - strand, within Atu1082 at 1074.237 kb on + strand at 1074.293 kb on + strand at 1074.310 kb on - strand, within Atu1083 at 1074.324 kb on + strand, within Atu1083 at 1074.370 kb on - strand, within Atu1083 at 1074.456 kb on - strand, within Atu1083 at 1074.467 kb on + strand, within Atu1083 at 1074.467 kb on + strand, within Atu1083 at 1074.641 kb on + strand at 1074.699 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hygromycin B 12.5 ug/mL remove 1,073,475 - +1.0 1,073,521 + +0.2 1,073,569 - +0.7 1,073,607 + +0.3 1,073,625 + -0.1 1,073,633 - -0.7 1,073,638 + -0.4 1,073,760 + Atu1082 0.27 -0.3 1,073,826 + Atu1082 0.36 +0.5 1,073,834 - Atu1082 0.37 +0.5 1,073,866 - Atu1082 0.42 +0.5 1,073,959 - Atu1082 0.55 -0.1 1,073,992 - Atu1082 0.60 -0.6 1,074,015 - Atu1082 0.63 +0.3 1,074,034 + Atu1082 0.66 -0.2 1,074,109 - Atu1082 0.76 -0.1 1,074,178 + Atu1082 0.86 -0.4 1,074,178 + Atu1082 0.86 -0.2 1,074,190 - Atu1082 0.88 -0.1 1,074,237 + +0.8 1,074,293 + -0.5 1,074,310 - Atu1083 0.13 +0.2 1,074,324 + Atu1083 0.17 -0.1 1,074,370 - Atu1083 0.32 +0.9 1,074,456 - Atu1083 0.61 +0.4 1,074,467 + Atu1083 0.64 +0.9 1,074,467 + Atu1083 0.64 -0.7 1,074,641 + -0.2 1,074,699 - +0.3
Or see this region's nucleotide sequence