Experiment: Erythromycin 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu8155 and Atu1316 overlap by 49 nucleotides Atu1316 and Atu1317 are separated by 5 nucleotides Atu1317 and Atu1318 are separated by 126 nucleotides Atu1318 and Atu1319 are separated by 56 nucleotides
Atu8155: Atu8155 - hypothetical protein, at 1,306,585 to 1,306,902
Atu8155
Atu1316: Atu1316 - hypothetical protein, at 1,306,854 to 1,307,333
Atu1316
Atu1317: Atu1317 - metal dependent hydrolase, at 1,307,339 to 1,308,439
Atu1317
Atu1318: Atu1318 - glutamyl-tRNA-Gln-amidotransferase chain C, at 1,308,566 to 1,308,853
Atu1318
Atu1319: Atu1319 - glutamyl-tRNA amidotransferase subunit A, at 1,308,910 to 1,310,391
Atu1319
Position (kb)
1307
1308
1309 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1306.416 kb on - strand at 1306.442 kb on + strand at 1306.442 kb on + strand at 1306.450 kb on - strand at 1306.450 kb on - strand at 1306.450 kb on - strand at 1306.513 kb on + strand at 1306.602 kb on + strand at 1306.624 kb on + strand, within Atu8155 at 1306.637 kb on + strand, within Atu8155 at 1306.807 kb on + strand, within Atu8155 at 1307.377 kb on + strand at 1307.377 kb on + strand at 1307.377 kb on + strand at 1307.378 kb on + strand at 1307.393 kb on - strand at 1307.395 kb on + strand at 1307.395 kb on + strand at 1307.489 kb on + strand, within Atu1317 at 1307.707 kb on + strand, within Atu1317 at 1307.729 kb on - strand, within Atu1317 at 1307.863 kb on - strand, within Atu1317 at 1307.876 kb on + strand, within Atu1317 at 1308.007 kb on + strand, within Atu1317 at 1308.039 kb on + strand, within Atu1317 at 1308.080 kb on - strand, within Atu1317 at 1308.200 kb on - strand, within Atu1317
Per-strain Table
Position Strand Gene LocusTag Fraction Erythromycin 12.5 ug/mL remove 1,306,416 - -0.5 1,306,442 + +0.5 1,306,442 + +0.4 1,306,450 - +1.0 1,306,450 - +0.5 1,306,450 - -0.0 1,306,513 + +1.9 1,306,602 + +1.0 1,306,624 + Atu8155 0.12 +0.8 1,306,637 + Atu8155 0.16 +0.7 1,306,807 + Atu8155 0.70 +1.3 1,307,377 + -0.0 1,307,377 + -0.7 1,307,377 + -0.4 1,307,378 + +1.3 1,307,393 - +0.8 1,307,395 + +0.3 1,307,395 + +0.2 1,307,489 + Atu1317 0.14 -2.3 1,307,707 + Atu1317 0.33 -0.1 1,307,729 - Atu1317 0.35 -0.2 1,307,863 - Atu1317 0.48 +0.3 1,307,876 + Atu1317 0.49 -1.3 1,308,007 + Atu1317 0.61 -1.2 1,308,039 + Atu1317 0.64 -1.3 1,308,080 - Atu1317 0.67 +0.1 1,308,200 - Atu1317 0.78 -0.1
Or see this region's nucleotide sequence