Experiment: Erythromycin 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0297 and Atu0298 are separated by 20 nucleotides Atu0298 and Atu0299 are separated by 4 nucleotides Atu0299 and Atu0300 are separated by 96 nucleotides Atu0300 and Atu0301 are separated by 193 nucleotides
Atu0297: Atu0297 - hypothetical protein, at 291,257 to 291,544
Atu0297
Atu0298: Atu0298 - orotidine 5, at 291,565 to 292,272
Atu0298
Atu0299: Atu0299 - hypothetical protein, at 292,277 to 292,873
Atu0299
Atu0300: Atu0300 - methyltransferase, at 292,970 to 293,563
Atu0300
Atu0301: Atu0301 - DNA polymerase III, beta chain, at 293,757 to 294,875
Atu0301
Position (kb) 292
293 Strain fitness (log2 ratio) -2
-1
0
1 at 291.323 kb on + strand, within Atu0297 at 291.333 kb on - strand, within Atu0297 at 291.348 kb on + strand, within Atu0297 at 291.363 kb on + strand, within Atu0297 at 291.454 kb on + strand, within Atu0297 at 291.471 kb on + strand, within Atu0297 at 291.859 kb on + strand, within Atu0298 at 291.862 kb on - strand, within Atu0298 at 291.904 kb on - strand, within Atu0298 at 291.925 kb on + strand, within Atu0298 at 291.933 kb on - strand, within Atu0298 at 291.933 kb on - strand, within Atu0298 at 291.993 kb on - strand, within Atu0298 at 292.000 kb on - strand, within Atu0298 at 292.022 kb on + strand, within Atu0298 at 292.064 kb on + strand, within Atu0298 at 292.072 kb on - strand, within Atu0298 at 292.072 kb on - strand, within Atu0298 at 292.074 kb on - strand, within Atu0298 at 292.083 kb on + strand, within Atu0298 at 292.182 kb on + strand, within Atu0298 at 292.210 kb on + strand at 292.227 kb on - strand at 292.268 kb on + strand at 292.274 kb on + strand at 292.275 kb on - strand at 292.409 kb on + strand, within Atu0299 at 292.421 kb on + strand, within Atu0299 at 292.422 kb on + strand, within Atu0299 at 292.461 kb on - strand, within Atu0299 at 292.471 kb on + strand, within Atu0299 at 292.479 kb on - strand, within Atu0299 at 292.489 kb on - strand, within Atu0299 at 292.555 kb on - strand, within Atu0299 at 292.691 kb on + strand, within Atu0299 at 292.746 kb on + strand, within Atu0299 at 292.755 kb on - strand, within Atu0299 at 292.757 kb on + strand, within Atu0299 at 292.825 kb on - strand at 292.836 kb on + strand at 292.842 kb on + strand at 292.852 kb on + strand at 292.862 kb on - strand at 292.910 kb on - strand at 292.910 kb on - strand at 292.986 kb on + strand at 293.054 kb on - strand, within Atu0300 at 293.108 kb on - strand, within Atu0300 at 293.186 kb on + strand, within Atu0300 at 293.193 kb on - strand, within Atu0300 at 293.201 kb on + strand, within Atu0300 at 293.208 kb on + strand, within Atu0300 at 293.216 kb on - strand, within Atu0300 at 293.285 kb on - strand, within Atu0300 at 293.302 kb on - strand, within Atu0300 at 293.330 kb on + strand, within Atu0300 at 293.366 kb on + strand, within Atu0300 at 293.492 kb on + strand, within Atu0300 at 293.545 kb on + strand at 293.553 kb on - strand at 293.553 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Erythromycin 12.5 ug/mL remove 291,323 + Atu0297 0.23 -0.2 291,333 - Atu0297 0.26 -0.6 291,348 + Atu0297 0.32 +0.3 291,363 + Atu0297 0.37 -0.2 291,454 + Atu0297 0.68 +1.1 291,471 + Atu0297 0.74 +0.1 291,859 + Atu0298 0.42 -0.6 291,862 - Atu0298 0.42 -1.1 291,904 - Atu0298 0.48 +0.0 291,925 + Atu0298 0.51 -1.6 291,933 - Atu0298 0.52 -0.9 291,933 - Atu0298 0.52 -0.8 291,993 - Atu0298 0.60 -0.0 292,000 - Atu0298 0.61 +0.1 292,022 + Atu0298 0.65 -1.3 292,064 + Atu0298 0.70 -1.3 292,072 - Atu0298 0.72 -1.4 292,072 - Atu0298 0.72 -0.3 292,074 - Atu0298 0.72 -0.2 292,083 + Atu0298 0.73 -0.2 292,182 + Atu0298 0.87 +1.7 292,210 + -1.9 292,227 - -0.6 292,268 + +0.1 292,274 + +0.4 292,275 - +0.3 292,409 + Atu0299 0.22 -0.3 292,421 + Atu0299 0.24 -2.6 292,422 + Atu0299 0.24 -0.5 292,461 - Atu0299 0.31 +0.0 292,471 + Atu0299 0.32 -1.1 292,479 - Atu0299 0.34 -0.4 292,489 - Atu0299 0.36 -0.1 292,555 - Atu0299 0.47 -0.9 292,691 + Atu0299 0.69 -0.2 292,746 + Atu0299 0.79 +0.0 292,755 - Atu0299 0.80 -0.6 292,757 + Atu0299 0.80 -0.8 292,825 - +0.1 292,836 + -0.3 292,842 + +0.5 292,852 + -0.1 292,862 - +1.7 292,910 - -2.0 292,910 - -0.3 292,986 + -0.2 293,054 - Atu0300 0.14 -0.6 293,108 - Atu0300 0.23 +1.2 293,186 + Atu0300 0.36 -0.2 293,193 - Atu0300 0.38 +0.1 293,201 + Atu0300 0.39 +0.6 293,208 + Atu0300 0.40 +0.8 293,216 - Atu0300 0.41 -0.5 293,285 - Atu0300 0.53 -2.5 293,302 - Atu0300 0.56 -1.1 293,330 + Atu0300 0.61 -0.6 293,366 + Atu0300 0.67 +0.5 293,492 + Atu0300 0.88 -0.8 293,545 + +0.2 293,553 - -0.1 293,553 - -0.2
Or see this region's nucleotide sequence