Experiment: Erythromycin 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0195 and Atu0196 are separated by 105 nucleotides Atu0196 and Atu0197 overlap by 4 nucleotides Atu0197 and Atu0198 overlap by 4 nucleotides
Atu0195: Atu0195 - gluconokinase, at 199,684 to 200,175
Atu0195
Atu0196: Atu0196 - ABC transporter, membrane spanning protein (proline/glycine/betaine), at 200,281 to 201,021
Atu0196
Atu0197: Atu0197 - ABC transporter, nucleotide binding/ATPase protein (proline/glycine/betaine), at 201,018 to 201,956
Atu0197
Atu0198: Atu0198 - ABC transporter, membrane spanning protein (proline/glycine/betaine), at 201,953 to 203,098
Atu0198
Position (kb)
201
202 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 200.134 kb on + strand at 200.141 kb on + strand at 200.149 kb on - strand at 200.199 kb on + strand at 200.207 kb on - strand at 200.395 kb on + strand, within Atu0196 at 200.481 kb on + strand, within Atu0196 at 200.544 kb on + strand, within Atu0196 at 200.605 kb on - strand, within Atu0196 at 200.605 kb on - strand, within Atu0196 at 200.605 kb on - strand, within Atu0196 at 200.813 kb on + strand, within Atu0196 at 200.816 kb on - strand, within Atu0196 at 200.816 kb on - strand, within Atu0196 at 200.865 kb on + strand, within Atu0196 at 200.889 kb on + strand, within Atu0196 at 201.066 kb on + strand at 201.085 kb on - strand at 201.128 kb on - strand, within Atu0197 at 201.134 kb on + strand, within Atu0197 at 201.181 kb on - strand, within Atu0197 at 201.207 kb on + strand, within Atu0197 at 201.215 kb on - strand, within Atu0197 at 201.306 kb on + strand, within Atu0197 at 201.306 kb on + strand, within Atu0197 at 201.429 kb on + strand, within Atu0197 at 201.429 kb on + strand, within Atu0197 at 201.437 kb on - strand, within Atu0197 at 201.524 kb on - strand, within Atu0197 at 201.586 kb on - strand, within Atu0197 at 201.597 kb on - strand, within Atu0197 at 201.691 kb on + strand, within Atu0197 at 201.853 kb on - strand, within Atu0197 at 201.874 kb on - strand at 201.970 kb on + strand at 202.000 kb on - strand at 202.001 kb on + strand at 202.050 kb on + strand at 202.060 kb on + strand at 202.060 kb on + strand at 202.067 kb on + strand at 202.067 kb on + strand at 202.067 kb on + strand at 202.075 kb on - strand, within Atu0198 at 202.163 kb on - strand, within Atu0198 at 202.243 kb on + strand, within Atu0198 at 202.251 kb on - strand, within Atu0198 at 202.251 kb on - strand, within Atu0198 at 202.251 kb on - strand, within Atu0198 at 202.382 kb on - strand, within Atu0198 at 202.434 kb on + strand, within Atu0198 at 202.442 kb on - strand, within Atu0198 at 202.442 kb on - strand, within Atu0198 at 202.468 kb on - strand, within Atu0198 at 202.481 kb on + strand, within Atu0198 at 202.481 kb on + strand, within Atu0198 at 202.481 kb on + strand, within Atu0198 at 202.481 kb on + strand, within Atu0198 at 202.481 kb on + strand, within Atu0198 at 202.489 kb on - strand, within Atu0198 at 202.510 kb on + strand, within Atu0198 at 202.596 kb on - strand, within Atu0198 at 202.623 kb on + strand, within Atu0198 at 202.631 kb on - strand, within Atu0198 at 202.722 kb on + strand, within Atu0198 at 202.730 kb on - strand, within Atu0198 at 202.766 kb on - strand, within Atu0198 at 202.824 kb on - strand, within Atu0198 at 202.956 kb on + strand, within Atu0198
Per-strain Table
Position Strand Gene LocusTag Fraction Erythromycin 12.5 ug/mL remove 200,134 + +2.0 200,141 + +0.3 200,149 - -0.3 200,199 + -0.2 200,207 - -0.0 200,395 + Atu0196 0.15 +0.5 200,481 + Atu0196 0.27 +0.1 200,544 + Atu0196 0.35 +0.2 200,605 - Atu0196 0.44 -0.0 200,605 - Atu0196 0.44 -0.2 200,605 - Atu0196 0.44 +0.0 200,813 + Atu0196 0.72 -0.5 200,816 - Atu0196 0.72 -1.0 200,816 - Atu0196 0.72 +0.2 200,865 + Atu0196 0.79 +0.3 200,889 + Atu0196 0.82 -0.9 201,066 + -0.9 201,085 - -0.6 201,128 - Atu0197 0.12 -0.8 201,134 + Atu0197 0.12 -0.1 201,181 - Atu0197 0.17 -0.8 201,207 + Atu0197 0.20 -0.6 201,215 - Atu0197 0.21 -1.8 201,306 + Atu0197 0.31 +0.5 201,306 + Atu0197 0.31 -2.8 201,429 + Atu0197 0.44 -0.0 201,429 + Atu0197 0.44 +1.0 201,437 - Atu0197 0.45 -0.6 201,524 - Atu0197 0.54 +0.9 201,586 - Atu0197 0.60 -1.1 201,597 - Atu0197 0.62 -0.9 201,691 + Atu0197 0.72 -0.5 201,853 - Atu0197 0.89 -0.8 201,874 - +0.2 201,970 + +0.2 202,000 - -0.9 202,001 + -0.3 202,050 + -0.1 202,060 + -0.1 202,060 + -0.5 202,067 + +0.3 202,067 + -1.1 202,067 + -0.2 202,075 - Atu0198 0.11 +0.4 202,163 - Atu0198 0.18 -0.2 202,243 + Atu0198 0.25 -0.1 202,251 - Atu0198 0.26 -0.2 202,251 - Atu0198 0.26 -0.6 202,251 - Atu0198 0.26 +1.7 202,382 - Atu0198 0.37 +0.1 202,434 + Atu0198 0.42 +0.8 202,442 - Atu0198 0.43 +0.3 202,442 - Atu0198 0.43 -0.6 202,468 - Atu0198 0.45 +0.1 202,481 + Atu0198 0.46 +0.2 202,481 + Atu0198 0.46 -0.7 202,481 + Atu0198 0.46 -1.5 202,481 + Atu0198 0.46 -1.3 202,481 + Atu0198 0.46 -0.5 202,489 - Atu0198 0.47 -0.2 202,510 + Atu0198 0.49 +0.6 202,596 - Atu0198 0.56 -0.4 202,623 + Atu0198 0.58 +0.7 202,631 - Atu0198 0.59 -0.1 202,722 + Atu0198 0.67 +1.2 202,730 - Atu0198 0.68 -0.3 202,766 - Atu0198 0.71 +0.1 202,824 - Atu0198 0.76 -0.1 202,956 + Atu0198 0.88 +0.0
Or see this region's nucleotide sequence