Experiment: Erythromycin 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1125 and Atu1126 are separated by 52 nucleotides Atu1126 and Atu1127 are separated by 4 nucleotides Atu1127 and Atu1128 overlap by 1 nucleotides Atu1128 and Atu1129 are separated by 102 nucleotides
Atu1125: Atu1125 - ABC transporter, membrane spanning protein, at 1,115,058 to 1,115,954
Atu1125
Atu1126: Atu1126 - molybdopterin converting factor, large subunit, at 1,116,007 to 1,116,474
Atu1126
Atu1127: Atu1127 - molybdopterin converting factor small subunit, at 1,116,479 to 1,116,733
Atu1127
Atu1128: Atu1128 - phosphatidylglycerophosphate synthase, at 1,116,733 to 1,117,320
Atu1128
Atu1129: Atu1129 - excinuclease ABC chain C, at 1,117,423 to 1,119,351
Atu1129
Position (kb)
1116
1117
1118 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1115.810 kb on + strand, within Atu1125 at 1116.027 kb on - strand at 1116.076 kb on + strand, within Atu1126 at 1116.110 kb on + strand, within Atu1126 at 1116.175 kb on + strand, within Atu1126 at 1116.230 kb on - strand, within Atu1126 at 1116.243 kb on + strand, within Atu1126 at 1116.307 kb on + strand, within Atu1126 at 1116.333 kb on - strand, within Atu1126 at 1116.587 kb on + strand, within Atu1127 at 1116.595 kb on - strand, within Atu1127 at 1117.651 kb on + strand, within Atu1129 at 1117.777 kb on - strand, within Atu1129 at 1117.829 kb on - strand, within Atu1129 at 1117.843 kb on - strand, within Atu1129 at 1117.843 kb on - strand, within Atu1129 at 1117.880 kb on - strand, within Atu1129 at 1117.957 kb on - strand, within Atu1129 at 1118.160 kb on + strand, within Atu1129 at 1118.168 kb on - strand, within Atu1129
Per-strain Table
Position Strand Gene LocusTag Fraction Erythromycin 12.5 ug/mL remove 1,115,810 + Atu1125 0.84 +0.7 1,116,027 - -0.2 1,116,076 + Atu1126 0.15 -0.1 1,116,110 + Atu1126 0.22 +0.8 1,116,175 + Atu1126 0.36 -1.8 1,116,230 - Atu1126 0.48 +0.2 1,116,243 + Atu1126 0.50 -0.7 1,116,307 + Atu1126 0.64 +0.3 1,116,333 - Atu1126 0.70 +1.6 1,116,587 + Atu1127 0.42 +0.3 1,116,595 - Atu1127 0.45 +1.6 1,117,651 + Atu1129 0.12 -0.7 1,117,777 - Atu1129 0.18 +1.1 1,117,829 - Atu1129 0.21 +0.3 1,117,843 - Atu1129 0.22 +2.2 1,117,843 - Atu1129 0.22 -1.4 1,117,880 - Atu1129 0.24 -0.6 1,117,957 - Atu1129 0.28 +0.6 1,118,160 + Atu1129 0.38 +1.6 1,118,168 - Atu1129 0.39 -1.0
Or see this region's nucleotide sequence