Experiment: Hygromycin B 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0895 and Atu0896 are separated by 30 nucleotides Atu0896 and Atu0897 are separated by 244 nucleotides Atu0897 and Atu0898 are separated by 222 nucleotides Atu0898 and Atu0899 are separated by 104 nucleotides
Atu0895: Atu0895 - ABC transporter, substrate binding protein, at 885,865 to 886,875
Atu0895
Atu0896: Atu0896 - hypothetical protein, at 886,906 to 887,127
Atu0896
Atu0897: Atu0897 - protease II, at 887,372 to 889,471
Atu0897
Atu0898: Atu0898 - hypothetical protein, at 889,694 to 890,095
Atu0898
Atu0899: Atu0899 - dihydrodipicolinate synthase, at 890,200 to 891,108
Atu0899
Position (kb) 887
888
889
890 Strain fitness (log2 ratio) -2
-1
0
1
2 at 886.559 kb on + strand, within Atu0895 at 886.729 kb on + strand, within Atu0895 at 886.737 kb on - strand, within Atu0895 at 886.839 kb on - strand at 886.919 kb on + strand at 887.109 kb on + strand at 887.369 kb on + strand at 887.416 kb on - strand at 887.491 kb on + strand at 887.656 kb on - strand, within Atu0897 at 887.705 kb on + strand, within Atu0897 at 887.875 kb on - strand, within Atu0897 at 887.930 kb on - strand, within Atu0897 at 887.937 kb on + strand, within Atu0897 at 887.955 kb on + strand, within Atu0897 at 888.001 kb on - strand, within Atu0897 at 888.009 kb on + strand, within Atu0897 at 888.009 kb on + strand, within Atu0897 at 888.009 kb on + strand, within Atu0897 at 888.017 kb on - strand, within Atu0897 at 888.043 kb on - strand, within Atu0897 at 888.093 kb on - strand, within Atu0897 at 888.154 kb on - strand, within Atu0897 at 888.192 kb on - strand, within Atu0897 at 888.197 kb on - strand, within Atu0897 at 888.226 kb on - strand, within Atu0897 at 888.324 kb on + strand, within Atu0897 at 888.382 kb on - strand, within Atu0897 at 888.382 kb on - strand, within Atu0897 at 888.492 kb on + strand, within Atu0897 at 888.608 kb on + strand, within Atu0897 at 888.639 kb on + strand, within Atu0897 at 888.653 kb on - strand, within Atu0897 at 888.657 kb on + strand, within Atu0897 at 888.665 kb on - strand, within Atu0897 at 888.703 kb on - strand, within Atu0897 at 888.808 kb on - strand, within Atu0897 at 888.823 kb on + strand, within Atu0897 at 888.832 kb on - strand, within Atu0897 at 888.865 kb on - strand, within Atu0897 at 888.935 kb on + strand, within Atu0897 at 888.935 kb on + strand, within Atu0897 at 888.943 kb on - strand, within Atu0897 at 888.969 kb on + strand, within Atu0897 at 889.056 kb on + strand, within Atu0897 at 889.084 kb on - strand, within Atu0897 at 889.119 kb on - strand, within Atu0897 at 889.192 kb on + strand, within Atu0897 at 889.197 kb on + strand, within Atu0897 at 889.229 kb on - strand, within Atu0897 at 889.254 kb on + strand, within Atu0897 at 889.298 kb on + strand at 889.298 kb on + strand at 889.330 kb on + strand at 889.332 kb on + strand at 889.338 kb on - strand at 889.340 kb on - strand at 889.365 kb on + strand at 889.400 kb on + strand at 889.514 kb on + strand at 889.515 kb on + strand at 889.574 kb on + strand at 889.607 kb on + strand at 889.631 kb on + strand at 889.803 kb on + strand, within Atu0898 at 889.811 kb on - strand, within Atu0898 at 889.815 kb on + strand, within Atu0898 at 889.815 kb on + strand, within Atu0898 at 889.823 kb on - strand, within Atu0898 at 889.823 kb on - strand, within Atu0898 at 889.833 kb on - strand, within Atu0898 at 889.852 kb on + strand, within Atu0898 at 889.888 kb on + strand, within Atu0898 at 889.896 kb on - strand, within Atu0898 at 889.911 kb on + strand, within Atu0898 at 889.952 kb on + strand, within Atu0898 at 889.960 kb on - strand, within Atu0898 at 889.960 kb on - strand, within Atu0898 at 889.962 kb on - strand, within Atu0898 at 890.016 kb on + strand, within Atu0898 at 890.021 kb on + strand, within Atu0898 at 890.029 kb on - strand, within Atu0898 at 890.169 kb on - strand at 890.183 kb on + strand at 890.191 kb on - strand at 890.298 kb on + strand, within Atu0899
Per-strain Table
Position Strand Gene LocusTag Fraction Hygromycin B 12.5 ug/mL remove 886,559 + Atu0895 0.69 -0.7 886,729 + Atu0895 0.85 +0.3 886,737 - Atu0895 0.86 +1.3 886,839 - +1.3 886,919 + +1.3 887,109 + +0.7 887,369 + -0.1 887,416 - +0.0 887,491 + -1.2 887,656 - Atu0897 0.14 +0.3 887,705 + Atu0897 0.16 -0.2 887,875 - Atu0897 0.24 -1.0 887,930 - Atu0897 0.27 -0.2 887,937 + Atu0897 0.27 -0.4 887,955 + Atu0897 0.28 -0.2 888,001 - Atu0897 0.30 -0.6 888,009 + Atu0897 0.30 -0.3 888,009 + Atu0897 0.30 -0.3 888,009 + Atu0897 0.30 -0.2 888,017 - Atu0897 0.31 +0.3 888,043 - Atu0897 0.32 -1.3 888,093 - Atu0897 0.34 -1.5 888,154 - Atu0897 0.37 -0.6 888,192 - Atu0897 0.39 -0.8 888,197 - Atu0897 0.39 -2.0 888,226 - Atu0897 0.41 +0.3 888,324 + Atu0897 0.45 +0.3 888,382 - Atu0897 0.48 -1.0 888,382 - Atu0897 0.48 +0.3 888,492 + Atu0897 0.53 -0.7 888,608 + Atu0897 0.59 +0.4 888,639 + Atu0897 0.60 -0.9 888,653 - Atu0897 0.61 +1.3 888,657 + Atu0897 0.61 +0.2 888,665 - Atu0897 0.62 -0.1 888,703 - Atu0897 0.63 -2.0 888,808 - Atu0897 0.68 +1.9 888,823 + Atu0897 0.69 -0.8 888,832 - Atu0897 0.70 +0.1 888,865 - Atu0897 0.71 -0.2 888,935 + Atu0897 0.74 -0.5 888,935 + Atu0897 0.74 -0.8 888,943 - Atu0897 0.75 -0.5 888,969 + Atu0897 0.76 -1.5 889,056 + Atu0897 0.80 -1.3 889,084 - Atu0897 0.82 -0.6 889,119 - Atu0897 0.83 -0.3 889,192 + Atu0897 0.87 -1.5 889,197 + Atu0897 0.87 -0.8 889,229 - Atu0897 0.88 -0.2 889,254 + Atu0897 0.90 +1.3 889,298 + -0.7 889,298 + -0.3 889,330 + +1.5 889,332 + -0.6 889,338 - -0.1 889,340 - +1.0 889,365 + -0.2 889,400 + +0.5 889,514 + -0.7 889,515 + -0.6 889,574 + +0.5 889,607 + +0.4 889,631 + -0.2 889,803 + Atu0898 0.27 -0.2 889,811 - Atu0898 0.29 +0.0 889,815 + Atu0898 0.30 +0.3 889,815 + Atu0898 0.30 -0.7 889,823 - Atu0898 0.32 +0.3 889,823 - Atu0898 0.32 -0.2 889,833 - Atu0898 0.35 +1.1 889,852 + Atu0898 0.39 +0.5 889,888 + Atu0898 0.48 +0.1 889,896 - Atu0898 0.50 +0.2 889,911 + Atu0898 0.54 -0.3 889,952 + Atu0898 0.64 -0.7 889,960 - Atu0898 0.66 +0.2 889,960 - Atu0898 0.66 +0.2 889,962 - Atu0898 0.67 -0.5 890,016 + Atu0898 0.80 +0.2 890,021 + Atu0898 0.81 +0.5 890,029 - Atu0898 0.83 +0.3 890,169 - +0.3 890,183 + +0.3 890,191 - -0.6 890,298 + Atu0899 0.11 -0.3
Or see this region's nucleotide sequence